Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Gordon‐Kamm, William; Spencer, Tim; Mangano, M. L.; Adams, Thomas R.; Daines, R. J.; Start, W. G.; O'Brien, Jennifer; Chambers, S. A.; Adams, W. R.; Willetts, N. G.; Rice, Thomas B.; Mackey, Catherine J.; Krueger, Roger W.; Kausch, Albert P.; Lemaux, Peggy G.

doi:10.1105/tpc.2.7.603

Cited by 659 publications

(43 citation statements)

References 30 publications

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“…Type II corn callus AT824 was initiated (Gordon-Kamm et al, 1990) and cryopreserved and reinitiated as described in (Aves K, 1992). AT824 callus suspension was maintained on N6 media at 28 °C as described (Aves K, 1992; Gordon-Kamm et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

et al. 2014

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Summary Unlike nuclear multisubunit RNA polymerases I, II and III, whose subunit compositions are conserved throughout eukaryotes, plant RNA Polymerases IV and V are non-essential, Pol II-related enzymes whose subunit compositions are still evolving. Whereas Arabidopsis Pols IV and V differ from Pol II in four or five of their twelve subunits, respectively, and differ from one another in three subunits, proteomic analyses show that maize Pols IV and V differ from Pol II in six subunits, but differ from each other only in their largest subunits. Use of alternative catalytic second-subunits, which are non-redundant for development and paramutation, yields at least two subtypes of Pol IV, and three subtypes of Pol V in maize. Pol IV/V associations with MOP1, RMR1, AGO121, Zm_DRD1/CHR127, SHH2a and SHH2b extend parallels between paramutation in maize and the RNA-directed DNA methylation pathway in Arabidopsis.

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Section: Methodsmentioning

confidence: 99%

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

et al. 2014

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“…At such moments transformed cells have the opportunity to act independently from neighbouring cells, otherwise they have to divide in commitment with untransformed cells. In indirect embryogenesis very young embryos are subjected to secondary embryogenesis and this explains the successful application of indirect embryogenesis for plant transformation in Apium graveolens (Catlin et al, 1988), Beta vulgaris (D' Halluin et al, 1992), Citrus sinensis (Hikado et al, 1982), C. reticulata (Hidaka et al, 1990), Daucus carota (Scott & Draper, 1987), Helianthus annuus (Everett et al, 1987), Medicago sativa (Pezzotti et al, 1991), Oryza sativa (Christou et al, 1991) and Zea mays (Gordon-Kamm et al, 1990).…”

Section: Plant Transformationmentioning

confidence: 99%

Secondary somatic embryogenesis and applications in plant breeding

Raemakers¹,

Jacobsen²,

Visser³

1995

Euphytica

160

107

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Secondary somatic embryogenesis is the phenomenon whereby new somatic embryos are initiated from somatic embryos. Such cultures have been described in at least 80 Gymnosperm and Angiosperm species. In the initial step (primary somatic embryogenesis) such cultures have to be started from plant explants. In general, primary somatic embryogenesis from vegetative plant explants is, indirect and mostly driven by auxin (AUX) or auxin and cytokinin (AUX/CYT) supplemented media, whereas, from zygotic embryos it is direct and driven, to a larger extent, by CYT or growth regulator free media. Primary somatic embryogenesis from floral plant explants is between these two extremes. Indirect and direct somatic embryogenesis should be seen as two extremes of one continuum: in indirect somatic embryogenesis the embryos develop up to the (pre)-globular stage and in direct somatic embryogenesis to mature stages before they are subjected to secondary embryogenesis. In general, secondary embryogenesis requires no growth regulators in species with CYT driven primary embryogenesis. Whereas, continuous exposure to growth regulators is needed in species with CYT/AUX or AUX driven primary embryogenesis.In most species somatic embryos can be converted into shoots, although the frequencies are mostly low. In general, somatic embryos induced by growth regulator free or CYT supplemented media meet more difficulties in shoot development than embryos induced by AUX supplemented media. Applications of secondary somatic embryogenesis for plant breeding are discussed.

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“…O emprego de embriões imaturos de milho para indução de embriogênese somática e regeneração de plantas de milho submetidas à transformação por biobalística é frequente na literatura (GORDON-KAMM et al, 1990;SONGSTAD et al, 1996;BRETTSCHNEIDER et al, 1997;ZHANG et al, 2012). De acordo com SONGSTAD et al (1996), esse tipo de explante permite a obtenção de plantas transgênicas com elevada fertilidade em comparação a outros tipos de explantes.…”

Section: Resultsunclassified

Regeneração de plantas a partir de dois tipos de explantes de milho submetidos à transformação genética por biobalística

Vidigal

Schuster

Texeira³

et al. 2014

Cienc. Rural

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O uso da biobalística na transformação genética de plantas requer a otimização de diversos parâmetros, entre eles, o desenvolvimento de protocolos para regeneração de plantas a partir de células transformadas. Este trabalho teve como objetivo avaliar a eficiência de regeneração de plantas de milho a partir de calos embriogênicos e de explantes sementes divididas, submetidos à transformação por biobalística, utilizando o gene bar como marcador de seleção. Foram submetidos 2000 agrupamentos de calos embriogênicos de milho e 2000 explantes sementes divididas à transformação por biobalística. A seleção dos eventos de transformação foi realizada em meios de cultivo suplementados com glifosinato de amônio. As plântulas selecionadas foram aclimatadas e transplantadas para vasos em casa de vegetação. Com a utilização de embriogênese somática, a eficiência de regeneração foi de 0,2%, enquanto que, com a utilização de sementes divididas, a eficiência de regeneração foi de 1,3%. Embora ambos os protocolos possibilitem a regeneração de plantas de milho submetidas à transformação por biobalística, há a necessidade de aumentar sua eficiência.

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Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Cited by 659 publications

References 30 publications

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

Functional Diversification of Maize RNA Polymerase IV and V Subtypes via Alternative Catalytic Subunits

Secondary somatic embryogenesis and applications in plant breeding

Regeneração de plantas a partir de dois tipos de explantes de milho submetidos à transformação genética por biobalística

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