TRANSGENIC COTTON:HARBORING BROAD TERM RESISTANCE AGAINST INSECT AND WEEDS THROUGH INCORPORATION OF CEMB DOUBLE Bt AND cp4EPSPS GENES

Awan, Mudassar Fareed; Ali, Arfan; Muzaffar, Adnan; Abbas, Mohammed Abdulridha; Rao, Abdul Qayyum; Qamar, Zahida; Butt, Shahid Javed; Khan, Ghazanfar Ali; Rashid, Bushra; Nasir, Idrees Ahmad; Husn, Tayyab

doi:10.21162/pakjas/16.2007

Cited by 3 publications

(8 citation statements)

References 15 publications

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“…Comparison with other studies revealed that G. arboreum has more expression than G. hirsutum . 11 , 21 In this study, Cry1Ac protein concentration is well above (3.534µg g −1 ) the LD95 concentration of 2.20 µg g −1 as determined by. 33 and 159 µg g −1 as determined by.…”

Section: Discussionsupporting

confidence: 61%

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Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton)

et al. 2021

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Gossypium arboreum (Desi Cotton) holds a special place in cotton industry because of its inherent ability to withstand drought, salinity, and remarkable resistance to sucking pests and cotton leaf curl virus. However, it suffers yield losses due to weeds and bollworm infestation. Genetic modification of G. arboreum variety FBD-1 was attempted in the current study to combat insect and weedicide resistance by incorporating cry1Ac, cry2A and cp4-EPSPS genes under control of 35S promoter in two different cassettes using kanamycin and GUS as markers through Agrobacterium -mediated shoot apex cut method of cotton transformation. The efficiency of transformation was found to be 1.57%. Amplification of 1700 bp for cry1Ac , 167 bp for cry2A and 111 bp for cp4-EPSPS confirmed the presence of transgenes in cotton plants. The maximum mRNA expression of cry1Ac and cp4-EPSPS was observed in transgenic cotton line L3 while minimum in transgenic cotton line L1. The maximum protein concentrations of Cry1Ac, Cry2A and Cp4-EPSPS of 3.534 µg g −1 , 2.534 µg g −1 and 3.58 µg-g −1 respectively were observed for transgenic cotton line L3 as compared to control cotton line. On leaf-feed-based insect bioassay, almost 99% mortality was observed for Helicoverpa armigera on the transgenic cotton plant (L3). It completely survived the 1900 ml hectare −1 glyphosate spray assay as compared to non-transgenic cotton plants. The necrotic spots appeared on the third day, leading to the complete death of control plants on the fifth day of assay. The successful multiple gene-stacking in G. arboreum FBD-1 variety could be further used for qualitative improvement of cotton fiber through plant breeding techniques.

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Section: Discussionsupporting

confidence: 61%

“…(b) depicts the obtained concentration of Cp4-EPSPS, Cry1Ac and Cry2a in tetraploid cotton ( Gossypium hirsutum ) as determined in different studies. 11 , 14 , 21–23 …”

Section: Resultsmentioning

confidence: 99%

Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton)

et al. 2021

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“…Temperature plays a major role in deciding the sowing time and accordingly the length of critical phenophases to manipulate the crop production (Tewari and Singh, 1993;Hussain et al, 2012a). Correct timing of different phenological events and their link with yield related traits is pre-requisite to augment crop productivity under given set of climatic conditions (Gbmez-Macpherson and Richards, 1997;Hussain et al, 2012a,b;Awan et al, 2016). With the inclusion of BT cotton in the cotton-wheat cropping system, most of the farmers sow cotton early to achieve its maximum production potential, however, research on optimization of the best sowing tome of BT cotton is lacking.…”

Section: Introductionmentioning

confidence: 99%

Yield and Phenological Responses of Bt Cotton to Different Sowing Dates in Semi-Arid Climate

