Transgenic Cotton: From Biotransformation Methods to Agricultural Application

Zhang, Baohong

doi:10.1007/978-1-4939-8952-2_1

Cited by 10 publications

(8 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are three major methods for cotton transformation: the pollen tube pathway-mediated method, the biolistic particle delivery system and Agrobacterium -mediated genetic transformation. Among them, Agrobacterium -mediated genetic transformation is the most widely used in cotton genetic transformation 16 . In our study, we delivered the CRISPR/Cas9 system into the cotton genome using the Agrobacterium -mediated method with the shoot apexes as receptors ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

Chen

Shu

et al. 2017

Sci Rep

107

View full text Add to dashboard Cite

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system has been widely used for genome editing in various plants because of its simplicity, high efficiency and design flexibility. However, to our knowledge, there is no report on the application of CRISPR/Cas9-mediated targeted mutagenesis in cotton. Here, we report the genome editing and targeted mutagenesis in upland cotton (Gossypium hirsutum L., hereafter cotton) using the CRISPR/Cas9 system. We designed two guide RNAs to target distinct sites of the cotton Cloroplastos alterados 1 (GhCLA1) and vacuolar H+-pyrophosphatase (GhVP) genes. Mutations in these two genes were detected in cotton protoplasts. Most of the mutations were nucleotide substitutions, with one nucleotide insertion and one substitution found in GhCLA1 and one deletion found in GhVP in cotton protoplasts. Subsequently, the two vectors were transformed into cotton shoot apexes through Agrobacterium-mediated transformation, resulting in efficient target gene editing. Most of the mutations were nucleotide deletions, and the mutation efficiencies were 47.6–81.8% in transgenic cotton plants. Evaluation using restriction-enzyme-PCR assay and sequence analysis detected no off-target mutations. Our results indicated that the CRISPR/Cas9 system was an efficient and specific tool for targeted mutagenesis of the cotton genome.

show abstract

Section: Discussionmentioning

confidence: 99%

Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

Chen

Shu

et al. 2017

Sci Rep

107

View full text Add to dashboard Cite

show abstract

“…Cotton is the most important fiber crop with significant economic value. For the improvements in cotton productivity and fiber quality, transgenic cottons have been developed as the first commercial transgenic crops, by leveraging Agrobacterium -mediated genetic transformation and plant regeneration via somatic embryogenesis ( Zhang, 2019 ). Despite the overt success in commercial exploitation, broad application of transgenic technology in cotton is constrained by the genotypic variation in the capability of plant regeneration from tissue culture as the vast majority of elite cotton germplasms are recalcitrant to the present transgenic protocols ( Leelavathi et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Characterization of the JmjC Domain-Containing Histone Demethylase Gene Family Reveals GhJMJ24 and GhJMJ49 Involving in Somatic Embryogenesis Process in Cotton

Zhu

Yao

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

The Jumonji C (JmjC) domain-containing protein family, an important family of histone demethylase in plants, can directly reverse histone methylation and play important roles in various growth and development processes. In the present study, 51 JmjC genes (GhJMJs) were identified by genome-wide analysis in upland cotton (Gossypium hirsutum), which can be categorized into six distinct groups by phylogenetic analysis. Extensive syntenic relationship events were found between G. hirsutum and Theobroma cacao. We have further explored the putative molecular regulatory mechanisms of the JmjC gene family in cotton. GhJMJ24 and GhJMJ49 were both preferentially expressed in embryogenic callus compared to nonembryogenic callus in cotton tissue culture, which might be regulated by transcription factors and microRNAs to some extent. Further experiments indicated that GhJMJ24 and GhJMJ49 might interact with SUVH4, SUVH6, DDM1, CMT3, and CMT1 in the nucleus, potentially in association with demethylation of H3K9me2. Taken together, our results provide a foundation for future research on the biological functions of GhJMJ genes in cotton, especially in somatic embryogenesis in cotton tissue culture, which is crucial for the regeneration of transgenic plants.

show abstract

“…Cotton is an important cash crop worldwide, and provides natural fiber as material for the textile industry, oil as a food additive, and protein as feedstuff ( Ruan, 2013 ; Fang et al, 2017 ; Zhang, 2019 ). However, cotton plants experience various biotic stresses including pests and pathogens, which lead to great loss of yield and reduced fiber quality ( Sattar et al, 2013 ; Phillips et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Cotton miR393-TIR1 Module Regulates Plant Defense Against Verticillium dahliae via Auxin Perception and Signaling

Shi

Wang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Plant auxin is essential in plant growth and development. However, the molecular mechanisms of auxin involvement in plant immunity are unclear. Here, we addressed the function of the cotton (Gossypium hirsutum) miR393-TIR1 module in plant defense against Verticillium dahliae infection via auxin perception and signaling. GhTIR1 was directedly cleaved by ghr-miR393 according to mRNA degradome data, 5′-RACE analysis, and a GUS reporter assay. Ghr-miR393 knockdown significantly increased plant susceptibility to V. dahliae compared to the control, while ghr-miR393 overexpression and GhTIR1 knockdown significantly increased plant resistance. External indole-3-acetic acid (IAA) application significantly enhanced susceptibility to V. dahliae in ghr-miR393 knockdown and control plants compared to mock treatment, and only slightly increased susceptibility in overexpressing ghr-miR393 and GhTIR1-silenced plants. Application of external PEO-IAA (an auxin antagonist) had a contrary trend with IAA application. Based on yeast two-hybrid and bimolecular fluorescence complementation assays, GhTIR1 interacted with GhIAA14 in the nucleus, and GhIAA14 knockdown reduced plant resistance to V. dahliae infection. The results suggested that the ghr-miR393-GhTIR1 module regulates plant defense via auxin perception and signaling. Additionally, simultaneous knockdown of GhTIR1 and GhICS1 significantly increased plant susceptibility to V. dahliae compared to the control, indicating that salicylic acid (SA) accumulation is vital for the ghr-miR393-GhTIR1 module to regulates plant resistance. Transcriptome data also demonstrated that GhTIR1 knockdown significantly downregulated expression of auxin-related genes and upregulated expression of SA-related genes. Overall, the ghr-miR393-GhTIR1 module participates in plant response to V. dahliae infection via IAA perception and signaling partially depending on the SA defense pathway.

show abstract

Transgenic Cotton: From Biotransformation Methods to Agricultural Application

Cited by 10 publications

References 100 publications

Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system

Genome-wide Characterization of the JmjC Domain-Containing Histone Demethylase Gene Family Reveals GhJMJ24 and GhJMJ49 Involving in Somatic Embryogenesis Process in Cotton

Cotton miR393-TIR1 Module Regulates Plant Defense Against Verticillium dahliae via Auxin Perception and Signaling

Contact Info

Product

Resources

About