Transient expression of collagen type XIV during muscle development and its reappearance after denervation and degeneration.

Abstract

In the formation of muscle pattern, the architectural arrangement is believed to be controlled by the local connective tissue cells. In this study we examined the immunohistological localization of Type XIV collagen recognized by a monoclonal antibody, MAb DBM, in embryonic chick hind limbs from stage (St.) 27 to 2 weeks post hatching. DBM staining was transiently observed in the epimysium from St. 30, in the perimysium of the dorsal region from St. 37, and in the entire perimysium from St. 39. After hatching, DBM staining was notably diminished in both epimysium and perimysium. In contrast, DBM staining and in situ hybrid-

Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles

“…At a higher magnification EphA3 was clearly expressed by the muscle connective tissue, perimysium, but not by the myotubes ( Fig. 9N,O and schema in Tono‐oka et al 1996 ), consistent with the origin of the connective tissues.…”

“…Furthermore, EphA3 or EphA4 expressing motor axons innervated the same receptor expressing hindlimb muscles as has been suggested in the case of EphA3 and EphA3‐ positive axial muscles ( Kilpatrick et al 1996 ). Muscle connective tissues are considered to function in muscle patterning ( Tono‐oka et al 1996 ) and also in intramuscular nerve formation ( Phelan & Hollyday 1990). As Eph receptor–receptor interactions are unlikely, myoblasts and myotubes presumably express some Eph ligands and interact with surrounding connective tissues and motor axons.…”

Genes and chromosomal breakpoints in the Langer-Giedion syndrome region on human chromosome 8

“…Hyaluronan is a high molecular weight GAG that is synthesized at the inner face of the plasma membrane and extruded to the outside of the cell. It is considered to play roles in muscle development and regeneration (Tono-Oka et al 1996). DAP-β-A1 is part of a large oligomeric complex of dystrophin-associated membrane glycoproteins.…”

Dorsoventral differential distribution of collagen type XIV around the spinal cord is regulated by the ectoderm

“…This molecule could be used for a dorsal marker of the spinal cord ( Hirano & Tanaka 1994). The antigen recognized by mAb DBM was found to be collagen type XIV ( Tono‐oka et al 1996 ). In the present study we found that the expression of collagen type XIV around the spinal cord was regulated by the ectoderm and that the ventralizing tissues, notochord and floor plate began to express the molecule on their basement membrane when they were transplanted near the ectoderm.…”