Transformation of mononuclear dinitrosyl iron complex (DNIC) with thiourea in glutathione aqueous solution

Albumin as a prospective carrier of the nitrosyl iron complex with thiourea and thiosulfate ligands under aerobic conditions

“…As for the previously studied DNICs, we assume an equilibrium: complex 1 ↔ product + NO. 35,41,45 The introduction of Hb into the solution as a “trap” for NO shifts the equilibrium and results in more efficient NO release by complex 1 (Fig. 2(b)) compared to the aerobic and anaerobic sensor electrode data obtained previously.…”

“…S2(a) †). 41 But in the presence of oxygen, their products also absorb in the indicated range (Fig. S2(c) †).…”

A nitrosyl iron complex with 3.4-dichlorothiophenolyl ligands: synthesis, structures and its reactions with targets – carriers of nitrogen oxide (NO) in vivo

“…S10†) and compared with experimental data. The process of replacing the initial ligands of the complexes with glutathione was repeatedly described by us for other complexes; 34,50 however, in this case, there are some features. We consider the starting compound to be complex 3 , which is formed, as shown above, upon dissolution of complex 1 in DMSO.…”

“…82 Our previous studies on NICs with thiourea derivatives and aliphatic thiolyls demonstrated the ability of GSH to replace thiolate ligands with the formation of a binuclear complex with two glutathione ligands [Fe 2 (NO) 4 (GS − ) 2 ] 2+ . 34,50,83 In the case of thiophenol ligands, we assumed that the thioligands were replaced with water and glutathione. 53 In this study, as before, the spectra in the experimental mixture of the complex…”

“…32 Previously, it was shown that NICs can exchange their ligands for glutathione, cysteine, and other thiols, including protein ones, to form more stable NICs. 33,34 It can be assumed that the cytostatic action of some members of this class of compounds is due to their ability to bind free GSH in tumor cells since some modern approaches to chemotherapy are aimed at reducing the level of GSH in cells. 35,36 Therefore, the study of the reaction of NICs with GSH requires careful consideration since the binding of free glutathione will lead to a decrease in its level in cells, which can affect cellular metabolism.…”