Transferrin changes in haemodialysed patients

Abstract

Transferrin (Tf) is a glycoprotein responsible for iron transport in the human body. Physiologically in reaction with Concanavalin A, Tf occurs in four distinct variants Tf1, Tf2, Tf3 (apo-Tf) and Tf4. It was reported recently that Tf is changing, particularly during acute phase response, taking place among others in end-stage renal disease. In this study, we wanted to find the answer to three main questions: firstly, how Tf is changing in patients treated with maintenance haemodialysis (mHD), secondly, whether there are any Tf changes in the course of mHD treatment, and thirdly, what factors can affect Tf microheterogeneity in these patients. Studies were performed on 80 haemodialysed patients and 21 healthy volunteers. The Tf concentration was determined by the rocket immunoelectrophoresis, and its microheterogeneity was assessed by the ConA crossed immunoaffinity electrophoresis. During the annual observation of the distribution of the Tf variants, we have found both changes of the percentage contents of all Tf variants in the whole Tf concentration and a significant decrease in Tf2, Tf3 and Tf4 serum concentrations. Moreover, we found that decrease in the renal function, duration of mHD, and inflammation may contribute to these above-mentioned changes, which are probably the factors that should be taken into account when explaining the mechanisms of persistence of anaemia in haemodialysed patients.

Biospecific Self‐Assembly of a Nanoparticle Coating for Targeted and Stimuli‐Responsive Drug Delivery

“…Con A is known to bind a variety of sugars and glycoproteins and thus provides a means to self‐assemble various functionalities to the Con A–glycogen coating. In this study, we examined the incorporation of transferrin glycoproteins into the coating as a means to confer targeting and cellular uptake functionality . Initial evidence that Con A recognizes and binds transferrin is provided by the photographs in Figure A.…”

T-Cell Activation and Malnutrition Adversely Impact on Endothelial Progenitor Cell Mobilization in Patients on Extracorporeal Maintenance Dialysis Therapy

“…The malnutrition-inflammation score (MIS) of the International Society of Renal Nutrition and Metabolism, which is one of the CKD-specific nutritional scoring systems includes this parameter in its biochemical panel. Serum concentration of this molecule is diminished from 30 to 50% in CKD patients by comparison with healthy controls [46] . Inflammatory process and protein-energy wasting are potential responsible factors of such impairment.…”

“…As chronic inflammation impacts on protein-energy wasting in CKD [47] , it may be therefore a main factor influencing transferrin concentration [26,27] . Evidences of this impact are the negative correlations between transferrin and CRP or IL-6 concentrations in HD patients [46,48] . The negative correlation between CD38 bright expression on CD8 memory T lymphocytes and transferrin concentration corroborated this negative influence of chronic inflammation on nutritional status.…”

The Content of Reticulocyte Hemoglobin and Serum Concentration of the Soluble Transferrin Receptor for Diagnostics of Anemia in Chronically Hemodialyzed Patients

“…The major reasons for anemia in patients with chronic kidney disease are the reduction EPO production, chronic inflammation, shortened half-life of erythrocytes and iron deficiency [12,13]. Intravenous iron is a valid element in the treatment of ESRD.…”