Transient Cold Shock Induces Oxidative Stress Events in Antarctic Fungi

Kostadinova, Nedelina; Krumova, Ekaterina; Stefanova, Tzvetanka; Dishliyska, Vladislava; Angelova, Maria

doi:10.5772/35127

Cited by 11 publications

(10 citation statements)

References 75 publications

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“…In previous studies, we have identified coldactive CAT producers belonging to the genera Aspergillus, Cladosporium, and Penicillium among fungi recovered from Antarctica [23,34]. Our experiments have also demonstrated enhanced CAT activity in Antarctic fungi grown under cold stress conditions [35,36]. All of these studies reported only the CAT distribution among fungal strains isolated from permanently cold habitats.…”

Section: •−mentioning

confidence: 49%

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

et al. 2021

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Cold-active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold-active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme production was achieved by optimization of medium and culture conditions. Maximum CAT was demonstrated under low glucose content (2%), 10% inoculum size, temperature 20°C, and dissolved oxygen concentration (DO) 40%. An effective laboratory technology based on changing the oxidative stress level through an increase of DO in the bioreactor was developed. The used strategy resulted in a 1.7-and 1.4-fold enhanced total enzyme activity and maximum enzyme productivity. The enzyme was purified and characterized. P. griseofulvum P29 CAT was most active at approximately 20°C and pH 6.0. Its thermostability was in the range between 5°C and 40°C.

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Section: •−mentioning

confidence: 49%

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

et al. 2021

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“…Besides the transient character of the cold stress, the enhancement of SOD activity was observed until the end of cultivation. Our previous studies have demonstrated that the increased level of ROS generated during the temperature downshift induced SOD biosynthesis in A. glaucus 363 (Kostadinova et al 2012). Similar approach has been used for improving SOD activity in different pro-and eukaryotic organisms (Smirnova et al 2001;Baek & Skinner 2012;Kayihan et al 2012;Xu et al 2013).…”

Section: Discussionmentioning

confidence: 94%

“…In a previous paper we reported the presence of three SOD isoenzymes in the cell-free extract from A. glaucus 363 (Kostadinova et al 2012). They were designated as Mn-SOD, Cu/Zn-SODI, and Cu/Zn-SODII in the order of their migration towards the anode.…”

Section: Purification Of Aspergillus Glaucus Sodsmentioning

confidence: 97%

“…During the course of previous investigation on Antarctic fungi, a high level of CA SOD in Aspergillus glaucus 363 was detected (Tosi et al 2010). Furthermore, short-term treatment of exponentially growing culture of A. glaucus 363 with low temperatures enhanced the level of oxidative stress that resulted in the activation of antioxidant enzyme defence (Kostadinova et al 2012). Based on the above mentioned findings, a transient cold-stress was applied in order to increase the yield of the first antioxidant enzyme SOD.…”

Section: Production Of Ca Sodmentioning

confidence: 99%

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Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363

et al. 2016

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The Antarctic fungal strain Aspergillus glaucus 363 produces cold-active (CA) Cu/Zn-superoxide dismutase (SOD). The strain contains at least one gene encoding Cu/Zn-SOD that exhibited high homology with the corresponding gene of other Aspergillus species. To our knowledge, this is the first nucleotide sequence of a CA Cu/Zn-SOD gene in fungi. An effective laboratory technology for A. glaucus SOD production in 3 L bioreactors was developed on the basis of transient cold-shock treatment. The temperature downshift to 10 °C caused 1.4-fold increase of specific SOD activity compared to unstressed culture. Maximum enzyme productivity was 64 × 10(3) U kg(-1) h(-1). Two SOD isoenzymes (Cu/Zn-SODI and Cu/Zn-SODII) were purified to electrophoretic homogeneity. The specific activity of the major isoenzyme, Cu/Zn-SODII, after Q-Sepharose chromatography was 4000 U mg(-1). The molecular mass of SODI (38 159 Da) and of SODII (15 835 Da) was determined by electrospray quadropole time-of-flight (ESI-Q-TOF) mass spectrometry and dynamic light scattering (DLS). The presence of Cu and Zn were confirmed by inductively coupled plasma mass spectrometry (ICP-MS). The N-terminal amino acid sequence of Cu/Zn-SODII revealed a high degree of structural homology with Cu/Zn-SOD from other fungi, including Aspergillus species.

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“…The increase in glycogen and trehalose levels in fungal response to adverse environmental conditions has been recorded as a cellular response to induced oxidative stress . Furthermore, the prevalence of heavy metals in the living environment instigates oxidative stress .…”

Section: Resultsmentioning

confidence: 99%

Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

Krumova

Kostadinova

Miteva‐Staleva

et al. 2016

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The fungal strain Aspergillus fumigatus 3 2 isolated from the tailings pond at the copper mine "Vlaykov vruh," Bulgaria, showed high tolerance to Cu 2þ and Zn 2þ . This study was designed to explore the relationship between Cu 2þ and Zn 2þ tolerance and cellular response to oxidative stress. The model strain was identified to species level by morphological and molecular methods. Fungal cultures were exposed to enhanced concentrations of metal ions. The effect of Cu 2þ and Zn 2þ was evaluated by the changes in fungal growth and morphology, the level of oxidative stress biomarkers, and the antioxidant activities of enzymes such as superoxide dismutase (SOD) and catalase (CAT). Two different cellular responses occurred: The concentrations of up to 500 mg/mL caused enhanced levels of oxidative stress biomarkers (glycogen and trehalose accumulation and oxidatively damaged protein content), as well as an increase in SOD and CAT activities. The treatment with concentrations from 500 to 2000 mg/mL led to enhanced glycogen consumption, accelerated proteolysis, and a decrease in SOD and CAT activities. The present results provide additional information about the participation of oxidative stress and antioxidant defense in enhanced tolerance of fungi isolated from metal-polluted soils. Probably, survival at extremely high concentrations also requires the participation of other defense mechanisms. Furthermore, the enhanced understanding of these processes will provide essential tools for efficient remediation practices.

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Transient Cold Shock Induces Oxidative Stress Events in Antarctic Fungi

Cited by 11 publications

References 75 publications

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum

Production, purification, and characterization of a novel cold-active superoxide dismutase from the Antarctic strain Aspergillus glaucus 363

Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

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