Transformation of Sugarbeet (Beta vulgaris L.) and Evaluation of Herbicide Resistance in Transgenic Plants

D’Halluin, Kathleen; Bossut, Martien; Bonne, Els; Mazur, Barbara J.; Leemans, J.; Botterman, Johan

doi:10.1038/nbt0392-309

Cited by 92 publications

(38 citation statements)

References 29 publications

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“…Effective regeneration pathways have been exploited for the large-scale production of transformed sugar beet plants (D'Halluin et al, 1992;Hall et al, 1996;Zhang et al, 2004). The guard cell protoplast system for high efficiency generation of transgenic plants has now been utilised in several academic and commercial laboratories (Hall et al, 1996).…”

Section: Development Of Transgenic Technologymentioning

confidence: 99%

“…IvicHaymes & Smigocki (2005) have developed a direct transformation protocol by microprojectile bombardment. Several Agrobacterium tumefaciens-mediated transformation procedures have been widely applied because of the fact that more transformants with simple integration patterns and desired transgene expression levels were produced (Fry et al, 1991;D'Halluin et al, 1992;Zhang et al, , 2001.…”

Section: Development Of Transgenic Technologymentioning

confidence: 99%

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Genetic approaches to sustainable pest management in sugar beet (Beta vulgaris)

Zhang

Jiang

et al. 2008

Annals of Applied Biology

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Sugar beet (Beta vulgaris) is an important arable crop, traditionally used for sugar extraction, but more recently, for biofuel production. A wide range of pests, including beet cyst nematode (Heterodera schachtii), root-knot nematodes (Meloidogyne spp.), green peach aphids (Myzus persicae) and beet root maggot (Tetanops myopaeformis), infest the roots or leaves of sugar beet, which leads to yield loss directly or through transmission of beet pathogens such as viruses. Conventional pest control approaches based on chemical application have led to high economic costs. Development of pest-resistant sugar beet varieties could play an important role towards sustainable crop production while minimising environmental impact. Intensive Beta germplasm screening has been fruitful, and genetic lines resistant to nematodes, aphids and root maggot have been identified and integrated into sugar beet breeding programmes. A small number of genes responding to pest attack have been cloned from sugar beet and wild Beta species. This trend will continue towards a detailed understanding of the molecular mechanism of insect-host plant interactions and host resistance. Molecular biotechnological techniques have shown promise in developing transgenic pest resistance varieties at an accelerated speed with high accuracy. The use of transgenic technology is discussed with regard to biodiversity and food safety.

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Section: Development Of Transgenic Technologymentioning

confidence: 99%

Section: Development Of Transgenic Technologymentioning

confidence: 99%

Genetic approaches to sustainable pest management in sugar beet (Beta vulgaris)

Zhang

Jiang

et al. 2008

Annals of Applied Biology

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“…Tiene una amplia historia de uso como marcador de selección y en cultivos GM tolerantes a este herbicida, incluyendo maíz (Gordom-Kamm et al, 1990), arroz (Christou et al, 1991), trigo (Vasil et al, 1992), remolacha azucarera (Beta vulgaris) (D'Halluin et al, 1992), canola (Brassica napus) y alfalfa (Medicago sativa) (Cobb, 1992), algodón (Gossypium hirsutum) (Keller et al, 1997), lechuga (Lactuca sativa) (Mohapatra et al, 1999), caña de azúcar (Saccharum officinarum) (Falco et al, 2000), yuca (Manihot esculenta) (Sarria et al, 2000) y fríjol (Phaseolus vulgaris) (Aragão et al, 2002). En el mundo los cultivos comerciales aprobados, tolerantes a glufosinato incluyen remolacha azucarera, canola, soya, arroz y maíz.…”

Section: Discusión Genes De Tolerancia a Sequía Y Glufosinato De Amonunclassified

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

Carreño-Venegas

Chaparro-Giraldo

2016

Acta biol. Colomb.

