Transient Protein Interactions between Cytochrome <i>f</i> and the Phytocyanin Umecyanin

Sato, Katsuko; Crowley, Peter B.; Dennison, Christopher

doi:10.1002/cbic.200700071

Cited by 2 publications

(1 citation statement)

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“…UCLs are members of the ancient and plant-specific phytocyanin family, which is a subfamily of blue copper proteins (Nersissian et al, 1998;Ma et al, 2011). Thus far, most of the research on phytocyanins has been on their biochemical characteristics and redox properties (Sato et al, 2007;Guzzi et al, 2008;Ma et al, 2011;Cao et al, 2015). Recent studies showed that phytocyanin is a pollen tube growthcontrolling protein (Dong et al, 2005).…”

Section: Subcellular Localization Of Osucl8 Protein and Its Effects Omentioning

confidence: 99%

MiR408 Regulates Grain Yield and Photosynthesis via a Phytocyanin Protein

et al. 2017

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Increasing grain yield is the most important object of crop breeding. Here, we report that the elevated expression of a conserved microRNA, OsmiR408, could positively regulate grain yield in rice () by increasing panicle branches and grain number. We further showed that OsmiR408 regulates grain yield by down-regulating its downstream target, , which is an uclacyanin (UCL) gene of the phytocyanin family. The knock down or knock out of also increases grain yield, while the overexpression of results in an opposite phenotype. Spatial and temporal expression analyses showed that was highly expressed in pistils, young panicles, developing seeds, and inflorescence meristem and was nearly complementary to that of OsmiR408. Interestingly, the OsUCL8 protein was localized to the cytoplasm, distinct from a majority of phytocyanins, which localize to the plasma membrane. Further studies revealed that the cleavage of by miR408 affects copper homeostasis in the plant cell, which, in turn, affects the abundance of plastocyanin proteins and photosynthesis in rice. To our knowledge, this is the first report of the effects of miR408- in regulating rice photosynthesis and grain yield. Our study further broadens the perspective of microRNAs and UCLs and provides important information for breeding high-yielding crops through genetic engineering.

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