Transformation of TNT, 2,4-DNT, and PETN by Raoultella planticola M30b and Rhizobium radiobacter M109 and exploration of the associated enzymes

Arbeli, Ziv; Terán, Wilson; Roldán, Fabio

doi:10.1007/s11274-020-02962-8

Cited by 13 publications

(4 citation statements)

References 52 publications

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“…[ 1 , 2 ] 2,4‐DNT is widely used in the production of explosives, as well as in the manufacture of herbicides, plastics, and automobile airbags, resulting in significant environmental pollution and carbon emissions, posing a severe threat to public health. [ 3 , 4 , 5 ] Many published studies have established the carcinogenic effects of 2,4‐DNT on humans [ 6 ] and mutagenic toxicity to microorganisms, algae, fish, and plants. [ 2 , 7 ] Recently, the World Health Organization and the United States Environmental Protection Agency classified 2,4‐DNT as a Group 2B human carcinogen and priority pollutant, respectively [ 8 , 9 ] Consequently, the increasing concern about 2,4‐DNT pollution has sparked a frenzy to eliminate it from the environment.…”

Section: Introductionmentioning

confidence: 99%

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Gao,

Li,

Zhu

et al. 2023

Advanced Science

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Pervasive environmental contamination due to the uncontrolled dispersal of 2,4‐dinitrotoluene (2,4‐DNT) represents a substantial global health risk, demanding urgent intervention for the removal of this detrimental compound from affected sites and the promotion of ecological restoration. Conventional methodologies, however, are energy‐intensive, susceptible to secondary pollution, and may inadvertently increase carbon emissions. In this study, a 2,4‐DNT degradation module is designed, assembled, and validated in rice plants. Consequently, the modified rice plants acquire the ability to counteract the phytotoxicity of 2,4‐DNT. The most significant finding of this study is that these modified rice plants can completely degrade 2,4‐DNT into innocuous substances and subsequently introduce them into the tricarboxylic acid cycle. Further, research reveals that the modified rice plants enable the rapid phytoremediation of 2,4‐DNT‐contaminated soil. This innovative, eco‐friendly phytoremediation approach for dinitrotoluene‐contaminated soil and water demonstrates significant potential across diverse regions, substantially contributing to carbon neutrality and sustainable development objectives by repurposing carbon and energy from organic contaminants.

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Section: Introductionmentioning

confidence: 99%

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Gao,

Li,

Zhu

et al. 2023

Advanced Science

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“…Species such as R. terrigena , R. planticola , R. electrica , R. trevisani , and R. ornithinolytica belong to this genus. R. ornithinolytica is the most important because it has been associated with symptomatic cases of bacteremia ( Kanki et al, 2002 ; Mau and Ross, 2010 ; Haruki et al, 2014 ; Tafoukt et al, 2017 ; Avellaneda et al, 2020 ; Wyres et al, 2020 ), urinary tract infections ( García-Lozano et al, 2013 ; Haruki et al, 2014 ), joint infections ( Beye et al, 2018 ), and biliary tract infections ( Cleveland et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Emergence of Raoultella ornithinolytica in human infections from different hospitals in Ecuador with OXA-48-producing resistance

Villacís,

Castelán-Sánchez,

Rojas-Vargas

et al. 2023

Front. Microbiol.

