Transgenerational Evaluation of Elateriospermum tapos Extracts on the Male Offspring of Obesity-Induced Sprague Dawley Rats

Abstract

Obesity has been considered as a great public health concern, that has spread in both economic and poor resources countries. This study aimed to investigate the effect of Elateriospermum tapos supplementation on the male offspring of female obesity-induced Sprague Dawley (SD) rats at weaning and adult age. A total of thirty (30) female and fifteen (15) male Sprague Dawley rats (N=45) were purchased for this study. Of the 30 female rats, six (n=6) were randomly selected as the control group (CG) and fed separately with male on standard chow diet, while the remaining rats (n=24) were fed on a high-fat diet for 5 weeks. The obese group were further randomly divided into 4 groups, positive control group (PG), orlistat treatment (DG) at 200 mg/kg, treatment 1 (TX1, 200 mg/kg E. tapos seed) and treatment 2 (TX2, 200 mg/kg E. tapos shell) for 6 weeks. One male pup from each dam was culled at weaning (postnatal day 21 (PND21)) and adulthood (12 weeks). The liver, kidney, retroperitoneal white adipose tissue (RpWAT) and brown adipose tissue (BAT) were collected for histopathological study. Serum lipid profiles, liver enzyme activities and creatinine were measured. The bodyweight of male offspring from treatment 1 (MTX1) and 2 (MTX2) was significantly lower (P<0.05) compare to MNG group. The RpWAT weight in MTX1 and MTX2 for adult offspring also were significantly lower (P<0.05) compared to MPG. The histopathological examination of liver in MCG, MDG, MTX1, and MTX2 showed normal hepatocytes while the MPG group showed the presence of ballooning cell and hypertrophy of adipocytes was also observed in MPG group compared to another group’s rat. The E. tapos extracts from the shell have greater therapeutic potential on maternal obesity in short and long term treatment.

Elateriospermum tapos Yogurt Supplement in Maternal Obese Dams during Pregnancy Modulates the Body Composition of F1 Generation

“…In addition, mothers who supplemented with the raw extract of E. tapos during pregnancy showed a gradual reduction in body weight and calorie intake, while the activity of lipoprotein lipase was highly suppressed. This effect has been witnessed not only in the mothers but also in the offspring [11], which proves that E. tapos extract prevents the transgenerational inheritance of obesity and the obese gene product known as leptin in the offspring [12]. Studies claim that the natural anti-obesity effect of E. tapos could be due to the presence of various phytochemical compounds such as α-glucosidase, α-amylase, and pancreas lipase inhibitor enzymes, which inhibit fat and carbohydrate absorption.…”

Preliminary Study on Enhancing Students’ Sight Singing and Ear Training Abilities through the Integration of Kodaly Teaching Method and Chinese Folk Songs

“…Balan evaluated the effectiveness of using a 3D rat model for teaching blood withdrawal techniques among undergraduate students in medical education [9] . The utilization of 3D models in teaching is crucial in the context of 21st-century teaching methods.…”

Phytochemical component and toxicological evaluation of purple sweet potato leaf extract in male Sprague–Dawley rats

“…All organs (liver, kidney, pancreas, retina, aorta, and heart) fixed in 10% of NBF were sectioned to obtain paraffin ribbons, followed by fishing of the ribbon from the water bath (40°C–45°C) and fixation to a glass slide. The slides were then stained with H&E as described elsewhere and observed by microscopy ( Balan et al, 2021 ). All images were captured and verified by a certified pathologist from UPM.…”