Transglutaminase-mediated cross-linking of a peptic fraction of ω-5 gliadin enhances IgE reactivity in wheat-dependent, exercise-induced anaphylaxis

Palosuo, Kati; Varjonen, Elina; Nurkkala, Jenni; Kalkkinen, Nisse; Harvima, Rauno J.; Reunala, Timo; Alenius, Harri

doi:10.1067/mai.2003.1498

Cited by 143 publications

(92 citation statements)

References 42 publications

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“…that the cytokine interleukin (IL)-6, whose expression is 50-to 100-fold increased in marathon runners, upregulates tTG which by modifying especially x-5-gliadin proteins allows the formation of large peptide aggregates more effectively cross-linking the high-affinity IgE receptor FceRI (36). The latter hypothesis is based on in vitro experiments showing that beta-endorphin induces human mast cell degranulation and histamine release (37).…”

Section: Figurementioning

confidence: 99%

About the role and underlying mechanisms of cofactors in anaphylaxis

Wölbing

Fischer

Köberle

et al. 2013

Allergy

178

163

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Anaphylaxis is the systemic and most severe presentation of type I allergy. A number of conditions were identified that modulate the onset of anaphylaxis such as co-or augmentation factors, which significantly lower the allergen dose necessary for triggering anaphylaxis. Next to physical exercise or alcohol consumption, co-administration of nonsteroidal anti-inflammatory drugs (NSAID) or concomitant infectious diseases are well-documented cofactors of anaphylaxis. Registries for anaphylaxis document a role for cofactors in about 30% of anaphylactic reactions. Some disease entities such as 'wheat-dependent exercise-induced anaphylaxis' (WDEIA) are explicitly characterized by elicitation of anaphylaxis only in the presence of at least one such cofactor. Using WDEIA as a model disease, studies demonstrated that exercise increases skin prick test reactivity to and bioavailability of the allergen. Additional data indicate that alcohol consumption and NSAID administration display similar effects. Modulation of the cellular activation threshold is another mechanism underlying cofactor-induced anaphylaxis, most likely also functional when infectious diseases orchestrate elicitation of anaphylaxis. Cofactors are increasingly accepted to play a fundamental role in eliciting anaphylaxis. Consequently, to improve patient management modalities, a better understanding of the underlying mechanisms is warranted. This review aims to update clinicians and clinical scientists on recent developments.

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Section: Figurementioning

confidence: 99%

About the role and underlying mechanisms of cofactors in anaphylaxis

Wölbing

Fischer

Köberle

et al. 2013

Allergy

178

163

View full text Add to dashboard Cite

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“…Palosuo et al (26,27) identified -5 gliadin, a component of fast -gliadin, as a major allergen in WDEIA. They revealed that the -5 gliadin is also an allergen in children with an immediate-type allergy to wheat (27) and that transglutaminase-mediated cross-linking of a pepsintrypsin-digested -5 gliadin causes a marked increase in IgEbinding both in vitro and in vivo (28). In this study, we analyzed the linear IgE-binding epitopes of -5 gliadin by using synthetic peptides.…”

mentioning

confidence: 99%

Identification of the IgE-binding Epitope in ω-5 Gliadin, a Major Allergen in Wheat-dependent Exercise-induced Anaphylaxis

