Translating bioinformatics in oncology: Guilty by profiling meta-analysis and identification of KIF18B and CDCA3 as novel driver genes in liver carcinogenesis

Itzel, Timo; Scholz, Peter; Maass, T; Krupp, Markus; Marquardt, JU; Strand, Susanne; Becker, Dorothea; Staib, Frank; Binder, Hans; Xw, Wang; Thorgeirsson, SS; Galle, PR; Teufel, Ashley I.

doi:10.1055/s-0032-1332071

Cited by 3 publications

(3 citation statements)

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“…These factors all promote tumorigenesis. As was reported, KIF18B promoted tumor development in a variety of cancers, such as cervical cancer, 20 liver cancer, 27 lung cancer, 28 and clear cell renal cell carcinoma (ccRCC), 29 whereas it was not been reported in colon adenocarcinoma.…”

Section: Discussionmentioning

confidence: 75%

<p>Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma</p>

Zhao¹,

Feng²,

Zhang³

et al. 2020

CMAR

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Background The role of kinesin superfamily proteins (KIFs) has been reported in a variety of tumors and KIFs contributed to the proliferation of cancer cells. But few studies were focus on colon adenocarcinoma. Methods Through bioinformatics analysis and immunohistochemistry (IHC) assays, the expression of KIF18B in colon adenocarcinoma tissues was determined. Stable KIF18B-depleted cell lines were constructed using lentivirus-mediated shRNA of KIF18B. Cell colony formation assay and CCK8 assay were performed to assess cell proliferation degree, and the expression level of KI67 and PCNA was used to indicate cell proliferation in vitro and verified using xenograft tumors in vivo. Results KIF18B is highly expressed in colon adenocarcinoma tissues and has a negative correlation with the prognosis and tumor grade of colon adenocarcinoma. Interfering with KIF18B inhibits cell proliferation in vitro and in vivo. Conclusion KIF18B can be used as a prognostic marker for colon adenocarcinoma and may be a therapeutic target for colon adenocarcinoma treatment.

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Section: Discussionmentioning

confidence: 75%

<p>Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma</p>

Zhao¹,

Feng²,

Zhang³

et al. 2020

CMAR

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“…Previously, CDCA3 was known to be involved in several types of cancer, such as prostate cancer, liver cancer, and oral squamous cell carcinoma [ 15 – 18 ]. In another bioinformatics study of 2158 full cancer transcriptomes from 163 diverse entities, CDCA3 was proven to be a novel gene involved in liver carcinogenesis [ 19 ]. In the current analysis, CDCA3 expression levels were high in invasive ductal breast carcinoma and were highly correlated with a low survival probability for breast cancer patients, leading to a poor prognosis.…”

Section: Discussionmentioning

confidence: 99%

Distinct expression of CDCA3, CDCA5, and CDCA8 leads to shorter relapse free survival in breast cancer patient

Phan

Wang

et al. 2018

Oncotarget

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Breast cancer is a dangerous disease that results in high mortality rates for cancer patients. Many methods have been developed for the treatment and prevention of this disease. Determining the expression patterns of certain target genes in specific subtypes of breast cancer is important for developing new therapies for breast cancer. In the present study, we performed a holistic approach to screening the mRNA expression of six members of the cell division cycle-associated gene family (CDCA) with a focus on breast cancer using the Oncomine and The Cancer Cell Line Encyclopedia (CCLE) databases. Furthermore, Gene Expression-Based Outcome for Breast Cancer Online (GOBO) was also used to deeply mine the expression of each CDCA gene in clinical breast cancer tissue and breast cancer cell lines. Finally, the mRNA expression of the CDCA genes as related to breast cancer patient survival were analyzed using a Kaplan-Meier plot. CDCA3, CDCA5, and CDCA8 mRNA expression levels were significantly higher than the control sample in both clinical tumor sample and cancer cell lines. These highly expressed genes in the tumors of breast cancer patients dramatically reduced patient survival. The interaction network of CDCA3, CDCA5, and CDCA8 with their co-expressed genes also revealed that CDCA3 expression was highly correlated with cell cycle related genes such as CCNB2, CDC20, CDKN3, and CCNB1. CDCA5 expression was correlated with BUB1 and TRIP13, while CDCA8 expression was correlated with BUB1 and CCNB1. Altogether, these findings suggested CDCA3, CDCA5, and CDCA8 could have a high potency as targeted breast cancer therapies.

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“…KIF18B encodes a protein product that regulates microtubule dynamics (i.e., microtubule length) and thus, plays an important role in cell division through its involvement in mitotic spindle assembly 18 19 20 . KIF18B has been shown to be overexpressed in hepatocellular carcinoma, and some evidence suggests that the expression of this gene may also be deregulated in several other types of tumor tissues 20 21 . Both of these genes warrant additional examination in future etiologic studies.…”

Section: Discussionmentioning

confidence: 99%

Targeted Sequencing in Chromosome 17q Linkage Region Identifies Familial Glioma Candidates in the Gliogene Consortium

Jalali

Amirian

Bainbridge

et al. 2015

Sci Rep

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Glioma is a rare, but highly fatal, cancer that accounts for the majority of malignant primary brain tumors. Inherited predisposition to glioma has been consistently observed within non-syndromic families. Our previous studies, which involved non-parametric and parametric linkage analyses, both yielded significant linkage peaks on chromosome 17q. Here, we use data from next generation and Sanger sequencing to identify familial glioma candidate genes and variants on chromosome 17q for further investigation. We applied a filtering schema to narrow the original list of 4830 annotated variants down to 21 very rare (<0.1% frequency), non-synonymous variants. Our findings implicate the MYO19 and KIF18B genes and rare variants in SPAG9 and RUNDC1 as candidates worthy of further investigation. Burden testing and functional studies are planned.

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Translating bioinformatics in oncology: Guilty by profiling meta-analysis and identification of KIF18B and CDCA3 as novel driver genes in liver carcinogenesis

Cited by 3 publications

References 0 publications

<p>Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma</p>

<p>Kinesin Superfamily Member 18B (KIF18B) Promotes Cell Proliferation in Colon Adenocarcinoma</p>

Distinct expression of CDCA3, CDCA5, and CDCA8 leads to shorter relapse free survival in breast cancer patient

Targeted Sequencing in Chromosome 17q Linkage Region Identifies Familial Glioma Candidates in the Gliogene Consortium

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