Transformation of Perilla frutescens var. crispa Using an Agrobacterium-Ri Binary Vector System.

Yamazaki, Mami; Kobayashi, Mii; Saito, Kazuki

doi:10.5511/plantbiotechnology.14.169

Cited by 6 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concept of using Ri-T-DNA as a vector for regenerating transgenic plants from hairy root cultures has already been in use in a number of plant species due to the fact that roots do not produce chimeras or somaclonal variants (Ooms et al 1985;Tepfer 1984;Ottaviani et al 1990;Yamazaki et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Saxena

Banerjee

Laiq-ur-Rahman³

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Transgenic plants of rose-scented geranium (Pelargonium graveolens cv. Hemanti) have been produced from Agrobacterium rhizogenes (strains A 4 and LBA9402) mediated hairy root cultures. Amongst the explants tested, leaves were most responsive followed by the petioles and internodal segments, respectively. The A 4 strain performed better for all the three explants both in terms of frequency of response and time requirement for hairy root induction. Transgenic shoots could be obtained by spontaneous regeneration without intervening callus phase amongst 16% and 12% root lines of A 4 and LBA 9402 origin, respectively, or they were induced in 29% and 22% hairy root lines of A 4 and LBA9402 origin, respectively, with different hormonal supplementation. These transgenic plants showed 30% survival as against 90% of their control under the confined environment of glasshouse. The transgenic plants were of similar morphotype having increased branching, higher number of leaves with increased dentations, short and round stature, highly branched root system and absence of leaf wrinkling. These transgenic plants showed opine positive results even after 5 months of their transfer to the glasshouse. The essential oil compositions of 81% of these transgenics were qualitatively similar to that of the wild type parent. However, two transgenic plants showed increase in concentrations of geraniol and geranyl esters signifying improved oil quality with respect to the citronellol:geraniol ratio. These two oils having better olfactory value represent an improvement over that of the wild type parent from the commercial point of view.

show abstract

Section: Introductionmentioning

confidence: 99%

Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Saxena

Banerjee

Laiq-ur-Rahman³

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Recovery of Perillaldehyde from Hydrodistillation Condensate ofPerilla frutescens(L.) Britton var.crispaF.viridisLeaves Using Macroporous Resin Adsorption

Chang

Han

Dong

et al. 2013

Separation Science and Technology

View full text Add to dashboard Cite

Molecular Biological Studies on Diversity of Secondary Metabolism in Medicinal Plants and Application to the Production in Transgenic Plants

Yamazaki

2002

YAKUGAKU ZASSHI

View full text Add to dashboard Cite

A molecular biological approach was applied to the study of diversity and regulation of secondary metabolism in medicinal plants at various levels. For the inter-species diversity, RFLP (restriction fragment length polymorphysm) and RAPD (random ampliˆed polymorphic DNA) analyses of genomic DNA were performed on the plants, belonging to the same genus or family and containing related compounds. Phylogenetic trees of lupin alkaloid containing plants and other medicinal plants, based on RFLP and/or RAPD proˆles, showed the relationship between the diversities in genomes and secondary metabolisms. The chemotypes regarding anthocyanin production in Perilla frutescens var. crispa, were subjected to the study on intra-species diversity. The structural genes and the regulatory genes involved in anthocyanin biosynthesis were isolated and their expression in red and green forms was determined by Northern blot analysis. The expression of all structural genes examined was co-ordinately regulated in form-speciˆc manner and by light illumination. The anthocyanin production was enhanced in transgenic plants over-expressing Myc homologue genes from perilla. These results suggested that a protein complex including bHLH factors might regulate the expression of a series of structural genes. Additionally, cDNAs coding anthocyanin 5 O glucosyltransferase and anthocyanidin synthase were isolated and characterized using recombinant proteins for theˆrst time. In conclusion, it was indicated that the molecular biological techniques are powerful tools for the investigation of diversity and regulation of and for the genetic engineering of secondary metabolism in medicinal plants.

show abstract

Transformation of Perilla frutescens var. crispa Using an Agrobacterium-Ri Binary Vector System.

Cited by 6 publications

References 10 publications

Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Recovery of Perillaldehyde from Hydrodistillation Condensate ofPerilla frutescens(L.) Britton var.crispaF.viridisLeaves Using Macroporous Resin Adsorption

Molecular Biological Studies on Diversity of Secondary Metabolism in Medicinal Plants and Application to the Production in Transgenic Plants

Contact Info

Product

Resources

About