Transferable Resistance to Clindamycin, Erythromycin, and Tetracycline in
            <i>Clostridium difficile</i>

Wüst, J; Hardegger, U

doi:10.1128/aac.23.5.784

Cited by 91 publications

(36 citation statements)

References 5 publications

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“…C. perfringens isolates were from France (6), Wisconsin (27), Germany (33), and Belgium (G. Daubé, Université de Liège). C. difficile isolates were from Australia (4), Europe (12,36), and Japan (23). Streptococcal isolates were from France (P. Courvalin, Institut Pasteur).…”

Section: Methodsmentioning

confidence: 99%

The closely related ermB-ermAM genes from Clostridium perfringens, Enterococcus faecalis (pAM beta 1), and Streptococcus agalactiae (pIP501) are flanked by variants of a directly repeated sequence

Berryman

Rood

1995

Antimicrob Agents Chemother

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The Clostridium perfringens macrolide-lincosamide-streptogramin B resistance gene, ermBP, was sequenced and shown to be identical to the ermB-ermAM gene from the promiscuous Enterococcus faecalis plasmid pAM␤1 and to have at least 98% nucleotide sequence identity to other ermB-ermAM genes. Flanking the ermBP structural gene were almost identical directly repeated 1,341-bp sequences (DR1 and DR2). These repeats potentially encoded a 298 (or 284)-amino-acid protein that had sequence similarity to chromosomal and plasmid partitioning proteins. The pAM␤1 and Streptococcus agalactiae (pIP501) erm determinants appeared to have DR2 but had similar internal 973-or 956-bp deletions in DR1, respectively. Some of the other ermB-ermAM class determinants had small portions of DR1, but none had complete copies. It is postulated that the C. perfringens ermBP determinant was derived from an enterococcal or streptococcal determinant that had complete copies of both DR1 and DR2.

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Section: Methodsmentioning

confidence: 99%

The closely related ermB-ermAM genes from Clostridium perfringens, Enterococcus faecalis (pAM beta 1), and Streptococcus agalactiae (pIP501) are flanked by variants of a directly repeated sequence

Berryman

Rood

1995

Antimicrob Agents Chemother

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“…In strain 630, 2 copies of this gene are located on the mobilizable non-conjugative Tn5398, previously shown to be transferable by a conjugation-like mechanism. [1][2][3] Even though this element has been extensively studied, its transfer mechanism is still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Integration oferm(B)-containing elements through large chromosome fragment exchange inClostridium difficile

Wasels

Spigaglia

Barbanti

et al. 2015

Mobile Genetic Elements

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In Clostridium difficile, erm(B) genes are located on mobile elements like Tn5398 and Tn6215. In previous studies, some of these elements were transferred by conjugation-like mechanisms, mobilized in trans by helper conjugative systems. In this study, we analyzed the genomes of several recipient strains that acquired either Tn5398 or Tn6215-like elements. We demonstrated that the integration of the transposons in the genome of the recipient cell was always due to homologous recombination events, involving exchange of large chromosomal segments. We did not observed transposon transfer to a C. difficile strain in presence of DNAse, suggesting that a possible transformation-like mechanism occurred in this recipient.

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“…More recently, erythromycin-resistant isolates have been isolated from a number of Clostridium spp. (12,31,33,36,41). Although the response of many of these strains to the streptogramin B antibiotics has not been reported, many isolates are also resistant to the lincosamide antibiotics, clindamycin and lincomycin.…”

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confidence: 99%

Cloning and hybridization analysis of ermP, a macrolide-lincosamide-streptogramin B resistance determinant from Clostridium perfringens

Berryman

Rood

1989

Antimicrob Agents Chemother

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The erythromycin resistance determinant from Clostridium perfringens CP592 was cloned and shown to be expressed in Escherichia coli. The resultant plasmid, pJIR122 (7.9 kilobase pairs [kb]), was unstable since in both recA+ and recA E. coli hosts spontaneous deletion of 2.7 kb, including the erythromycin resistance determinant, was observed. Subcloning, as well as deletion analysis with BAL 31, localized the erythromycin resistance gene (ermP) to within a 1.0-kb region of pjIR122. A 0.5-kb fragment internal to ermP was 32p labeled and used as an ermP-specific probe in DNA hybridization experiments which used target DNA prepared from representatives of each of the known erm classes and also from erythromycin-resistant isolates of a variety of clostridial species. Hybridizing sequences were detected in DNA from several Clostridium difficile isolates and a Clostridium paraputrificum strain; however, ermP was not widespread in erythromycin-resistant C.perfringens isolates. The ermP determinant hybridized to, and shared significant restriction identity with, the ermB class gene from the streptococcal plasmid pAM,ll. No hybridization was detected with the other six hybridization classes of erm determinants.

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Transferable Resistance to Clindamycin, Erythromycin, and Tetracycline in Clostridium difficile

Cited by 91 publications

References 5 publications

The closely related ermB-ermAM genes from Clostridium perfringens, Enterococcus faecalis (pAM beta 1), and Streptococcus agalactiae (pIP501) are flanked by variants of a directly repeated sequence

The closely related ermB-ermAM genes from Clostridium perfringens, Enterococcus faecalis (pAM beta 1), and Streptococcus agalactiae (pIP501) are flanked by variants of a directly repeated sequence

Integration oferm(B)-containing elements through large chromosome fragment exchange inClostridium difficile

Cloning and hybridization analysis of ermP, a macrolide-lincosamide-streptogramin B resistance determinant from Clostridium perfringens

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