Transformable Thymine-Requiring Mutant of
            <i>Bacillus subtilis</i>

Farmer, James L.; Rothman, Frank G.

doi:10.1128/jb.89.1.262-263.1965

Cited by 147 publications

(25 citation statements)

References 0 publications

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“…Genetics (thyA). Aminopterin selection seems to be generally applicable and has been used to isolate thymine-requiring mutants in a variety of microorganisms: Salmonella typhimurium (124), Aerobacter aerogenes (176) (200); B. megaterium (382), and B. subtilis (128).…”

Section: Biosynthesis Of Dttpmentioning

confidence: 99%

Pyrimidine metabolism in microorganisms.

O’Donovan¹,

Neuhard²

1970

Bacteriological Reviews

304

180

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Section: Biosynthesis Of Dttpmentioning

confidence: 99%

Pyrimidine metabolism in microorganisms.

O’Donovan¹,

Neuhard²

1970

Bacteriological Reviews

304

180

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“…Transforming DNA was prepared from stationary-phase cultures of donor strains by es- ' Part of a thesis submitted to Yale University in partial fulfillment of the requirements for the Ph.D. degree. 2 Present address: Department of Biochemistry, Duke University Medical Center, Durham, N.C. 27706. sentially the procedure of Marmur (3), adapted as follows. After lysis, Pronase (Calbiochem) was added to about 500 Mg/ml before incubation for 2 hr at 37 C, and the ribonuclease and isopropanol steps were omitted.…”

mentioning

confidence: 99%

Early Intermediate State of Transforming Deoxyribonucleic Acid During Uptake by Bacillus subtilis

Morrison

1971

J Bacteriol

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Examination of the effects of ethylenediaminetetraacetic acid (EDTA) on uptake of transforming deoxyribonucleic acid (DNA) by Bacillus subtilis allowed definition of a new intermediate state of transforming DNA during uptake. Markers in this state, termed "EDTA-resistant," are bound to the cell, are sensitive to inactivation by deoxyribonuclease, but may become deoxyribonuclease-resistant despite the addition of excess EDTA. Markers become EDTA-resistant quadratically with time after the addition of DNA to a competent culture, but linearly after exposure of competent cells to a brief pulse of DNA. An attractive model consistent with these findings is that EDTA blocks only the initiation of entry of a molecule of DNA.

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“…polA), parent strain 168 (thy, tryp) from N. Brown (10), and HA101 (59)F (his, leu. met, polA) from B. Strauss (4) were grown in medium C+ for toluenization experiments and in 1.75% Penassay broth (Difco antibiotic medium 3) supplemented with the amino acid requirement (50 mg/liter) and the base requirement (50 mg/liter) for DNA polymerase preparations. Medium C+ is medium C salts (Spizizen minimal salts medium) (1) containing (per liter): K2HPO4, 14.0 g; KH2PO4, 6.0 g; (NH4)2SO4, 2.0 g; CsH.Na807.2 H20, 1 g; and MgSO4 7 H2O, 0.2 g; and supplemented with glucose, 5 g; Casamino Acids (Difco), 500 mg; amino acid requirement, 50 mg; and base requirement 10 mg.…”

Section: Methodsmentioning

confidence: 99%

Tests for Deoxyribonucleic Acid Synthesis in Toluenized Bacillus subtilis Cells

Matsushita

Sueoka

1974

J Bacteriol

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Several tests were devised to further characterize deoxyribonucleic acid (DNA) synthesis in toluenized Bacillus subtilis cells. Vigorous agitation of toluenized cells (localization test) demonstrated that the DNA replication is exclusively a cell-associated process. A DNA "repair" condition was also applied to toluenized cells and shown to be distinct from DNA replication in its DNA polymerase I dependency and its ability to synthesize DNA on template which is either cell associated or free, outside the cell. This repair condition was used in conjunction with the localization test to demonstrate the penetration of deoxyribonuclease I and possibly DNA polymerase I into toluenized cells. Therefore, we suggest that the localization test can be used to test the penetration of proteins into toluenized cells for both the DNA repair and replication processes.

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Transformable Thymine-Requiring Mutant of Bacillus subtilis

Cited by 147 publications

References 0 publications

Pyrimidine metabolism in microorganisms.

Pyrimidine metabolism in microorganisms.

Early Intermediate State of Transforming Deoxyribonucleic Acid During Uptake by Bacillus subtilis

Tests for Deoxyribonucleic Acid Synthesis in Toluenized Bacillus subtilis Cells

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