Transgenic sugar beet tolerant to imidazolinone obtained by Agrobacterium-mediated transformation

Kishchenko, Olena; Komarnitskiĭ, I. K.; Кучук, М. В.

doi:10.3103/s0095452711030030

Cited by 5 publications

(2 citation statements)

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“…There is a considerable interest in generating herbicidetolerant cowpea plants. Imidazolinone tolerance was achieved by both conventional and molecular breeding in rice, soybean, sugar beet and sugarcane (Aragão et al Tan et al 2005;Stuart et al 2010;Vianna et al 2011, Kishchenko et al 2011. Line 59 showed high tolerance to up to 400 g ha -1 imazapyr, which is fourfold the commercial recommended dose for weed control in a number of crops.…”

Section: Discussionmentioning

confidence: 99%

“…Imidazolinone tolerance was achieved by both conventional and molecular breeding in rice (Tan et al 2005), Communicated by L. Peña. C. T. Citadin Á A. R. R. Cruz Á F. J. L. Aragão (&) Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, Brasília, DF 70.770-917, Brazil e-mail: francisco.aragao@embrapa.br maize (Newhouse et al 1991), oilseed rape (Swanson et al 1989), soybean (Aragão et al 2000), sugarcane (Stuart et al 2010), sugar beet (Kishchenko et al 2011) and cotton (Rajasekaran et al 1996). The mechanism of action of imidazolinone herbicides, such as imazapyr, is the inhibition of the enzymatic activity of acetohydroxyacid synthase (AHAS; acetolactate synthase, acetolactate pyruvate-lyase (carboxylating), EC 4.1.3.18), which catalyzes the initial step in the biosynthesis of isoleucine, leucine and valine (Shaner et al 1984).…”

Section: Introductionmentioning

confidence: 99%

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Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)

2013

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Here we present the development of cowpea lines tolerant to a herbicide from imidazoline class (imazapyr). Plants presented tolerance to fourfold the commercial recommended dose for weed control. Cowpea is one of the most important and widely cultivated legumes in many parts of the world. Its cultivation is drastically affected by weeds, causing damages during growth and development of plants, competing for light, nutrients and water. Consequently, weed control is critical, especially using no-tillage farming systems. In tropical regions, no-till farming is much easier with the use of herbicides to control weeds. This study was conducted to evaluate the possibility of obtaining transgenic cowpea plants resistant to imidazolinone, which would facilitate weed control during the summer season. The biolistic process was used to insert a mutated acetohydroxyacid synthase coding gene (Atahas) which confers tolerance to imazapyr. The transgene integration was confirmed by Southern blot analysis. Out of ten lines tested for tolerance to 100 g ha(-1) imazapyr, eight presented some tolerance. One line (named 59) revealed high herbicide tolerance and developmental growth comparable to non-transgenic plants. This line was further tested for tolerance to higher herbicide concentrations and presented tolerance to 400 g ha(-1) imazapyr (fourfold the commercial recommended dose) with no visible symptoms. Line 59 will be the foundation for generating imidazolinone-tolerant cowpea varieties, which will facilitate cultivation of this crop in large areas.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)

2013

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Shaping the Sugar Beet of Tomorrow: Current Advances in Sugar Beet Biotechnology and New Breeding Techniques

Mulet¹

2022

Sugar Beet Cultivation, Management and Processing

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Agronomic evaluation and molecular characterisation of the acetolactate synthase gene in imazapyr tolerant sugarcane (Saccharum hybrid) genotypes

et al. 2018

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Mutagenesis had no effect on number of stalks/plot, stalk height, fibre and sucrose content of mutants. Imazapyr tolerance is likely due to a S622N mutation in the acetolactate synthase gene. The herbicidal compound imazapyr is effective against weeds such as Cynodon and Rottboellia species that constrain sugarcane production. This study aimed to compare agronomic characteristics of three imazapyr tolerant mutants (Mut 1, Mut 6 and Mut 7) with the non-mutated N12 control after 18 months of growth, and to sequence the acetolactate synthase (ALS) gene to identify any point mutations conferring imazapyr tolerance. There were no significant differences in the number of stalks/plot, stalk height, fibre and sucrose contents of the mutants compared with the N12 control. However, Mut 1 genotype was more susceptible to the Lepidopteran stalk borer, Eldana saccharina when compared with the non-mutated N12 (11.14 ± 1.37 and 3.89 ± 0.52% internodes bored, respectively), making Mut 1 less desirable for commercial cultivation. Molecular characterisation of the ALS gene revealed non-synonymous mutations in Mut 6. An A to G change at nucleotide position 1857 resulted in a N513D mutation, while a G to A change at nucleotide position 2184 imposed a S622N mutation. Molecular dynamics simulations revealed that the S622N mutation renders an asparagine side chain clash with imazapyr, hence this mutation is effective in conferring imazapyr tolerance.

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Transgenic sugar beet tolerant to imidazolinone obtained by Agrobacterium-mediated transformation

Cited by 5 publications

References 45 publications

Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)

Development of transgenic imazapyr-tolerant cowpea (Vigna unguiculata)

Shaping the Sugar Beet of Tomorrow: Current Advances in Sugar Beet Biotechnology and New Breeding Techniques

Agronomic evaluation and molecular characterisation of the acetolactate synthase gene in imazapyr tolerant sugarcane (Saccharum hybrid) genotypes

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