Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope

Wang, Xinhai; Kochetkova, Irina; Haddad, Asmahan; Hoyt, Teri; Hone, David M.; Pascual, David W.

doi:10.1016/j.vaccine.2005.02.023

Cited by 34 publications

(22 citation statements)

References 44 publications

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“…When UEA-1 was used as an Ag delivery vehicle, the administration of PP M cell-targeted Ags induced Agspecific mucosal and systemic immune responses (16) despite the cospecificity of the lectin for M cells and goblet cells (3). The gene-profiling database was, therefore, used to look for candidate target molecules in the vaccine delivery system.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Terahara

Yoshida

Igarashi

et al. 2008

The Journal of Immunology

163

150

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4 of both GALT and nasopharynx-associated lymphoid tissue, which act as a major inductive site for Ag-specific mucosal immune responses (1, 2). Recently, we also identified M cells in the small intestinal villous epithelium, at effector sites far from the FAE, suggesting that Ag sampling via villous M cells may be responsible for induction of systemic Ag-specific immune responses, such as IgG production via the oral route (3). Still missing, however, were a characterization of the shared and distinctive traits of Peyer's patches (PPs) and villous M cells and a better understanding of the immunological nature of each.Recent comprehensive gene expression analyses using microdissected FAE or whole cells dissociated from the FAE identified genes specifically expressed by PP M cells (4 -6). Similar data, however, have not been available for villous M cells, in part because sufficient numbers of M cells are difficult to isolate from the surrounding intestinal epithelial cells (IECs). In mice, lectin Ulex europaeus agglutinin-1 (UEA-1) possessing affinity for ␣ (1, 2) fucose has been routinely used for the detection of such M cells (3, 7). UEA-1, however, does not alone suffice to identify M cells because it also reacts to goblet cells (3). Our laboratory has recently succeeded in distinguishing M cells from goblet cells by developing a mAb (NKM 16-2-4 mAb) that specifically reacts to murine PP and villous M cells but not goblet cells and IECs (8). Furthermore, our recent separate studies have demonstrated that oral administration of cholera toxin (CT) as mucosal adjuvant resulted in the induction of NKM 16-2-4 mAb ϩ and UEA-1 ϩ M-like cells, which have pocket structure and Ag uptake ability, in the duodenal villous epithelium (Terahara et al., submitted for publication). These recent advances in our understanding of M cells allowed us to define gene expression profiles capable of distinguishing PP M cells, CT-induced villous M-like cells, and IECs. Materials and Methods AnimalsBALB/c mice were purchased from Japan SLC. These mice were maintained under specific pathogen-free conditions in horizontal flow cabinets in our experimental animal facility at the University of Tokyo. Following a previously established protocol (9, 10), CT (List Biologic Laboratories) was dissolved in PBS (20 g/mouse) and then orally administered to BALB/c mice. Two days after CT administration, mice were used for experiments. All animal experiments were approved by the Animal Care and Use Committee of University of Tokyo. Lectins and Abs for the detection of M cellsThe following fluorescence-conjugated lectins and Abs were used for the identification of PP and villous M cells by FACS and histochemistry: PEconjugated UEA-1 (Biogenesis), rhodamine-conjugated UEA-1 (Vector

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Section: Discussionmentioning

confidence: 99%

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Terahara

Yoshida

Igarashi

et al. 2008

The Journal of Immunology

163

150

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“…Thus, markedly different overall patterns of lectin immunostaining were observed in vestigial vomeronasal organs of humans and chimpanzees compared with chemosensory vomeronasal organs in other primates (Kinzinger et al, 2005). Furthermore, knowledge of the lectin-binding patterns in the olfactory and nasal epithelia is a prerequisite for lectin-mediated DNA delivery during targeted mucosal immunization (Wang et al, 2005). …”

Section: Lectin Bindingmentioning

confidence: 99%

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Knabe¹,

Washausen²

2015

Primate Biol.

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Abstract. The longstanding debate on the taxonomic status of Tupaia belangeri (Tupaiidae, Scandentia, Mammalia) has persisted in times of molecular biology and genetics. But way beyond that Tupaia belangeri has turned out to be a valuable and widely accepted animal model for studies in neurobiology, stress research, and virology, among other topics. It is thus a privilege to have the opportunity to provide an overview on selected aspects of neural development and neuroanatomy in Tupaia belangeri on the occasion of this special issue dedicated to Hans-Jürg Kuhn. Firstly, emphasis will be given to the optic system. We report rather "unconventional" findings on the morphogenesis of photoreceptor cells, and on the presence of capillary-contacting neurons in the tree shrew retina. Thereafter, network formation among directionally selective retinal neurons and optic chiasm development are discussed. We then address the main and accessory olfactory systems, the terminal nerve, the pituitary gland, and the cerebellum of Tupaia belangeri. Finally, we demonstrate how innovative 3-D reconstruction techniques helped to decipher and interpret so-far-undescribed, strictly spatiotemporally regulated waves of apoptosis and proliferation which pass through the early developing forebrain and eyes, midbrain and hindbrain, and through the panplacodal primordium which gives rise to all ectodermal placodes. Based on examples, this paper additionally wants to show how findings gained from the reported projects have influenced current neuroembryological and, at least partly, medical research.

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“…Some studies showed that mucosal vaccine delivery can be improved using appropriate M‐cell targeting molecules such as lectins, its peptide mimetics and antibodies (Abs) specific to a fucose moiety presented at the surface of mouse (but not human) M cells (Wang et al. 2005; Nochi et al. 2007; Misumi et al.…”

Section: Introductionmentioning

confidence: 99%

Royal jelly enhances antigen‐specific mucosal IgA response

Kai

Motomura

Saito

et al. 2013

Food Science & Nutrition

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The effective uptake of antigens (Ags) by specialized M cells of gut‐associated lymphoid tissues is an important step in inducing an efficient intestinal mucosal immune response. In this study, royal jelly (RJ) was found to stimulate the differentiation of M‐like cells from human Caco‐2 cells in an in vitro M cell model. Furthermore, RJ and protease‐treated royal jelly (pRJ) efficiently enhanced transcytosis of FluoSpheres® carboxylate‐modified microspheres from the apical side to the basolateral side in the model. Therefore, we evaluated the ability of pRJ to induce efficient mucosal immune responses in an in vivo nonhuman primate. Continuous oral administration of commercially available pRJ resulted in a significant enhacement of the antigen‐specific IgA response in stool sample. Interestingly, Caco‐2 monolayer assay demonstrated that ether extracts from pRJ efficiently increased the expression level of a universal M cell marker, glycoprotein 2 (gp2). These findings suggest that pRJ exhibits mucosal immunomodulatory properties via stimulation of effective uptake of Ags through M cells.

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Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope

Cited by 34 publications

References 44 publications

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Royal jelly enhances antigen‐specific mucosal IgA response

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Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope

Cited by 34 publications

References 44 publications

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Comprehensive Gene Expression Profiling of Peyer’s Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells

Early development of the nervous system of the eutherian &lt;i&gt;Tupaia belangeri&lt;/i&gt;

Royal jelly enhances antigen‐specific mucosal IgA response

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Resources

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Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>