Transforming Growth Factor  1 Improves Wound Healing and Random Flap Survival in Normal and Irradiated Rats

Nall, Agnes V.; Brownlee, Richard E.; Colvin, Clifford; Schultz, Gregory S.; Fein, D.; Cassisi, Nicholas J.; Nguyen, Tuan Huy; Kalra, Anjay

doi:10.1001/archotol.1996.01890140057011

Cited by 91 publications

(45 citation statements)

References 22 publications

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“…In another study, NALL et al 12 found that tensile strength tended to decrease in irradiated groups as compared to nonirradiated groups after a single 15-Gy dose, but the difference was not significant. Our results for the conventional group matched those of NALL et al 12 and clinical studies 9 ; the tissue strength decreased, but the difference was not significant. This was due to the tissuesparing effects of therapeutic RT in small daily fractions, rather than largefraction doses.…”

Section: Discussionmentioning

confidence: 92%

The effect of pre-operative conventional and hyperfractionated radiotherapy schedules on wound healing and tensile strength in rats: an experimental study

Özbek

Güneren

Yıldız

et al. 2005

International Journal of Oral and Maxillofacial Surgery

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Section: Discussionmentioning

confidence: 92%

The effect of pre-operative conventional and hyperfractionated radiotherapy schedules on wound healing and tensile strength in rats: an experimental study

Özbek

Güneren

Yıldız

et al. 2005

International Journal of Oral and Maxillofacial Surgery

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“…Pre-chinical models have demonstrated the effect of cytokines on healing of both irradiated and nonirradiated wounds [31][32][33][34][35][36][37][38][39]. An explanation for the lack of response seen in Protocol 2 may be that the placement of the fibroblast/fibrin implant into the deep wound of the muscle excision does not position the fibroblasts where they can participate in the healing of the skin.…”

Section: Discussionmentioning

confidence: 99%

Effect of radiation and cell implantation on wound healing in a rat model

Dantzer

Ferguson

Hill

et al. 2003

Journal of Surgical Oncology

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“…Inhibition of bone resorption utilizing bisphosphonates has been shown to reduce bone mineral loss at anterior cruciate ligament entheses after joint injury (65). However, TGF-β, as a crucial growth factor, may be activated by means other than osteoclast bone resorption and plays an important role in a number of processes: inflammation, cell migration, stimulating angiogenesis, epithelial mesenchymal transition (EMT), endothelial mesenchymal transition (EndoMT), and remodeling of the new ECM (66)(67)(68)(69). Therefore, drugs targeting osteoclast resorption may not be as effective as directly targeting TGF-β signaling and would need further preclinical studies to validate.…”

Section: Methodsmentioning

confidence: 99%

Aberrant TGF-β activation in bone tendon insertion induces enthesopathy-like disease

Wang¹,

Xie²,

Crane³

et al. 2018

Journal of Clinical Investigation

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and systemically in spondyloarthropathy patients with enthesopathy (37,38). In this study, we determined the role of TGF-β in Achilles tendon enthesopathy using 3 different mouse models. We found that the changes of calcaneal bone microstructure and composition were associated with the onset of Achilles tendon enthesopathy. Inhibition of TGF-β activity, using either a TGF-β-neutralizing antibody (1D11) or a conditional knockout mouse model, attenuated calcaneal and fibrocartilage pathological changes. ResultsUpregulated TGF-β activities in posterior calcaneal tuberosity in Achilles tendon enthesopathy mouse models. To examine the changes at the disease has drawn increasing attention in recent years. During tissue injury or remodeling, TGF-β is activated to recruit stem/ progenitor cells to maintain tissue homeostasis (27)(28)(29)(30)(31). We have previously shown that TGF-β is released from the bone matrix and activated during osteoclastic bone resorption (31). Active TGF-β induces mesenchymal stromal stem cell (MSC) migration to the bone-resorptive pits for new bone formation (31). However, excessive active TGF-β leads to uncoupled bone remodeling, contributing to the pathogenesis of rare genetic skeletal diseases such as Camurati-Engelmann disease (CED), osteogenesis imperfecta, and more common musculoskeletal disorders, such as osteoarthritis (31-36). High levels of TGF-β have been observed locally lower in relation to sham-operated controls ( Figure 1B). Moreover, a dramatically higher trabecular pattern factor (Tb.Pf), a quantitative parameter of the connectiveness and microarchitecture of trabecular bone (40), was noted in the SMTS mice compared with sham-operated controls, indicating uncoupled bone remodeling ( Figure 1B). Evident bony projections at the PCT were present in 4 of 10 mice 8 weeks after SMTS ( Figure 1A). The BV/TV, Tb.N, and Tb.Th of vertebral bodies were not significantly changed in the SMTS group compared with controls at week 8 (Supplemental Figure 2). Tartrate-resistant acid phosphatase (TRAP) staining showed that the osteoclast surface per bone surface (OCS/BS) increased in PCT trabecular bone 1 and 2 weeks after surgery and was back to normal levels on week 4 after surgery (Figure 1, C and D) while leaving large bone marrow cavities ( Figure 1C). A significantly higher active TGF-β concentration in serum was seen in the SMTS mice relative to sham-operated mice from 1 to 8 weeks after onset of enthesopathy, we generated these 2 models based on prior theories that enthesopathy results from unbalanced mechanical loading (2, 39). The first model we generated decreased mechanical load by partially transecting Achilles tendons in the middle of tendon body in 3-month-old male mice, termed semi-Achilles tendon transection (SMTS) (Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/ JCI96186DS1). The bone volume over total tissue volume (BV/ TV) in the posterior calcaneal tuberosity (PCT), the site of Achilles tendon attachment to calcane...

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Transforming Growth Factor 1 Improves Wound Healing and Random Flap Survival in Normal and Irradiated Rats

Cited by 91 publications

References 22 publications

The effect of pre-operative conventional and hyperfractionated radiotherapy schedules on wound healing and tensile strength in rats: an experimental study

The effect of pre-operative conventional and hyperfractionated radiotherapy schedules on wound healing and tensile strength in rats: an experimental study

Effect of radiation and cell implantation on wound healing in a rat model

Aberrant TGF-β activation in bone tendon insertion induces enthesopathy-like disease

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