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PurposeThe purpose of this study was to highlight the clinical and molecular features of 13 Raoultella ornithinolytica strains isolated from clinical environments in Ecuador, and to perform comparative genomics with previously published genomes of Raoultella spp. As Raoultella is primarily found in environmental, clinical settings, we focused our work on identifying mechanisms of resistance that can provide this bacterium an advantage to establish and persist in hospital environments.MethodsWe analyzed 13 strains of Raoultella ornithinolytica isolated from patients with healthcare associated infections (HAI) in three hospitals in Quito and one in Santo Domingo de Los Tsáchilas, Ecuador, between November 2017 and April 2018. These isolates were subjected to phenotypic antimicrobial susceptibility testing, end-point polymerase chain reaction (PCR) to detect the presence of carbapenemases and whole-genome sequencing.ResultsPolymerase chain reaction revealed that seven isolates were positive isolates for blaOXA–48 and one for blaKPC–2 gene. Of the seven strains that presented the blaOXA–48 gene, six harbored it on an IncFII plasmid, one was inserted into the bacterial chromosome. The blaKPC gene was detected in an IncM2/IncR plasmid. From the bioinformatics analysis, nine genomes had the gene blaOXA–48, originating from Ecuador. Moreover, all R. ornithinolytica strains contained the ORN-1 gene, which confers resistance for β-lactams, such as penicillins and cephalosporins. Comparative genome analysis of the strains showed that the pangenome of R. ornithinolytica is considered an open pangenome, with 27.77% of core genes, which could be explained by the fact that the antibiotic resistance genes in the ancestral reconstruction are relatively new, suggesting that this genome is constantly incorporating new genes.ConclusionThese results reveal the genome plasticity of R. ornithinolytica, particularly in acquiring antibiotic-resistance genes. The genomic surveillance and infectious control of these uncommon species are important since they may contribute to the burden of antimicrobial resistance and human health.

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“…To achieve this goal, it is crucial to adopt e cient, economic, and environmentally friendly technologies, such as bioremediation. Despite its recalcitrance and persistence, several in vitro studies have shown that PETN is susceptible to bacterial transformation under aerobic and anaerobic conditions (Binks et al 1996;Zhuang et al 2012;Avellaneda et al 2020). The most detailed studies on aerobic PETN transformation were carried out with Enterobacter cloacae PB2 and Agrobacterium radiobacter (Binks et al 1996; White et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The enzymes that catalyze the transformation of PETN in Enterobacter cloacae PB2 and Agrobacterium radiobacter (PETN reductase and GTN reductase, respectively) belonged to the old yellow enzyme family, which is widespread in bacteria and other organisms (French et al 1996;Snape et al 1997;Williams and Bruce 2002). Indeed, two other PETN transforming bactertia, Raoultella planticola M30b and Rhizobium radiobacter M109, contain genes that share 89.0% identity with PETN reductase and 96.7% identity with the GTN reductase, respectively (Avellaneda et al 2020). Thus, transformation of PETN might be common in bacteria but reports are limited.…”

Section: Introductionmentioning

confidence: 99%

Bacteria isolated from explosive contaminated environments transform pentaerythritol tetranitrate (PETN) under aerobic and anaerobic conditions

Avila-Arias,

Casallas,

Arbeli

et al. 2023

Letters in Applied Microbiology

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Pentaerythritol tetranitrate (PETN) is a nitrate ester explosive that may be persistent, with scarce reports on its environmental fate and impacts. Our main objective was to isolate and characterize bacteria that transform PETN under aerobic and anaerobic conditions. Biotransformation of PETN (100 mg L−1) was evaluated using mineral medium with (M+C) and without (M-C) additional carbon source under aerobic conditions, and with additional carbon source under anaerobic conditions. Here we report on the isolation of 12 PETN transforming cultures (4 pure and 8 co-cultures) from environmental samples collected at an explosive manufacturing plant. The highest transformation of PETN was observed for cultures in M+C under aerobic conditions, reaching up to 91±2% in 2 days. Under this condition, PETN biotransformation was observed in conjunction with the release of nitrites and bacterial growth. No substantial transformation of PETN (<45%) was observed during 21 days in M-C under aerobic conditions. Under anaerobic conditions, five cultures could transform PETN (up to 52±13%) as the sole nitrogen source, concurrent with the formation of two unidentified metabolites. PETN-transforming cultures belonged to Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Actinobacteria. In conclusion, we isolated 12 PETN-transforming cultures belonging to diverse taxa suggesting that PETN transformation is phylogenetically widespread.

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Transformation of TNT, 2,4-DNT, and PETN by Raoultella planticola M30b and Rhizobium radiobacter M109 and exploration of the associated enzymes

Cited by 13 publications

References 52 publications

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Emergence of Raoultella ornithinolytica in human infections from different hospitals in Ecuador with OXA-48-producing resistance

Bacteria isolated from explosive contaminated environments transform pentaerythritol tetranitrate (PETN) under aerobic and anaerobic conditions

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