2014
DOI: 10.4149/av_2014_03_274
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Transient expression of the influenza A virus PB1-F2 protein using a plum pox virus-based vector in Nicotiana benthamiana

Abstract: Summary. -PB1-F2 protein of influenza A virus (IAV) was cloned in a plum pox virus (PPV) genome-based vector and attempts to express it in biolistically transfected Nicotiana benthamiana plants were performed. The vector-insert construct replicated in infected plants properly and was stable during repeated passage by mechanical inoculation, as demonstrated by disease symptoms and immunoblot detection of PPV capsid protein, while PB1-F2-specific band was more faint. We showed that it was due its low solubility.… Show more

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Cited by 6 publications
(3 citation statements)
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“…1a). Similar behavior has been recently described for in planta expressed PB1-F2 protein of infl uenza virus, known to create insoluble amyloid fi bres (Kamencayová et al, 2014). Th erefore we presume P1-his low solubility either as its intrinsic feature, or as a result of its affi nity to some plant compound sedimenting with the debris.…”
supporting
confidence: 78%
“…1a). Similar behavior has been recently described for in planta expressed PB1-F2 protein of infl uenza virus, known to create insoluble amyloid fi bres (Kamencayová et al, 2014). Th erefore we presume P1-his low solubility either as its intrinsic feature, or as a result of its affi nity to some plant compound sedimenting with the debris.…”
supporting
confidence: 78%
“…Due to the increasing interest in organic farming to reduce the use of synthetic chemicals in agriculture, viral-based vector to manage crop pests and diseases in a sustainable way is a good strategy. A virus-mediated transgenic system is a quick system to deliver genes without any need for transformation and regeneration techniques and has currently been successfully used in apples and other woody species [33][34][35][36][37][38][39][40][41][42]. More efforts need to be made to discover new viruses in mulberry to accelerate CLBV-ML2 the application of virus-mediated transgenic in mulberry varieties.…”
Section: Discussionmentioning
confidence: 99%
“…It is a proper propagative host also for viruses naturally infecting perennial plants, such as plum pox virus (PPV). We have extensively used this artifi cial host plant species, e. g. for multiplication of emerging PPV strains, as primary target for developing biolistic technique, for analysis of PPV strain competitiveness and of mutations accumulated in the PPV genome, or for expression of foreign genes cloned in the PPV genome (Glasa et al, 2004;Predajňa et al, 2010;Šubr et al, 2012;Kamencayová et al, 2014). Here we attempt to compare the transcripts from healthy and PPV-infected N. benthamiana plants to reveal the regulatory eff ects in the course of systemic viral infection.…”
Section: Introductionmentioning
confidence: 99%