Transforming heterotrophic to autotrophic denitrification process: Insights into microbial community, interspecific interaction and nitrogen metabolism

Huang, Xiao; Duan, Chongsen; Yu, Jianguo; Dong, Wenyi

doi:10.1016/j.biortech.2021.126471

Cited by 54 publications

(10 citation statements)

References 39 publications

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“…These data indicate that despite the presence of heterotrophic bacteria in the reactor, the effective denitrification process is taking place mostly by autotrophic denitrifiers that are unable to grow in the test media. It has been recently shown that the shift of the microbial community from heterotrophic to autotrophic denitrification is feasible, but it is rather tedious and needs specific conditions (Huang et al, 2022). Our results also confirm that the autotrophic denitrifying community probably remains stable and is not replaced with heterotrophs during the cycle.…”

Section: Figuresupporting

confidence: 81%

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Lust

Nerut

Gadegaonkar

et al. 2022

Front. Environ. Sci.

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Microbial electrosynthesis is a promising solution for removing nitrate from water with a low concentration of electron donors. Three single-chamber microbial electrosynthesis reactors were constructed and operated for almost 2 years. The single-chamber reactor design saves on construction costs, and the pH of the solute is more stable than that in the case of a two-chamber reactor. Nitrate reduction started at the working electrode potential of −756 mV versus standard hydrogen electrode (SHE), and subsequently, the working electrode potential could be increased without hindering the process. The optimal potential was −656 mV versus SHE, where the highest Faradaic efficiency of 71% and the nitrate removal rate of 3.8 ± 1.2 mgN-NO3/(L×day) were registered. The abundances of nitrite reductase and nitrous oxide reductase genes were significantly higher on the working electrode compared to the counter electrode, indicating that the process was driven by denitrification. Therefore, a microbial electrosynthesis reactor was successfully applied to remove nitrate and can be utilized for purifying water when adding organic compounds as electron donors is not feasible, that is, groundwater. In addition, at the lower working electrode potentials, the dissimilatory nitrate reduction to ammonium was observed.

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Section: Figuresupporting

confidence: 81%

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Lust

Nerut

Gadegaonkar

et al. 2022

Front. Environ. Sci.

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“…Hence, heterotrophic denitrification could effectively remove nitrates, and the higher concentration of NO 3 − –N in the top zone promoted the higher abundance, diversity, and richness of denitrifying bacteria, which led to high denitrification efficiency. 17,18 Besides, the organic electron donor was consumed in the top zone, and the available organic electron donor in the middle and bottom zones was not enough to support the removal of the rest nitrates. However, the ED below the bottom zone made hydrogen escape into the bottom zone.…”

Section: Resultsmentioning

confidence: 99%

Vertical spatial denitrification performance and microbial community composition in denitrification biofilters coupled with water electrolysis

Tang,

Huang,

Tan

et al. 2024

RSC Adv.

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“…Considering that both soils featured a similar abundance of the total bacterial community, this implied a higher proportion of heterotrophs among the beech soil communities. Growth of heterotrophs is favoured by high quantities of simple and complex carbon substrates released as root exudates (Huang et al, 2022;Li et al, 2018;Lladó et al, 2017;Vijay et al, 2019), whereas soils are usually deficient in reduced inorganic compounds (Jones et al, 2018) required as energy sources for autotrophic growth (Brock et al, 2003;Berg, 2011). The redox potential of half reactions utilized by chemolithoautotrophs for energy often leads to lower energy yield than commonly observed for heterotrophs, causing chemolithoautotrophs to grow more slowly (Hooper and DiSpirito, 2013;Madigan et al, 2015;In't Zandt et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Temperature sensitivity of dark CO₂ fixation in temperate forest soils

Akinyede

Taubert²,

Schrumpf³

et al. 2022

Biogeosciences

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Abstract. Globally, soil temperature to 1 m depth is predicted to be up to 4 ∘C warmer by the end of this century, with pronounced effects expected in temperate forest regions. Increased soil temperatures will potentially increase the release of carbon dioxide (CO2) from temperate forest soils, resulting in important positive feedback on climate change. Dark CO2 fixation by microbes can recycle some of the released soil CO2, and CO2 fixation rates are reported to increase under higher temperatures. However, research on the influence of temperature on dark CO2 fixation rates, particularly in comparison to the temperature sensitivity of respiration in soils of temperate forest regions, is missing. To determine the temperature sensitivity (Q10) of dark CO2 fixation and respiration rates, we investigated soil profiles to 1 m depth from beech (deciduous) and spruce (coniferous) forest plots of the Hummelshain forest, Germany. We used 13C-CO2 labelling and incubations of soils at 4 and 14 ∘C to determine CO2 fixation and net soil respiration rates and derived the Q10 values for both processes with depth. The average Q10 for dark CO2 fixation rates normalized to soil dry weight was 2.07 for beech and spruce profiles, and this was lower than the measured average Q10 of net soil respiration rates with ∼2.98. Assuming these Q10 values, we extrapolated that net soil respiration might increase 1.16 times more than CO2 fixation under a projected 4 ∘C warming. In the beech soil, a proportionally larger fraction of the label CO2 was fixed into soil organic carbon than into microbial biomass compared to the spruce soil. This suggests a primarily higher rate of microbial residue formation (i.e. turnover as necromass or release of extracellular products). Despite a similar abundance of the total bacterial community in the beech and spruce soils, the beech soil also had a lower abundance of autotrophs, implying a higher proportion of heterotrophs when compared to the spruce soil; hence this might partly explain the higher rate of microbial residue formation in the beech soil. Furthermore, higher temperatures in general lead to higher microbial residues formed in both soils. Our findings suggest that in temperate forest soils, CO2 fixation might be less responsive to future warming than net soil respiration and could likely recycle less CO2 respired from temperate forest soils in the future than it does now.

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Transforming heterotrophic to autotrophic denitrification process: Insights into microbial community, interspecific interaction and nitrogen metabolism

Cited by 54 publications

References 39 publications

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Vertical spatial denitrification performance and microbial community composition in denitrification biofilters coupled with water electrolysis

Temperature sensitivity of dark CO₂ fixation in temperate forest soils

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Transforming heterotrophic to autotrophic denitrification process: Insights into microbial community, interspecific interaction and nitrogen metabolism

Cited by 54 publications

References 39 publications

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Single-chamber microbial electrosynthesis reactor for nitrate reduction from waters with a low-electron donors’ concentration: from design and set-up to the optimal operating potential

Vertical spatial denitrification performance and microbial community composition in denitrification biofilters coupled with water electrolysis

Temperature sensitivity of dark CO2 fixation in temperate forest soils

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Temperature sensitivity of dark CO₂ fixation in temperate forest soils