Transformation in Oilseed Rape (Brassica napus L.)

Thomzik, J. E.

doi:10.1007/978-3-642-78037-0_13

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…Some of these compounds also possess promising therapeutic activity in various cancers, including breast, prostate, and neuroblastoma (Aggarwal et al 2004;Laux and Aregullin 2004;King et al 2005;Baur and Sinclair 2006). Direct evidence of resveratrol's biological activity (Adrian et al 1997) and a clear relationship between its production and disease resistance have been demonstrated using transgenic tobacco (Hain et al 1993), oil-seed (Thomzik 1993), tomato Giovinazzo et al 2005;Nicoletti et al 2007), rice (Stark-Lorenzen et al 1997), wheat (Leckband and Lörz 1998), kiwi (Kobayashi et al 2000), alfalfa (Hipskind and Paiva 2000), apple (Szankowski et al 2003), papaya (Zhu et al 2004), banana (Vishnevetsky et al 2005), pea (Richter et al 2006), hop (Schwekendiek et al 2007) and grapevine (Fan et al 2008). On the other hand, little is known about cis-acting elements and transcription factors controlling expression of stilbene synthase in response to various environmental stimuli.…”

Section: Introductionmentioning

confidence: 96%

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Ding

et al. 2009

Planta

127

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Stilbene synthase is a plant-specific polyketide synthase, and plays important roles in diverse metabolic processes. The genomic stilbene synthase gene was cloned from accession "Baihe-35-1" of Chinese wild Vitis pseudoreticulata, and a stilbene synthase of V. pseudoreticulata (VpSTS) transcripts expressed in the grape-powdery mildew interaction were determined by semi-quantitative RT-PCR. To monitor VpSTS expression in plant, the promoter region flanking the 5' VpSTS coding region was isolated from the genomic DNA of Chinese wild V. pseudoreticulata accession Baihe-35-1. Alignment of the VpSTS promoter sequence showed a 56.4% identity to Vitis vinifera. To identify the upstream region of the VpSTS gene required for promoter activity, a series of VpSTS promoter deletion derivatives was constructed. Each deletion construct was analyzed by Agrobacterium-mediated transient transformation in grapevine and tobacco leaves after infection by Uncinula necator and Alternaria alternata. In transiently transformed grapevine leaves, GUS activity was also determined after treatment with salicylic acid (SA) and 4 degrees C cold. Analysis of a series of 5' deletions of the VpSTS promoter in grapevine leaves indicated that the proximal 162 bp from the transcription initiation site was proved to be necessary for establishing both the constitutive and induced pattern of expression.

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Section: Introductionmentioning

confidence: 96%

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Ding

et al. 2009

Planta

127

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“…This was the first report of increased disease resistance in transgenic plants based on added foreign phytoalexins. These first transformation and regeneration experiments in tobacco were followed by others on dicots, such as oilseed rape (Thomzik, 1993), tomato (Thomzik, 1995; in all cases showing an increased resistance to pathogens in plants transformed with grapevine stsy genes. Starck-Lorenzen et al (1997) first transformed these genes into a monocot, rice (Oryza sativa).…”

Section: Transfer Of Stsy Genesmentioning

confidence: 97%

Physiological Role and Molecular Aspects of Grapevine Stilbenic Compounds

Bavaresco

Fregoni

2001

Molecular Biology &Amp; Biotechnology of the Grapevine

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“…Kanamycin was found to interfere with the regeneration of transformed cells of other Brassica species (Pua et al, 1987;Trail et al, 1989;Berthomieu and Jouanin, 1992;Thomzik, 1993) and its introduction had to be delayed for 1±2 wk to regenerate transformants (Srivastava et al 1988;Toriyama et al, 1991;Eimert and Siegemund, 1992;Ovesna Â et al, 1993;Metz et al, 1995). In more extreme cases, both Berthomieu et al (1994) and Passele Águe and Kerlan (1995) did not apply any selection during regeneration of transgenic cabbage and cauliflower plants, which led to the regeneration of very few chimeric transgenic plants along with a large number of nontransformed plants.…”

Section: Discussionmentioning

confidence: 99%

Development of transgenic cabbage (Brassica oleracea var. Capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation

Jin

Liu

Tabashnik

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Transgenic head cabbage (Brassica oleracea var. capitata), resistant to diamondback moth (Plutella xylostella) larvae, was developed through Agrobacterium tumefaciens-mediated transformation with Bacillus thuringiensis (Bt) cry genes using a modified procedure. Factors important for transformation included cabbage cultivar; preculture and coculture of explants on a callus initiation medium; use of appropriate amount; and delay in initial application of selective agents. A total of 15 independent transformed lines with over 100 plants were obtained from several transformation experiments, representing an overall transformation efficiency of ,1%. Cabbage plants transformed with a synthetic Bt gene, cry1Ab3, were all resistant to larvae of the diamondback moth, whereas all plants transgenic for cry1Ia3, a wild-type Bt gene, were susceptible. As a first step towards testing the hypothesis that reduced exposure of Bt to target insects would delay the evolution of insect resistance to Bt, cry1Ab3 expression was put under the transcriptional control of the soybean woundinducible vspB promoter and transgenic cabbage was obtained. Insect bioassay showed that such plants were all resistant to diamondback moth even without induction for the expression of Bt.

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Transformation in Oilseed Rape (Brassica napus L.)

Cited by 12 publications

References 49 publications

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Characterization of a novel stilbene synthase promoter involved in pathogen- and stress-inducible expression from Chinese wild Vitis pseudoreticulata

Physiological Role and Molecular Aspects of Grapevine Stilbenic Compounds

Development of transgenic cabbage (Brassica oleracea var. Capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation

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