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RESUMENComo primera aproximación en la obtención de una línea transgénica de maíz tolerante a sequía y al herbicida glufosinato de amonio, se seleccionaron genes y elementos reguladores para el diseño in silico de casetes de expresión, a través del análisis de literatura científica y bases de datos de genes y patentes. Las secuencias génicas fueron modificadas con base en el criterio de uso codónico del maíz para optimizar su expresión. Los casetes de expresión diseñados con el software DNA 2.0., fueron sintetizados por una empresa especializada. La presencia del transgen y la expresión a nivel de mARN fue demostrada mediante PCR y RT-PCR en la planta modelo Nicotiana benthamiana transformada vía Agrobacterium tumefaciens. Un ensayo preliminar in vitro en condiciones simuladas de sequía en medio MS con PEG (PM 6000)10 % no demostró incremento notorio en la tolerancia de las plántulas transformantes, posiblemente debido a que el uso codónico del diseño no favorece la expresión génica en la planta modelo.Palabras clave: estrés abiótico, tolerancia a herbicida, transgénesis, uso codónico. ABSTRACTAs a first approach in obtaining a transgenic drought and glufosinate ammonium tolerant maize line, genes and regulatory elements for the in silico design of the expression cassettes were selected through analysis of scientific literature and databases of genes and patents. Gene sequences were modified based on the criterion of maize codon usage to optimize their expression. The constructs designed with DNA 2.0 Software, were synthesized by a specialized company. The presence of the transgene and the expression of mRNA was demonstrated by PCR and RT-PCR in the model plant Nicotiana benthamiana transformed via Agrobacterium tumefaciens. A preliminary experiment in vitro under simulated drought conditions in MS medium with 10 % PEG (PM 6000) showed no noticeable increase in drought tolerance of the transformants, possibly because the codon usage of the design does not promote gene expression in the model plant.

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“…Agrobacterium tumefaciens-mediated transformation (Lindsey and Gallois, 1990;D'Halluin et al, 1992aD'Halluin et al, , 1992bKrens et al, 1996;Snyder et al, 1999;Hisano et al, 2004), particle bombardment-mediated transformation (Snyder et al, 1999), and protoplast-based transformation of sugar beet have already been described in the past. In general, Agrobacteriummediated transformation is simpler, more efficient, and less expensive compared to other systems and also results in a low copy number of insertions.…”

Section: Introductionmentioning

confidence: 99%

Generation of transgenic sugar beet (Beta vulgarism L.) overexpressing the polygalacturonase inhibiting protein 1 of Phaseolus vulgaris (PvPGIP1) through Agrobacterium-mediated transformation

et al. 2015

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Numerous diseases caused by fungal pathogens influence the annual production of sugar beet. In order to obtain a plant resistant to fungi, genetic transformation has been applied to the sugar beet. To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes (CWDEs); polygalacturonases (PGs) are pathogenicity factors produced at the earlier stages of a fungal infection that depolymerize the homogalacturonan. One of the strategies used by plants to limit the degradation of the cell wall polysaccharides by fungal CWDEs is the production of proteinaceous inhibitors. Against fungal, microbial, and insect PGs, plants produce cell wall-associated polygalacturonase-inhibiting proteins (PGIPs). The overexpression of PGIPs improves the resistance to fungal and bacterial necrotrophs in different plants. In this research, the gene encoding the PGIP1 fused downstream of the leader sequence for secretion in the extracellular environment was isolated from Phaseolus vulgaris and cloned into the expression vector pBI121 for the Agrobacterium-mediated transformation of sugar beet. Modified transformation protocol and selection strategies were developed. In comparison with the preexisting methods, the transformation efficiency was increased and different cultivars were transformed, highlighting the general effectiveness of the method applied. The presence of the transgene and the activity of PvPGIP1 were confirmed by PCR and agarose diffusion assay analyses, respectively, and the present and copy number of the transgene in the T0 plants' genome were demonstrated by Southern blot.

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Transformation of Sugarbeet (Beta vulgaris L.) and Evaluation of Herbicide Resistance in Transgenic Plants

Cited by 92 publications

References 29 publications

Genetic approaches to sustainable pest management in sugar beet (Beta vulgaris)

Genetic approaches to sustainable pest management in sugar beet (Beta vulgaris)

Diseño de casetes de expresión que confieran tolerancia a sequía y a glufosinato en maíz (Zea mays)

Generation of transgenic sugar beet (Beta vulgarism L.) overexpressing the polygalacturonase inhibiting protein 1 of Phaseolus vulgaris (PvPGIP1) through Agrobacterium-mediated transformation

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