Transgenic Potato (Solanum tuberosum L.) Plants Expressing the Gene of Secretory Nuclease from Serratia marcescens

Trifonova, E. A.; Komarova, M. L.; Leonova, N. S.; Shcherban, A. B.; Кочетов, А. В.; Malinovskii, V. I.; Shumny, V. K.

doi:10.1023/b:dobi.0000017151.98825.d5

Cited by 4 publications

(6 citation statements)

References 8 publications

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“…Potato varieties differ among themselves for a large number of characteristics, starting from the shape of the tuber and taste qualities and ending with resistance to phytopathogens. In our previous research of the expression of heterologous nucleases in transgenic plants, we noticed that different potato varieties have different intrinsic levels of RNase activity (Trifonova et al, 2004). In this study, we first assessed this level for 16 varieties listed in the Federal State Register of Breeding Achievements Admitted to Use in the Russian Federation (2017) and checked whether the level of RNase activity is related to some important agricultural characteristics such as maturity, potato resistance to viral diseases, late blight and common scab.…”

Section: Resultsmentioning

confidence: 99%

Ribonuclease activity as a new prospective disease resistance marker in potato

et al. 2019

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Disease resistance is an important characteristic for each variety of potato, and the search for pathogen resistance markers is one of the primary tasks of plant breeding. Higher plants possess a wide spectrum of enzymes catalyzing the hydrolysis of nucleic acids; it is believed that protection against pathogens is the most probable function of the enzymes. RNases are actively involved in several immune systems of higher plants, for example, systemic acquired resistance (SAR) and genetic silencing, hence RNase activity in plant leaves, as a relatively easily measured parameter, can serve as a good marker for the selection of pathogen resistant varieties. We have analyzed sixteen varieties of potatoes permitted for use on the territory of the Russian Federation and tested the correlation of the level of varietyspecifc ribonuclease (RNase) activity with such economically valuable traits as maturity and resistance to viruses, late blight and common scab. In general, the level of RNase activity was varietyspecifc, which was confrmed by very small values of average squared error for the majority of tested varieties. We have detected a statistically signifcant positive correlation of RNase activity in potato leaves with increased resistance of varieties to phytopathogenic viruses, a negative correlation with resistance to scab and an absence of a signifcant connection with maturity and resistance to late blight, regardless of the organ affected by the oomycete. Thus, the level of RNase activity in potato leaves can be used as a selective marker for resistance to viruses, while varieties with increased RNase activity should be avoided when selecting resistance to scab.

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Section: Resultsmentioning

confidence: 99%

Ribonuclease activity as a new prospective disease resistance marker in potato

et al. 2019

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“…In addition to developing increased resistance against viral infection, bacterial barnases can participate in plant protection against other diseases. For example, transgenic tobacco plants producing barnase were shown to be protected from the late blight disease [71]. It has been found that potato plants expressing S. marcescens nuclease display enhanced resistance to pathogens [71,72].…”

Section: Bacterial Nucleases and Their Antiviral Activitymentioning

confidence: 99%

“…For example, transgenic tobacco plants producing barnase were shown to be protected from the late blight disease [71]. It has been found that potato plants expressing S. marcescens nuclease display enhanced resistance to pathogens [71,72]. Thus, genetic transformation using a bacterial RNase gene may be a promising approach for the engineering of plants with resistance to viral infection [73,74].…”

Section: Bacterial Nucleases and Their Antiviral Activitymentioning

confidence: 99%

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

Максимов

Sorokan

Бурханова

et al. 2019

Plants

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Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient and eco-friendly measures to prevent viral diseases. Since the genomic material of 90% higher plant viruses consists of single-stranded RNA, the best way to target the viral genome is to use ribonucleases (RNase), which can be effective against any viral disease of plants. Here, we show the importance of the search for endophytes with protease and RNase activity combined with the capacity to prime antiviral plant defense responses for their protection against viruses. This review discusses the possible mechanisms used to suppress a viral attack as well as the use of local endophytic bacteria for antiviral control in crops.

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“…For example, application of endonuclease of Serratia marsescens intensifies regeneration of potato plants from the apical explants and inhibits proliferation of DNA-and RNA-containing phytoviruses [5]. Antiviral action of endogenous plant ribonucleases, localized on the surface of plant cells in the apoplastis reported [16].…”

Section: The Effect Of Rnase Bp Treatment On Pvm and Pvs Elimination mentioning

confidence: 99%

“…They are less toxic and are readily decomposed without accumulation of harmful substances. It is known that some enzymes that are capable to break down the nucleic acids have antiviral activity high resistance to pathogens which have been observed in transgenic plant expressed bacterial nuclease Serratia marcescens [5]. Bacillus cereus ZH14 is found to produce a new type of antiviral ribonuclease, which is secreted into medium and active against tobacco mosaic virus [6] [7].…”

Section: Introductionmentioning

confidence: 99%

Antiviral Effect of Ribonuclease from <i>Bacillus pumilus</i> against Phytopathogenic Rna-Viruses

Шарипова¹,

Rockstroh²,

Balaban³

et al. 2015

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Transgenic Potato (Solanum tuberosum L.) Plants Expressing the Gene of Secretory Nuclease from Serratia marcescens

Cited by 4 publications

References 8 publications

Ribonuclease activity as a new prospective disease resistance marker in potato

Ribonuclease activity as a new prospective disease resistance marker in potato

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

Antiviral Effect of Ribonuclease from <i>Bacillus pumilus</i> against Phytopathogenic Rna-Viruses

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Transgenic Potato (Solanum tuberosum L.) Plants Expressing the Gene of Secretory Nuclease from Serratia marcescens

Cited by 4 publications

References 8 publications

Ribonuclease activity as a new prospective disease resistance marker in potato

Ribonuclease activity as a new prospective disease resistance marker in potato

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

Antiviral Effect of Ribonuclease from &lt;i&gt;Bacillus pumilus&lt;/i&gt; against Phytopathogenic Rna-Viruses

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Antiviral Effect of Ribonuclease from <i>Bacillus pumilus</i> against Phytopathogenic Rna-Viruses