Shah

Farooq

Shahzad

et al. 2017

PAKJAS

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With the inclusion of BT cotton in the existing cotton-wheat cropping system of South Punjab, Pakistan; farmers are interested to sow early BT cotton to obtain its full production potential. There is a problem of sub and supra optimum temperature due to early and late sowing of BT cotton. Little efforts have been done to optimize the best sowing date of BT cotton in cotton-wheat cropping system. In this study, popular BT cotton cultivar MNH-886 was sown from February 14 till May 15, with two weeks interval, in soil filled earthen pots kept under natural conditions. Early sown (planted on February 14 and March 01) crop took more days to start emergence, complete 50% emergence and mean emergence time, and had minimum plant emergence. However, late sown (30 th April and 15 th May) took less time to germinate, with better and uniform seedling establishment. Although early planted crop took more days to start emergence, squaring, flowering, boll formation and boll opening but growing degree days to start emergence, squaring, flowering, boll formation and boll opening were high in late sown crop. However, BT cotton sown on 16 th March yielded better than all other sowing dates owing to increase in yield related traits like number of sympodial branches, bolls per plant and boll weight; while crop sown on 15 th May didn't perform well. Likewise sowing of BT cotton on 16 th March had better fiber quality in terms of ginning out turn, fiber length, strength, fineness and uniformity. There was a positive correlation of phenological events like days to start squaring, flowering, boll formation and boll opening with seed cotton and lint yield of BT cotton; and cotton yield and boll weight were also positively linked with fiber quality traits. In conclusion, planting of BT cotton on 16 th March is the best option to harvest higher seed cotton and lint yield of better quality fiber under arid to semi-arid conditions of South Punjab, Pakistan.

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“…However, all these crops were resistant against specific kind of herbicide, while transgenics with c p 4EPSPS are not confined with single kind of herbicid 27 , 66 . Up till now, nine glyphosate-resistant crops have been introduced, comprising soybean, wheat, canola, polish canola, corn, alfalfa, sugar beet, creeping bent-grass and cotton 17 , 37 .…”

Section: Discussionmentioning

confidence: 99%

“…Globally, pests along with diseases cause 37% loss to the agriculture production from which 13% is just because of insects 16 , 17 . Approximately 1300, insect pests attack on sugarcane crop around the world.…”

Section: Introductionmentioning

confidence: 99%

Novel approaches to circumvent the devastating effects of pests on sugarcane

Qamar

Nasir

AbouHaidar

et al. 2021

Sci Rep

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Sugarcane (Saccharum officinarum L.) is a cash crop grown commercially for its higher amounts of sucrose, stored within the mature internodes of the stem. Numerous studies have been done for the resistance development against biotic and abiotic stresses to save the sucrose yields. Quality and yield of sugarcane production is always threatened by the damages of cane borers and weeds. In current study two problems were better addressed through the genetic modification of sugarcane for provision of resistance against insects and weedicide via the expression of two modified cane borer resistant CEMB-Cry1Ac (1.8 kb), CEMB-Cry2A (1.9 kb) and one glyphosate tolerant CEMB-GTGene (1.4 kb) genes, driven by maize Ubiquitin Promoter and nos terminator. Insect Bio-toxicity assays were carried out for the assessment of Cry proteins through mortality percent of shoot borer Chilo infuscatellus at 2nd instar larvae stage. During V0, V1 and V2 generations young leaves from the transgenic sugarcane plants were collected at plant age of 20, 40, 60, 80 days and fed to the Chilo infuscatellus larvae. Up to 100% mortality of Chilo infuscatellus from 80 days old transgenic plants of V2 generation indicated that these transgenic plants were highly resistant against shoot borer and the gene expression level is sufficient to provide complete resistance against target pests. Glyphosate spray assay was carried out for complete removal of weeds. In V1-generation, 70–76% transgenic sugarcane plants were found tolerant against glyphosate spray (3000 mL/ha) under field conditions. While in V2-generation, the replicates of five selected lines 4L/2, 5L/5, 6L/5, L8/4, and L9/6 were found 100% tolerant against 3000 mL/ha glyphosate spray. It is evident from current study that CEMB-GTGene, CEMB-Cry1Ac and CEMB-Cry2A genes expression in sugarcane variety CPF-246 showed an efficient resistance against cane borers (Chilo infuscatellus) and was also highly tolerant against glyphosate spray. The selected transgenic sugarcane lines showed sustainable resistance against cane borer and glyphosate spray can be further exploited at farmer’s field level after fulfilling the biosafety requirements to boost the sugarcane production in the country.

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TRANSGENIC COTTON:HARBORING BROAD TERM RESISTANCE AGAINST INSECT AND WEEDS THROUGH INCORPORATION OF CEMB DOUBLE Bt AND cp4EPSPS GENES

Cited by 3 publications

References 15 publications

Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton)

Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton)

Yield and Phenological Responses of Bt Cotton to Different Sowing Dates in Semi-Arid Climate

Novel approaches to circumvent the devastating effects of pests on sugarcane

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