Transient concentration of a γ‐tubulin‐related protein with a pericentrin‐related protein in the formation of basal bodies and flagella during the differentiation of <i>Naegleria gruberi</i>

Suh, Mi Ra; Han, Ji Yun; No, Yi Ran; Lee, Joohun

doi:10.1002/cm.10033

Cited by 18 publications

(34 citation statements)

References 55 publications

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“…Additionally, we observed that ␥-tubulin is consistently localized to the basal bodies throughout differentiation. This result is in direct contrast to what has been previously described (32). We propose that the previous reports of Naegleria ␥-tubulin foci moving to the opposite end of the cell from the MTOC after basal body assembly were likely an artifact of using heterologous antibodies.…”

Section: Discussioncontrasting

confidence: 99%

“…Naegleria's genome encodes two centrins: the previously characterized centrin (which we followed in this study) (19) that is a homolog of human centrin-1, and a second centrin that represents a homolog of human centrin-3 and yeast Cdc31p. Surprisingly, we did not find evidence of a homolog of pericentrin, despite a report of localization of this protein in Naegleria using heterologous antibodies (32). The presence of many known basal body genes indicates that although Naegleria's basal bodies are transient structures, they are likely canonical in composition as well as structure.…”

Section: Resultscontrasting

confidence: 78%

“…␥-Tubulin is a general microtubule nucleation factor that localizes to microtubule-organizing centers (MTOCs) of many types. Surprisingly, Naegleria's ␥-tubulin homolog has been reported to localize to basal body precursor complexes and then move to the other end of the cell before disappearing completely (32).…”

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confidence: 99%

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Naegleria gruberi De Novo Basal Body Assembly Occurs via Stepwise Incorporation of Conserved Proteins

et al. 2010

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Centrioles and basal bodies are discrete structures composed of a cylinder of nine microtubule triplets and associated proteins. Metazoan centrioles can be found at mitotic spindle poles and are called basal bodies when used to organize microtubules to form the core structure of flagella. Naegleria gruberi, a unicellular eukaryote, grows as an amoeba that lacks a cytoplasmic microtubule cytoskeleton. When stressed, Naegleria rapidly (and synchronously) differentiates into a flagellate, forming a complete cytoplasmic cytoskeleton de novo, including two basal bodies and flagella. Here, we show that Naegleria has genes encoding conserved centriole proteins. Using novel antibodies, we describe the localization of three centrosomal protein homologs (SAS-6, ␥-tubulin, and centrin-1) during the assembly of the flagellate microtubule cytoskeleton. We also used these antibodies to show that Naegleria expresses the proteins in the same order as their incorporation into basal bodies, with SAS-6 localizing first, followed by centrin and finally ␥-tubulin. The similarities between basal body assembly in Naegleria and centriole assembly in animals indicate that mechanisms of assembly, as well as structure, have been conserved throughout eukaryotic evolution.

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Section: Discussioncontrasting

confidence: 99%

Section: Resultscontrasting

confidence: 78%

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Naegleria gruberi De Novo Basal Body Assembly Occurs via Stepwise Incorporation of Conserved Proteins

et al. 2010

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“…These cells are, therefore, at beginning of differentiation when the first centrioles start to be detected by EM analysis. The initial events that occur during de novo centriole formation in Mastotermes spermatids are, therefore, very similar to the events described during the de novo formation of centrioles in HeLa cells, in which original centrioles have been removed, [La Terra et al, 2005] and during basal body assembly in Naegleria gruberi [Suh et al, 2002], where cytoplasmic foci of g-tubulin are seen before centriole appearance. As spermatid differentiation proceeds in Mastotermes the pericentrin staining concentrates near the basal region of the nucleus in correspondence of the cytoplasmic domain where centriole formation occurs.…”

Section: Discussion the Unique Multiflagellated Sperm In Insectsmentioning

confidence: 85%

Centrioles to basal bodies in the spermiogenesis of Mastotermes darwiniensis (Insecta, Isoptera)

Riparbelli

Callaini

Mercati

et al. 2009

Cell Motil. Cytoskeleton

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In addition to their role in centrosome organization, the centrioles have another distinct function as basal bodies for the formation of cilia and flagella. Centriole duplication has been reported to require two alternate assembly pathways: template or de novo. Since spermiogenesis in the termite Mastotermes darwiniensis lead to the formation of multiflagellate sperm, this process represents a useful model system in which to follow basal body formation and flagella assembly. We present evidence of a possible de novo pathway for basal body formation in the differentiating germ cell. This cell also contains typical centrosomal proteins, such as centrosomin, pericentrin-like protein, gamma-tubulin, that undergo redistribution as spermatid differentiation proceeds. The spermatid centrioles are long structures formed by nine doublet rather than triplet microtubules provided with short projections extending towards the surrounding cytoplasm and with links between doublets. The sperm basal bodies are aligned in parallel beneath the nucleus. They consist of long regions close to the nucleus showing nine doublets in a cartwheel array devoid of any projections; on the contrary, the short region close to the plasma membrane, where the sperm flagella emerge, is characterized by projections similar to those observed in the centrioles linking the basal body to the plasma membrane. It is hypothesized that this appearance is in connection with the centriole elongation and further with the flagellar axonemal organization. Microtubule doublets of sperm flagellar axonemes are provided with outer dynein arms, while inner arms are rarely visible.

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“…Cell fixation and immunocytochemistry were performed as described previously (Han et al, 1997;Suh et al, 2002).…”

Section: Cell Fixation and Immunocytochemistrymentioning

confidence: 99%

NgUNC-119, Naegleria homologue of UNC-119, localizes to the flagellar rootlet

Chung

Kang

Paik

et al. 2007

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Transient concentration of a γ‐tubulin‐related protein with a pericentrin‐related protein in the formation of basal bodies and flagella during the differentiation of Naegleria gruberi

Abstract: The distribution of two proteins in Naegleria gruberi, N-␥TRP (Naegleria ␥-tubulin-related protein) and N-PRP (Naegleria pericentrin-related protein), was examined during the de novo formation of basal bodies and flagella that occurs during the differentiation of N. gruberi.

Cited by 18 publications

References 55 publications

Naegleria gruberi De Novo Basal Body Assembly Occurs via Stepwise Incorporation of Conserved Proteins

Naegleria gruberi De Novo Basal Body Assembly Occurs via Stepwise Incorporation of Conserved Proteins

Centrioles to basal bodies in the spermiogenesis of Mastotermes darwiniensis (Insecta, Isoptera)

NgUNC-119, Naegleria homologue of UNC-119, localizes to the flagellar rootlet

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