Transient response of continuous cultures to changes in temperature

Ryu, Du Yeol; Máteles, R. I.

doi:10.1002/bit.260100311

Cited by 29 publications

(1 citation statement)

References 11 publications

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“…On the other hand, according to the transient study carried out by Ryu and Mateles (1968), the growth rate changes rapidly after the temperature change in continuous culture. When the temperature was changed from 33°C to 37"C, the functioning ribosome was converted to the nonfunctioning ribosome due to the increase in translational activity of ribosome, and the functioning ribosome content decreased.…”

Section: Synthesis Of Ribosome During Temperature Shift-up and Shift-mentioning

confidence: 99%

Regulation of ribosome synthesis in Escherichia coli: Effects of temperature and dilution rate changes

Yun

Hong

Lim

2000

Biotechnol. Bioeng.

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The effect of temperature on the synthesis of ribosome in Escherichia coli K‐12 was investigated. In continuous fermentation, the total and functioning ribosome contents decreased with increasing temperature, while the non‐functioning ribosome content remained unchanged. Cells contained higher amounts of functioning ribosome at lower temperatures to compensate for the decrease in translational activity. A transient study was performed to investigate the dynamic response of the cell to changes in the dilution rate. In response to the dilution rate shift‐up, the cell mass decreased until the cells produced a sufficient amount of ribosomes to support the new higher growth rate. However, the response to the dilution rate shift‐down resulted in an immediate increase in cell mass. This may be due to the fact that the cell already contains enough ribosomes to support a lower growth rate corresponding to the new low dilution rate. Based on the experimental results, a mathematical model was developed to describe the cell growth at transient as well as steady states. The total ribosome content was included as a variable because it affects the growth rate of the cell. © 1996 John Wiley & Sons, Inc.

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Section: Synthesis Of Ribosome During Temperature Shift-up and Shift-mentioning

confidence: 99%

Regulation of ribosome synthesis in Escherichia coli: Effects of temperature and dilution rate changes

Yun

Hong

Lim

2000

Biotechnol. Bioeng.

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Continuous culture of Pseudomonas fluorescens with sodium maleate as a carbon source

Edwards

Kinsella

Sholiton

1972

Biotech & Bioengineering

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Pseudomonas fluorescens (ATCC 11150) was grown in batch and continuous culture in minimal media with sodium maleate as growth-limiting sole organic carbon source. Growth was followed by turbidity and dry weight measurements. Gross composition of washed cells (relative amounts of protein, lipid, RNA, and DNA) and the distribution of amino acids in protein hydrolysates of the cells were determined for cells grown in continuous culture a t various dilution rates. Extracellular concentrations of the original carbon source and a number of metabolites were monitored by a total carbon analysis, ion exchange chromatography, and ultraviolet-visible scans of cell-free supernatants and chromatographic fractions, thereof.Substrate inhibition by maleate was a major factor in the growth kinetics of both batch and continuous cultures. Excessive maleate concentration caused instability in continuous cultures. By appropriate operation, much higher specific growth rates (0.305/hr) could ultimately be achieved in continuous culture compared to batch culture (0.174/hr). Adaptation was responsible for only part of the differences between batch and continuous cultures; the differing distribution of metabolites were also major factors.

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Growth models of cultures with two liquid phases. VII. Substrate dissolved in dispersed phase; effect of dispersed phase volume and temperature

Prokop

Erickson²

1972

Biotech & Bioengineering

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SummaryThe effects of dispersed phase volume and temperature on the batch growth of Cundida Zipolytica on gas oil are investigated. Growth parameters are presented for two sets of experiments. The shape of growth curves was basically similar to the system composed of n-hexadecane dissolved in dewaxed gas oil, in spite of the complex nature of the substance. All of the batch growth curves exhibited a linear growth region. The rate of linear growth and its length varied with change in dispersed phase volume. The effect of temperature on growth rate was investigated for temperatures ranging from 23°C to 34°C. The results show a smaller activation energy during linear growth than during the early stages of batch growth. These results are analyzed from the viewpoint of growth models presented previously. The results indicate that growth at drop surfaces is important and that segregation effects may be important.

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Transient response of continuous cultures to changes in temperature

Cited by 29 publications

References 11 publications

Regulation of ribosome synthesis in Escherichia coli: Effects of temperature and dilution rate changes

Regulation of ribosome synthesis in Escherichia coli: Effects of temperature and dilution rate changes

Continuous culture of Pseudomonas fluorescens with sodium maleate as a carbon source

Growth models of cultures with two liquid phases. VII. Substrate dissolved in dispersed phase; effect of dispersed phase volume and temperature

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