Transient‐state kinetic analysis of complex formation between photoprotein clytin and <scp>GFP</scp> from jellyfish <i>Clytia gregaria</i>

Eremeeva, Elena V.; Berkel, Willem J.H. van; Vysotski, Eugene S.

doi:10.1002/1873-3468.12052

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…It was proposed that, upon Ca 2+ binding, clytin adopts a conformation with several orders of magnitude enhanced affinity for cgGFP; then, a very transient complex is formed, in which chemiexcitation of clytin and subsequent energy transfer to cgGFP occur. In other studies, no stable complex of Ca 2+ discharged clytin with cgGFP has been detected, suggesting that such BRET complex must indeed be a short-lived intermediate in the clytin bioluminescence process (54,57).…”

Section: From Natural Bret To Designed Fp-photoprotein Fusionsmentioning

confidence: 84%

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

Bakayan

Domingo

Vaquero

et al. 2017

Photochem & Photobiology

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Calcium‐activated photoproteins, such as aequorin, have been used as luminescent Ca2+ indicators since 1967. After the cloning of aequorin in 1985, microinjection was substituted by its heterologous expression, which opened the way for a widespread use. Molecular fusion of green fluorescent protein (GFP) to aequorin recapitulated the nonradiative energy transfer process that occurs in the jellyfish Aequorea victoria, from which these two proteins were obtained, resulting in an increase of light emission and a shift to longer wavelength. The abundance and location of the chimera are seen by fluorescence, whereas its luminescence reports Ca2+ levels. GFP‐aequorin is broadly used in an increasing number of studies, from organelles and cells to intact organisms. By fusing other fluorescent proteins to aequorin, the available luminescence color palette has been expanded for multiplexing assays and for in vivo measurements. In this report, we will attempt to review the various photoproteins available, their reported fusions with fluorescent proteins and their biological applications to image Ca2+ dynamics in organelles, cells, tissue explants and in live organisms.

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Section: From Natural Bret To Designed Fp-photoprotein Fusionsmentioning

confidence: 84%

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

Bakayan

Domingo

Vaquero

et al. 2017

Photochem & Photobiology

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“…However, a recombinant aequorin–GFP fusion protein does generate the same green GFP emission as in vivo . Stopped‐flow experiments, one using this fusion protein as well more recently, clytin and Clytia GFP in free solution, revealed that in the course of the <10 ms rise time of the bioluminescence after Ca 2+ addition, the efficiency of bioluminescence FRET measured by the 510/470 nm intensity ratio increased at the same rate as the rise of bioluminescence intensity . This intriguing phenomenon of “GFP‐FRET” is a rich subject for a productive physical–chemical study (… the “Endless Frontier”).…”

Section: Firefly and Coelenterazinementioning

confidence: 91%

Perspectives on Bioluminescence Mechanisms

Lee

2016

Photochem & Photobiology

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The molecular mechanisms of the bioluminescence systems of the firefly, bacteria and those utilizing imidazopyrazinone luciferins such as coelenterazine are gradually being uncovered using modern biophysical methods such as dynamic (ns-ps) fluorescence spectroscopy, NMR, X-ray crystallography and computational chemistry. The chemical structures of all reactants are well defined, and the spatial structures of the luciferases are providing important insight into interactions within the active cavity. It is generally accepted that the firefly and coelenterazine systems, although proceeding by different chemistries, both generate a dioxetanone high-energy species that undergoes decarboxylation to form directly the product in its S 1 state, the bioluminescence emitter. More work is still needed to establish the structure of the products completely. In spite of the bacterial system receiving the most research attention, the chemical pathway for excitation remains mysterious except that it is clearly not by a decarboxylation. Both the coelenterazine and bacterial systems have in common of being able to employ "antenna proteins," lumazine protein and the greenfluorescent protein, for tuning the color of the bioluminescence. Spatial structure information has been most valuable in informing the mechanism of the Ca 2+ -regulated photoproteins and the antenna protein interactions.

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“…In this bioluminescent-fluorescent system GFP shifts the blue light produced by Aequorin to longer wavelengths, i.e. green light (Eremeeva, Van Berkel & Vysotski, 2016). Various bioluminescent-fluorescent systems have been developed in jellyfish (Fourrage et al, 2014).…”

Section: Functions Of Fluorescencementioning

confidence: 99%

Diversity and function of fluorescent molecules in marine organisms

Poding,

Jägers,

Huhn

et al. 2023

Preprint

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Fluorescence in marine organisms has mainly been studied in corals but is found in many different phyla such as Annelida, Crustacea, Mollusca, and Chordata. While many fluorescent proteins and molecules have been identified, very little information is available about the biological function of fluorescence. In this review, we focus on describing the occurrence of fluorescence in marine animals and the behavioural and physiological functions of fluorescent molecules based on experimental approaches. These biological functions of fluorescence range from prey and symbiont attraction, photoprotection, photoenhancement, stress mitigation, mimicry, aposematism to inter- and intraspecific communication. We provide a comprehensive list of marine taxa that utilize fluorescence, including demonstrated effects on behavioural or physiological responses. On one hand, this review describes the numerous known functions of fluorescence in anthozoans and their underlying molecular mechanisms in detail. On the other hand, it highlights which marine taxa should be further studied regarding their functions of fluorescence. We suggest that an increase in research effort in this field could contribute to understanding the capacity of marine organisms in mitigating negative effects of climate change.

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Transient‐state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria

Cited by 4 publications

References 37 publications

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

Perspectives on Bioluminescence Mechanisms

Diversity and function of fluorescent molecules in marine organisms

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