Matsuo

Morita

Tatham

et al. 2004

Journal of Biological Chemistry

167

186

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Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a severe IgE-mediated allergic reaction provoked by the combination of wheat-ingestion with intensive physical exercise over the next few hours. Among wheat proteins, -5 gliadin, which is one of the components of fast -gliadin, has been reported as a major allergen in the anaphylaxis. In this study, we detected IgE-binding epitopes within the primary sequence of -5 gliadin using arrays of overlapping peptides synthesized on derivatized cellulose membranes. Sera from four patients with WDEIA having specific IgE to the fast -gliadin were used to probe the membrane. Seven epitopes, QQIPQQQ, QQLPQQQ, QQFPQQQ, QQSPEQQ, QQSPQQQ, QQYPQQQ, and PYPP, were detected within the primary sequence of -5 gliadin. By using sera of 15 patients, 4 of them, QQIPQQQ, QQFPQQQ, QQSPEQQ, and QQSPQQQ, were found to be dominant epitopes. Mutational analysis of the QQIPQQQ and QQFPQQQ indicated that amino acids at positions Gln 1 , Pro 4 , Gln 5 , Gln 6 , and Gln 7 were critical for IgE binding. These results will provide a useful tool for developing safer wheat products in addition to diagnostic and immunotherapy techniques for WDEIA.Food-dependent exercise-induced anaphylaxis is a distinct form of food allergy induced by physical exercise (1). Food items such as shrimp (2), hazelnut (3), buckwheat (4), corn (5), and celery (6) are responsible for the development of food-dependent exercise-induced anaphylaxis. However, of all of the various kinds of food, wheat is reported to be the allergen with the highest frequency in Japan (7). Symptoms are typically generalized urticaria and severe allergic reactions such as shock or hypotension. Because of this serious reaction, it is important to determine the causative food to avoid the allergic reaction. A challenge test consisting of ingestion of the assumed food followed by intense physical exercise is the only reliable method to determine the causative food and to diagnose the disease. However, the challenge test is not always safe because in some cases the test induces an anaphylactic shock. In addition, the most reliable treatment for this disease is to avoid taking the causative food or, alternatively, to take a rest after meals. However, in the case of wheat allergy, elimination causes a decline in the quality of life for the patients. Thus, an in vitro diagnostic method as well as hypoallergenic wheat is necessary for patients with wheat-dependent exercise-induced anaphylaxis (WDEIA). 1Recent studies have revealed the IgE-binding epitopes of several food allergens including egg (8), milk (9, 10), soybean (11), and peanut (12), whereas the IgE-binding epitopes for wheat allergen are controversial. Wheat protein is composed of water/salt-soluble proteins and water/salt-insoluble proteins. Proteins in the water/salt-soluble fraction, such as ␣-amylase inhibitor, peroxidase, glycerinaldehyde-3-phosphate dehydrogenase, serpin, and triosephosphate isomerase, have been considered to be major allergens in patients with bakers' asthma (13-15...

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“…The activation of transglutaminase is associated with the release of histamine from the cells induced by an IgE-dependent mechanism (2). Transglutaminase-mediated cross-linking of a peptic fraction of -5 gliadin enhances IgE reactivity in wheat-dependent, exercise-induced anaphylaxis (3). TGaseII is overexpressed in asthmatic airways and functions to increase sPLA(2)-X enzymatic activity and acts as a key initiator of the airway inflammatory cascade in asthma (6).…”

mentioning

confidence: 99%

“…Transglutaminase II (TGaseII) 3 is a protein cross-linking enzyme with diverse functions (1)(2)(3)(4)(5). The activation of transglutaminase is associated with the release of histamine from the cells induced by an IgE-dependent mechanism (2).…”

mentioning

confidence: 99%

Transglutaminase II/MicroRNA-218/-181a Loop Regulates Positive Feedback Relationship between Allergic Inflammation and Tumor Metastasis

Eom

Kim

et al. 2014

Journal of Biological Chemistry

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Background:The mechanism of transglutaminase II-mediated allergic inflammation-promoted tumor metastasis remains unknown at the molecular level. Results: The transglutaminase II/miR-218/-181a loop regulates allergic inflammation-promoted tumor metastasis. Conclusion: Transglutaminase II mediates a positive feedback between allergic inflammation and tumor metastasis. Significance: Transglutaminase II can be a target for the development of allergy and cancer therapeutics.

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Transglutaminase-mediated cross-linking of a peptic fraction of ω-5 gliadin enhances IgE reactivity in wheat-dependent, exercise-induced anaphylaxis

Cited by 143 publications

References 42 publications

About the role and underlying mechanisms of cofactors in anaphylaxis

About the role and underlying mechanisms of cofactors in anaphylaxis

Identification of the IgE-binding Epitope in ω-5 Gliadin, a Major Allergen in Wheat-dependent Exercise-induced Anaphylaxis

Transglutaminase II/MicroRNA-218/-181a Loop Regulates Positive Feedback Relationship between Allergic Inflammation and Tumor Metastasis

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