Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Abdel-Razik, A. B.; Hammad, Ibtisam; Tawfik, Eman

doi:10.9790/264x-03030113

Cited by 21 publications

(11 citation statements)

References 26 publications

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“…The CSNPs/pDNA retention and release results agreed with those of Abdel‐Razik et al [12], who used CSNPs to deliver the thionine gene. Formation of the CSNPs/pDNA complex occurs because of the electrostatic interactions between the positively charged amino groups (glucosamine units) of the CSNPs and the negatively charged phosphates of the DNA backbone [11].…”

Section: Discussionsupporting

confidence: 87%

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Recombinant overexpression of the Escherichia coli acetyl‐CoA carboxylase gene in Synechocystis sp. boosts lipid production

et al. 2021

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Microalgae have received continued attention as a potential source for biofuel production. However, the lack of suitable strains that provide a lipid‐rich biomass and tolerate harsh condition inhibits their industrial application. This report describes an effort to transform Synechocystis sp. with genes encoding acetyl‐CoA carboxylase (ACC), a key regulatory enzyme in the lipogenesis pathway, from the white mustard plant (Sinapis alba) and the bacterium Escherichia coli DH5α using chitosan nanoparticles. Although a recombinant plasmid encoding S. alba ACC failed to express, successful transformation was achieved with a recombinant plasmid encoding E. coli DH5α ACC. The successful transformant, Synechocystis sp. PAK13, exhibited increased ACC expression compared with its wild‐type parent (11.8 vs. 7.2 ng), which significantly increased its lipid content (by 3.6‐fold). Synechocystis sp. PAK13 also exhibited a significant (20%) reduction in photosynthetic pigments, a 1.52‐fold higher glucose content and a 3.5‐fold lower sucrose content than the wild‐type. In conclusion, this report introduces a useful strategy to overexpress the ACC gene in microalgae, creating strains with improved lipid production that are suited to industrial applications.

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Section: Discussionsupporting

confidence: 87%

“…The availability of functional amino groups in the chitosan structure facilitates an electrostatic interaction between CSNPs and negatively charged plasmid DNA (pDNA) [11]. CSNP/pDNA complexes show high stability, which prevents DNA degradation before introduction into the cellular cytosol [12].…”

Section: Introductionmentioning

confidence: 99%

Recombinant overexpression of the Escherichia coli acetyl‐CoA carboxylase gene in Synechocystis sp. boosts lipid production

et al. 2021

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“…The thionin gene is genetically modelled in the Arabidopsis thaliana plant according to previous study of Abdel-Razik et al [19]. Onion cultivar was used as a transgenic model (Giza red).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The method of chitosan nanoparticle transformation into plant tissue was developed by [19]: "Seedlings of A. cepa were inoculated by syringe containing CS/pDNA complex. After that the explants were transferred on to MS medium supplemented with (2mg/L BA and 1mg/l kin) hormones and 100µg/L ampicillin then incubated at 25±1°C for 4 weeks to regenerate plants".…”

Section: Transformation Of Chitosan/pdna Into Allium Cepamentioning

confidence: 99%

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Production of transgenic Allium cepa by nanoparticles to resist Aspergillus niger infection

2021

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Transgenic plants are becoming a more powerful tool in modern biotechnology. Genetic engineering was used in biotech-derived products to create genetically modi ed (GM) plants. Plant bioreactor systems have proven to be extremely effective in the production of disease resistance plants. The onion (Allium cepa, L.) is a common, important perennial vegetable crop grown in Egypt for food and economic value. Onions are susceptible to a variety of fungal infections and diseases. Aspergillus niger is a common onion phytopathogen that causes diseases such as black mould (or black rot), which is a major issue, particularly when exporting onions. A.niger grows between the bulb's outer (dead, aky) skin and the rst eshy scales, which become water-soaked. Thionin genes produce thionin proteins, which have antimicrobial properties against a variety of phytopathogens, including A. niger. Chitosan nanoparticles act as a carrier for the thionin gene, which allows A. cepa to resist infection by A. niger. Transgenic A. cepa has a high level of resistance to fungal infection. Transgenic A. cepa had a 27 % weight inhibition compared to non-transgenic one, which had a 69 % inhibition. The expressed thionin protein has a 52 % inhibitory effect on A. niger spore germination. All of these ndings supported thionin protein's antifungal activity as an antimicrobial peptide. Furthermore, the data presented here demonstrated the e cacy of chitosan nanoparticles in gene transformation. The present study describes the bene ts of producing transgenic onion resistance to black rot diseases.positive control; (c) spores treated with transgenic thio-60 protein plant protein.

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Chitosan-Based Nanostructures in Plant Protection Applications

Al-Dhabaan

Mostafa

Almoammar

et al. 2018

Nanotechnology in the Life Sciences

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Transformation of Thionin Genes Using Chitosan Nanoparticle into Potato Plant to Be Resistant to Fungal Infection

Abstract: Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663

Cited by 21 publications

References 26 publications

Recombinant overexpression of the Escherichia coli acetyl‐CoA carboxylase gene in Synechocystis sp. boosts lipid production

Recombinant overexpression of the Escherichia coli acetyl‐CoA carboxylase gene in Synechocystis sp. boosts lipid production

Production of transgenic Allium cepa by nanoparticles to resist Aspergillus niger infection

Chitosan-Based Nanostructures in Plant Protection Applications

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