Transgene expression enhancement in T-lymphoma cell lines

Ruybal, Paula; Gravisaco, Marí­a José; Barcala, Virna; Escalada, Ana; Cremaschi, Graciela; Taboga, Oscar; Waldner, Claudia; Montagnoli, Claudia

doi:10.1016/j.intimp.2005.04.016

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…The LBC cells express neither progesterone nor estrogen receptors, and hence tumor growth is not affected by medroxyprogesterone or estradiol (33). Therefore, LBC lymphoblastic leukemia constitutes a useful model for the study of human hematological malignancies, similar to the well-known murine T-cell lymphomas EL-4 and BW5147 (34). Tumor cells were cultured in supplemented medium with 50 mol/L 2-␤-mercaptoethanol.…”

Section: Lymphoma Modelmentioning

confidence: 99%

Involvement of Thyroid Hormones in the Alterations of T-Cell Immunity and Tumor Progression Induced by Chronic Stress

Frick

Rapanelli

Bussmann

et al. 2009

Biological Psychiatry

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Section: Lymphoma Modelmentioning

confidence: 99%

Involvement of Thyroid Hormones in the Alterations of T-Cell Immunity and Tumor Progression Induced by Chronic Stress

Frick

Rapanelli

Bussmann

et al. 2009

Biological Psychiatry

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“…Expression of HHV6B U20 in our stable SupT1 cell line was low, likely due to silencing of the CMV promoter. To enhance the expression level of U20, we incubated the U20 SupT1 cells in phorbol myristate acetate (PMA), which has been shown to enhance expression from the CMV promoter (Fig 3)((26–28)). Exogenously-expressed HHV6B U20 mirrored U20 from HHV6B-infected cells, and also migrated at approximately 100 kDa.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the HHV-6 U20 immunoevasin

Schneider

Whyte

Konrad

et al. 2022

Preprint

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Roseoloviruses (HHV-6A, -6B, and -7) infect >90% of the human population during early childhood, and are thought to remain latent or persistent throughout the life of the host. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet very little is known about how these viruses so successfully escape host defenses. Herein, we characterize the HHV6A and HHV6B U20 gene products, which are encoded within a block of genes unique to the roseoloviruses, and therefore of particular interest. Despite 92% amino acid identity, U20 proteins from HHV6A and 6B have been shown to possess different host evasion functions. Here we characterize expression, trafficking, and post-translational modifications of U20 during HHV6A infection. While U20 localized to lysosomes in HHV-6A-infected cells, HHV-6B U20 trafficked to the cell surface and was rapidly internalized. HHV-6B U20 trafficked slowly through the secretory system, receiving several post translational modifications to its N-linked glycans indicative of surface expressed glycoproteins. Interestingly, U20 is also phosphorylated on at least one Ser, Thr, or Tyr residue. These results provide a framework to understand the role(s) of U20 in evading host defenses.

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“…The over-expression of A3G was measured by transfecting pcDNA3.1-APOBEC3G-HA into cells. Hop-8 upregulated A3G mRNA expression which was turned on by the CMV promotor as well as PMA [ 52 ]. However, the enhancing effect was not as significant as in PBMCs.…”

Section: Discussionmentioning

confidence: 99%

Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae

et al. 2017

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APOBEC3G is a member of the human cytidine deaminase family that restricts Vif-deficient viruses by being packaged with progeny virions and inducing the G to A mutation during the synthesis of HIV-1 viral DNA when the progeny virus infects new cells. HIV-1 Vif protein resists the activity of A3G by mediating A3G degradation. Phorbol esters are plant-derived organic compounds belonging to the tigliane family of diterpenes and could activate the PKC pathway. In this study, we identified an inhibitor 12-O-tricosanoylphorbol-20-acetate (hop-8), a novel ester of phorbol which was isolated from Ostodes katharinae of the family Euphorbiaceae, that inhibited the replication of wild-type HIV-1 and HIV-2 strains and drug-resistant strains broadly both in C8166 cells and PBMCs with low cytotoxicity and the EC50 values ranged from 0.106 μM to 7.987 μM. One of the main mechanisms of hop-8 is to stimulate A3G expressing in HIV-1 producing cells and upregulate the A3G level in progeny virions, which results in reducing the infectivity of the progeny virus. This novel mechanism of hop-8 inhibition of HIV replication might represents a promising approach for developing new therapeutics for HIV infection.

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Transgene expression enhancement in T-lymphoma cell lines

Cited by 10 publications

References 21 publications

Involvement of Thyroid Hormones in the Alterations of T-Cell Immunity and Tumor Progression Induced by Chronic Stress

Involvement of Thyroid Hormones in the Alterations of T-Cell Immunity and Tumor Progression Induced by Chronic Stress

Characterization of the HHV-6 U20 immunoevasin

Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae

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