Quantitative relations between the softening mechanism and the constitutive model are determined based on the dislocation density, the influences of various deformation parameters, the dynamic softening mechanism, and static softening mechanism. A unified explicit constitutive model of multipass deformation is established by coupling the microstructural model of Q345B steel (the equivalent of ASTM A572Gr50). The flow stress curves under different deformation conditions are obtained from a Gleeble-3500 thermosimulation machine during hot compression deformation, including single-pass and double-pass compression for Q345B. From the experimental results, a new method to identify parameters is developed to determine the material parameters for the unified constitutive model and to establish their analytic expressions. The results predicted from the proposed model are also presented for validation and compared with the experimental results. The proposed model can accurately describe the stressstrain curves during dynamic recovery on the high-stress stage, during dynamic recrystallization, steady flow state, and next pass after the completion of static softening. In addition, the errors from the model are adequately analyzed to make further improvements.