2014
DOI: 10.1016/j.scienta.2014.10.002
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Transient expression of siRNA targeted against the TYLCV AV1, AC1 and AC3 genes for high resistance in tomato

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Cited by 9 publications
(8 citation statements)
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“…Tomato expression vectors pBIN438-AV1 (i/r), pBIN438-AC1(i/r), pBIN438-AC3(i/r) and pBIN438-AV1-AC1-AC3(i/r) were constructed previously in our laboratory. [9] The expression of the gene of interest was under the control of the double constitutive CaMV 35S promoter ( Figure 1). The plasmid was transferred into the competent cells of A. tumefaciens LBA4404 through the freezeÀthaw method.…”
Section: Bacterial Strain and Plant Expression Vector Constructionmentioning
confidence: 99%
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“…Tomato expression vectors pBIN438-AV1 (i/r), pBIN438-AC1(i/r), pBIN438-AC3(i/r) and pBIN438-AV1-AC1-AC3(i/r) were constructed previously in our laboratory. [9] The expression of the gene of interest was under the control of the double constitutive CaMV 35S promoter ( Figure 1). The plasmid was transferred into the competent cells of A. tumefaciens LBA4404 through the freezeÀthaw method.…”
Section: Bacterial Strain and Plant Expression Vector Constructionmentioning
confidence: 99%
“…[9] Untransformed tomato was selected as a negative control, whereas miRNA 166 was applied as a loading control. [9] The gels were blotted overnight on Hybond-N C membrane (Roche). The membrane was hybridized with PCR-labelled gene-specific Digoxin (DIG) probes (Roche).…”
Section: Northern Blot Analysismentioning
confidence: 99%
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