Transgenic Plant Cell Cultures: A Promising Approach for Secondary Metabolite Production

Satish, Lakkakula; Rency, Arockiam Sagina; Muthubharathi, Balasubramanian Chellammal; Shamili, Sasanala; Ramesh, Manikandan; Swamy, Mallappa Kumara

doi:10.1007/978-981-13-7438-8_4

Cited by 12 publications

(5 citation statements)

References 329 publications

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“…These results shown that radical scavenging activity of A. longiloba extracts could be mostly related to their TPC. The anti-free-radicals property of phenolic compounds depends on their molecular structures, particularly the availability of phenolic hydrogens leads to the formation of phenoxyl radicals by hydrogen donation and confers to phenolic compounds the ability of in-activating free radicals [ 54 , 55 , 56 ]. Using the DPPH assay, the antioxidant activities of A. longiloba field-grown plant parts and in vitro-derived callus extract were ranked in the order in vitro-derived callus > petiole > fruit.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Abdulhafiz

Mohammed

Kayat

et al. 2020

Plants

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In this study, an efficient micropropagation protocol was developed for A. longiloba and the antioxidant properties of field-grown plant, in vitro-derived greenhouse-grown plant and in vitro-derived callus extracts were compared. The A. longiloba seeds tested using tetrazolium chloride salt exhibited 89% viability. Due to poor germination capacity of A. longiloba seeds, the seeds were treated with gibberellic acid (GA3) or sulfuric acid (H2SO4). The maximum seed germination of 87% was observed at 30% H2SO4 treatment after 19.00 d, whereas GA3 treatment showed maximum germination of 53% after 22 d. In vitro shoot multiplication was carried out using various types of cytokinins alone or in combination with auxin. Among them, 6-benzyl amino purine (BAP) single treatment was found to be the best hormone. The highest shoot-length (7.26 cm) and maximum number of shoots per explant (18) were recorded at 3-mg L−1 BAP. For in vitro rooting, indole-3-acetic acid at 0.5-mg L−1 was found to be the optimum concentration. Callus was induced using various types of auxins alone or in combinations with cytokinins. The highest percentage of callus of 91 and fresh weight of 6 g was obtained with 3-mg L−1 IAA. The plantlets produced in the current study were subjected to acclimatization. The combination of topsoil and peat moss at 1:2 ratio was found to be the best soil media. In this study, in vitro-derived callus extract showed the highest phenolic content (538 mg GAE), followed by extracts of field-grown plant parts, i.e., fruit and petiole (504 and 300 mg GAE) while in vitro plant extract showed the lowest (98 mg GAE). Meanwhile, the highest flavonoids was recorded in petiole extract. Comparative antioxidant activity study shows, in vitro-derived callus exhibited better DPPH-radical-scavenging activity (IC50: 0.113-mg mL−1) whereas the extracts of petiole, fruit and in vitro plant showed 0.126-, 0.137- and 0.173-mg mL−1, respectively. At the same time, the fruit extract showed better (IC50: 0.088-mg mL−1) ABTS radical scavenging activity than all extracts tested. In conclusion, the in vitro-derived callus extract could be favored for high TPC and better DPPH scavenging activity. Hence, the present study was conducted to establish an efficient micropropagation protocol and to compare the antioxidant activity of the field-grown plant, in vitro plant and in vitro derived callus extracts of A. longiloba.

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Section: Resultsmentioning

confidence: 99%

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Abdulhafiz

Mohammed

Kayat

et al. 2020

Plants

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“…( Ochoa‐Villarreal et al., 2016 ; Georgiev et al., 2018 ). Because of its ability to minimize wrinkles in the crow’s feet area of the face, a liposome-encapsulated extract of cultured apple stem cells is employed as an ideal ingredient in anti-aging products ( Satish et al., 2019 ; Krasteva et al., 2020 ; Krol et al., 2020 ). Chemicals isolated from Catharanthusroseus are currently used in the manufacturing of both ordinary consumer and professional care cosmetics, demonstrating significant potential in protecting skin from heavy metal toxicity ( Mallurwar and Pathak, 2008 ; Schmid and Zülli, 2012 ; Blum et al., 2013 ; Hayta et al., 2017 ; Meir et al., 2018 ).…”

