Transformation-Associated Recombination (TAR) Cloning of Tumor-Inducing Xmrk2 Gene from Xiphophorus maculatus

Kirchner, Jakob M.; Ivanova, Vesselina; Samson, Autum; Noskov, Vladimir N.; Volff, Jean‐Nicolas; Resnick, Michael A.; Walter, Ronald B.

doi:10.1007/s1012601-0039-9

Cited by 3 publications

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References 37 publications

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“…Within Xiphophorus, recent expansion of genomic techniques has resulted in the cloning of the entire genome in bacterial artificial chromosomes (BACs) 40 as well as of a targeted region using yeast artificial chromosomes (YACs). 52 Thus, estimates of physical distance derived from recombination data should be directly testable in the near future. All of these fish taxa have genome sizes substantially smaller than that of mammals, including human, and the tetraodontiform fishes, including Fugu particularly, has been exploited for the study of comparative genomics.…”

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confidence: 99%

The Genetic Map ofXiphophorusFishes Represented by 24 Multipoint Linkage Groups

et al. 2004

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Hybrids between distinct Xiphophorus species have been utilized for over 70 years to study melanoma and other neoplasms that can develop spontaneously in hybrid offspring. Genetic linkage mapping has proven to be important in delineating genomic areas that harbor oncogenes and tumor suppressors. Within this report, two parallel backcrosses have been utilized to generate a genetic linkage map for Xiphophorus fishes. Isozyme/allozyme, RFLP and PCR-based mapping techniques, including AP-PCR/RAPDs and microsatellite loci were utilized. The derived linkage map provides a total of 403 mapped polymorphisms distributed among 24 linkage groups, representative of 24 acro- and telocentric chromosome pairs. Genomic coverage is approximately one marker per 5.8 cM. Detailed genotypic analysis of the utilized hybrids revealed two areas of the genome that show significant segregation distortion. Loci within the linkage group harboring the sex determining locus (LG 24) and an autosomal linkage group (LG 21) show highly significant deviations from Mendelian expectations. This phenomenon is not present in a hybrid cross that utilizes a different backcross hybrid progenitor species. The derived map with sequence-tagged markers provides a framework for physical map generation, large-scale genomic sequencing and will further enable cross-genome comparisons of vertebrate genomes.

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Section: Discussionmentioning

confidence: 99%

The Genetic Map ofXiphophorusFishes Represented by 24 Multipoint Linkage Groups

et al. 2004

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Xiphophorus

2006

Zebrafish

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A Microsatellite Genetic Linkage Map for XiphophorusSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY258640, AY258896.

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Interspecies hybrids between distinct species of the genus Xiphophorus are often used in varied research investigations to identify genomic regions associated with the inheritance of complex traits. There are 24 described Xiphophorus species and a greater number of pedigreed strains; thus, the number of potential interspecies hybrid cross combinations is quite large. Previously, select Xiphophorus experimental crosses have been shown to exhibit differing characteristics between parental species and among the hybrid fishes derived from crossing them, such as widely differing susceptibilities to chemical or physical agents. For instance, genomic regions harboring tumor suppressor and oncogenes have been identified via linkage association of these loci with a small set of established genetic markers. The power of this experimental strategy is related to the number of genetic markers available in the Xiphophorus interspecies cross of interest. Thus, we have undertaken the task of expanding the suite of easily scored markers by characterization of Xiphophorus microsatellite sequences. Using a cross between Xiphophorus maculatus and X. andersi, we report a linkage map predominantly composed of microsatellite markers. All 24 acrocentric chromosome sets of Xiphophorus are represented in the assembled linkage map with an average intergenomic distance of 7.5 cM. Since both male and female F 1 hybrids were used to produce backcross progeny, these recombination rates were compared between "male" and "female" maps. Although several genomic regions exhibit differences in map length, male-and female-derived maps are similar. Thus Xiphophorus, in contrast to zebrafish, Danio rerio, and several other vertebrate species, does not show sex-specific differences in recombination. The microsatellite markers we report can be easily adapted to any Xiphophorus interspecies and some intraspecies crosses, and thus provide a means to directly compare results derived from independent experiments.

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Transformation-Associated Recombination (TAR) Cloning of Tumor-Inducing Xmrk2 Gene from Xiphophorus maculatus

Cited by 3 publications

References 37 publications

The Genetic Map ofXiphophorusFishes Represented by 24 Multipoint Linkage Groups

The Genetic Map ofXiphophorusFishes Represented by 24 Multipoint Linkage Groups

Xiphophorus

A Microsatellite Genetic Linkage Map for XiphophorusSequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY258640, AY258896.

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