Translocation of an unusual cAMP receptor to the nucleus during development of Dictyostelium discoideum.

Kay, Carolyn A.; Noce, Toshiaki; Tsang, Adrian

doi:10.1073/pnas.84.8.2322

Cited by 17 publications

(11 citation statements)

References 46 publications

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“…We do not yet know whether p34 and p31 possess the cAMP binding activity displayed by CABPl . We have previously shown that the developmental regulation and intracellular distribution of these molecules are quite different (Kay et al, 1987). These differences suggest that these molecules do not perform exactly the same functions in the cell.…”

Section: Discussionmentioning

confidence: 91%

“…In contrast, p34 and p31 cannot be detected in growing cells by immunoblotting. They appear soon after starvation and then remain at relatively constant levels throughout development (Kay et al, 1987). We do not know the reason for the discrepancy between the expression of the mRNAs and that of the polypeptides.…”

Section: Discussionmentioning

confidence: 99%

“…A cDNA library was constructed according to standard procedures (Maniatis et al, 1982) from a mixture of polyA+ RNAs prepared from NC4 cells that had developed for 12 and 20 h as described by Kay et al (1987). The library was screened under conditions of low stringency with a nick-translated cDNA clone encoding a portion of CABPI as described by Maniatis et al (1982).…”

Section: Methodsmentioning

confidence: 99%

“…These molecules, which have apparent molecular masses of 34 kDa (p34) and 31 kDa (p31), are not detected in vegetatively growing cells, but appear early in development (Kay et al, 1987). They are localized primarily in the nucleus (Kay et al, 1987). In this paper, we describe the molecular cloning and characterization of cDNAs encoding p34 and p31.…”

Section: Introductionmentioning

confidence: 99%

“…This protein consists of two polypeptide subunits, CABPl A and CABPl B. Western blot analysis using anti-CABP 1 monoclonal antibodies indicates that there are several CABPl -related polypeptides present in developing cells (Kay et al, 1987;Tsang et al 1988).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of cDNA clones encoding polypeptides related to a Dictyostelium discoideum cyclic AMP binding protein

Bain

Grant²,

Tsang³

1991

Journal of General Microbiology

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By screening a cDNA library with a cDNA encoding the Dicfyostefium discoideum CAMP-binding protein CABP1, under conditions of reduced stringency, we have isolated clones which code for two closely related molecules. Hybrid selection experiments indicated that these cDNAs encoded polypeptides with molecular masses of 34 (p34) and 31 (p31) kDa, both of which were recognized by anti-CABP1 monoclonal antibodies. Sequence analysis revealed that the clones were identical except for the presence of a 102 nucleotide segment inserted in-frame in the p34 cDNAs, just downstream of the translation initiation codon. DNA blot analysis suggested that p34 and p31 were encoded by the same gene. This hypothesis was strongly supported by the observation that both polypeptides were generated when a single cDNA was expressed under the control of the actin 15 promoter in D. discoideum cells. RNA blot analysis indicated that the cDNAs were complementary to three developmentally regulated transcripts of sizes 1.15 kb, 1-25 kb and 1.4 kb. Comparison of the derived amino acid sequences of p34 and p31 with those of the two subunits of CABPl indicated that these polypeptides were very closely related, and that the corresponding genes probably arose by duplication followed by sequence divergence. Finally, the carboxy termini of these four polypeptides demonstrated 50% similarity to two polypeptides encoded by a bacterial plasmid which confers resistance to tellurium anions.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation and characterization of cDNA clones encoding polypeptides related to a Dictyostelium discoideum cyclic AMP binding protein

Bain

Grant²,

Tsang³

1991

Journal of General Microbiology

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Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

et al. 1988

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Several lines of evidence indicate that cAMP modulates developmental gene activity via cell-surface receptors. We describe here a novel cAMP receptor, CABP1, whose properties are consistent with the idea that this protein is involved in gene regulation. Firstly, immunological techniques using anti-CABP1 antibodies as probes showed that this cAMP receptor can be detected on the surface of developing cells. Secondly, there is a steady migration of CABP1 to the nucleus during development. Thirdly, some genetic variants exhibiting an altered pattern of development are found to possess modified CABP1. We also showed that CABP1 co-purifies with at least seven other polypeptides which share common epitopes with CABP1. Interestingly, four of the CABP1-related polypeptides can be detected on the cell surface as well as in the nucleus.

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Control of cAMP‐induced gene expression by divergent signal transduction pathways

et al. 1991

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A compilation of literature data and recent experiments led to the following conclusions regarding cyclic adenosine 3':5' monophosphate (cAMP) regulation of gene expression. Several classes of cAMP-induced gene expression can be discriminated by sensitivity to stimulation kinetics. The aggregation-related genes respond only to nanomolar cAMP pulses. The prestalk-related genes respond both to nanomolar pulses and persistent micromolar stimulation. The prespore specific genes respond only to persistent micromolar stimulation. The induction of the aggregation- and prestalk-related genes by nanomolar cAMP pulses may share a common transduction pathway, which does not involve cAMP, while involvement of the inositol 1,4,5-trisphosphate (IP3)/Ca2+ pathway is unlikely. Induction of the expression of prespore and prestalk-related genes by micromolar cAMP stimuli utilizes divergent signal processing mechanisms. cAMP-induced prespore gene expression does not involve cAMP and probably also not cyclic guanosine 3'.5' monophosphate (cGMP) as intracellular intermediate. Involvement of cAMP-induced phospholipase C (PLC) activation in this pathway is suggested by the observation that IP3 and 1,2-diacylglycerol (DAG) can induce prespore gene expression, albeit in a somewhat indirect manner and by the observation that Li+ and Ca2+ antagonists inhibit prespore gene expression. Cyclic AMP induction of prestalk-related gene expression is inhibited by IP3 and DAG and promoted by Li+, and is relatively insensitive to Ca2+ antagonists, which indicates that PLC activation does not mediate prestalk-related gene expression. Neither prespore nor prestalk-related gene expression utilizes the sustained cAMP-induced pHi increase as intracellular intermediate.

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Translocation of an unusual cAMP receptor to the nucleus during development of Dictyostelium discoideum.

Cited by 17 publications

References 46 publications

Isolation and characterization of cDNA clones encoding polypeptides related to a Dictyostelium discoideum cyclic AMP binding protein

Isolation and characterization of cDNA clones encoding polypeptides related to a Dictyostelium discoideum cyclic AMP binding protein

Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

Control of cAMP‐induced gene expression by divergent signal transduction pathways

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