Section: Applications Of Plant Tissue Culturementioning

confidence: 99%

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

et al. 2022

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Plant tissue culture technique employed for the identification and isolation of bioactive phytocompounds has numerous industrial applications. It provides potential benefits for different industries which include food, pharmaceutical and cosmetics. Various agronomic crops i.e., cereals, fruits, vegetables, ornamental plants and forest trees are currently being used for in vitro propagation. Plant tissue culture coupled with biotechnological approaches leads towards sustainable agricultural development providing solutions to major food security issues. Plants are the rich source of phytochemicals with medicinal properties rendering them useful for the industrial production of pharmaceuticals and nutraceuticals. Furthermore, there are numerous plant compounds with application in the cosmetics industry. In addition to having moisturizing, anti‐ageing, anti‐wrinkle effects; plant-derived compounds also possess pharmacological properties such as antiviral, antimicrobial, antifungal, anticancer, antioxidant, anti-inflammatory, and anti-allergy characteristics. The in vitro propagation of industrially significant flora is gaining attention because of its several advantages over conventional plant propagation methods. One of the major advantages of this technique is the quick availability of food throughout the year, irrespective of the growing season, thus opening new opportunities to the producers and farmers. The sterile or endangered flora can also be conserved by plant micro propagation methods. Hence, plant tissue culture is an extremely efficient and cost-effective technique for biosynthetic studies and bio-production, biotransformation, or bioconversion of plant-derived compounds. However, there are certain limitations of in-vitro plant regeneration system including difficulties with continuous operation, product removal, and aseptic conditions. For sustainable industrial applications of in-vitro regenerated plants on a large scale, these constraints need to be addressed in future studies.

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“…Even though the titer of biologically active secondary metabolites is normally low in plant cells, they can be engineered to increase yields or target compounds [2, 5]. Moreover, plants readily undergo genetic transformation, which could grant them the ability to produce heterologous proteins of pharmaceutical importance [8, 9]. As a result, the strategy for “molecular farming” has been employed for mass production of various pharmaceuticals by using genetically modified plants and plant in vitro systems [9–11].…”

Section: Cultivation Of Plant Cell Tissue and Organ Cultures With Emmentioning

confidence: 99%

“…Moreover, plants readily undergo genetic transformation, which could grant them the ability to produce heterologous proteins of pharmaceutical importance [8, 9]. As a result, the strategy for “molecular farming” has been employed for mass production of various pharmaceuticals by using genetically modified plants and plant in vitro systems [9–11]. For example, the first plant‐made antibodies tested in a clinical trial were commercialized by Planet Biotechnology Inc (https://www.planetbiotechnology.com/index.html) [11].…”

Section: Cultivation Of Plant Cell Tissue and Organ Cultures With Emmentioning

confidence: 99%

Recent applications of plant cell culture technology in cosmetics and foods

Krasteva

Georgiev

Pavlov

2020

Engineering in Life Sciences

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Plants have been used as the main source of phytochemicals with nutritional, medicinal, cultural and cosmetic applications since times immemorial. Nowadays, achieving sustainable development, global climate change, restricted access to fresh water, limited food supply and growing energy demands are among the critical global challenges faced by humanity. Plant cell culture technology has the potential to address some of these challenges by providing effective tools for sustainable supply of phyto‐ingredients with reduced energy, carbon and water footprints. The main aim of this review is to discuss the recent trends in the development of plant cell culture technologies for production of plant‐derived substances with application in food products and cosmetic formulations. The specific technological steps and requirements for the final products are discussed in the light of the advances in cultivation technologies used for growing differentiated and undifferentiated plant in vitro systems. Future prospects and existing challenges of the commercialization of plant cell culture‐derived products have been outlined through the prism of the authors’ point of view. We expect this review will encourage scientists, policymakers and business enterprises to join efforts for speeding‐up the mass commercialization and popularization of plant cell culture technology as an eco‐friendly alternative method for sustainable production of plant‐derived additives with application in food and cosmetic products.

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Transgenic Plant Cell Cultures: A Promising Approach for Secondary Metabolite Production

Cited by 12 publications

References 329 publications

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Micropropagation of Alocasia longiloba Miq and Comparative Antioxidant Properties of Ethanolic Extracts of the Field-Grown Plant, In Vitro Propagated and In Vitro-Derived Callus

Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

Recent applications of plant cell culture technology in cosmetics and foods

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