Transgressive segregation for reduced glucosinolate content in Brassica carinata A. Braun

Abstract

Successful commercial utilization of the meal by-product of Brassica oilseed crops requires the cultivation of cultivars with low glucosinolate (GSL) content in the seeds; however, such cultivars are not yet available in Brassica carinata. The objective of the present research was to search for transgressive segregants with further-reduced GSL content in the progeny of crosses involving four B. carinata lines with reduced GSL content (90 compared with 120 lmol/g seed in standard germplasm). The four lines were crossed following a diallel design and F 2 phenotypes (F 3 seed bulked) were analysed for GSL content. F 2 phenotypes with a transgressive GSL content lower than the parents were identified in all crosses involving the line S2-1241, suggesting that this line carries alleles for reduced GSL content not present in the other lines. F 3 : 4 lines from transgressive F 2 phenotypes were evaluated for 2 years, which resulted in the selection of an F 3 : 4 line with an average GSL content of 58 and 46 lmol/g seed, respectively compared with 84 and 62 lmol/g seed, respectively in S2-1241.

Alterations in the leaf lipidome of Brassica carinata under high-temperature stress

“…Its ability to reduce summer weed species’ seed banks makes it suitable for integrated pest management in crop production systems [ 7 , 16 ]. Carinata also has potential as a feed crop (due to its high protein and low fiber content [ 17 – 19 ]) and biofumigant (varieties with high glucosinolate content [ 20 ]). There have been concerted efforts both from public and private entities in the last few years to establish carinata as a sustainable biofuel crop in the United States [ 1 , 16 , 21 – 24 ].…”

Development and characterisation of a Brassica carinata inbred line incorporating genes for low glucosinolate content from B. juncea

“…The task seems arduous, but it will only possible if approached through several strategies. Previous research conducted through intraspecific selection led to the development of a line with stable glucosinolate content of 52 micromoles g -1 seed (Márquez-Lema et al 2006). Recombination between that genetic source and the line BCH-1773 developed in the present research seems an attractive approach that might result in a further improvement of the trait.…”

“…Velasco et al (1999) reported the development of six lines with a slight reduced glucosinolate content (80-100 micromoles g -1 seed compared to 120 micromoles g -1 seed in the check). Crosses between these lines produced transgressive segregation from which a line with a glucosinolate content averaged over two environments of 52 micromoles g -1 seed, compared to 73 micromoles g -1 seed in the best parent, was isolated (Márquez-Lema et al 2006). The present research represents a further advance in the reduction of glucosinolate content in B. carinata.…”

“…Such a reduction was proved to be consistent over environments. In crosses involving the abovementioned lines with reduced glucosinolate content, Márquez-Lema et al (2006) isolated a transgressive line with average glucosinolate contents in two environments of 58 and 46 micromoles g -1 , respectively compared to 84 and 62 micromoles g -1 , respectively in the seeds of the parent with the lowest glucosinolate content. Despite some success in reducing glucosinolate content through intraspecific breeding, the reported levels as still far from the low levels available in the other Brassica oilseed crops.…”

Glucosinolates: Natural Occurrence, Biosynthesis, Accessibility, Isolation, Structures, and Biological Activities

“…Breeding approaches are often employed to obtain crops with low GSL content for food or feed purposes [ 26 , 27 , 28 ], while those with high GSL content remain of interest for non-food applications. Therefore, the choice of species should be carefully considered with regard to the downstream purposes of raw materials.…”