Translocation of DNA of Bacterial Origin in Lycopersicum esculentum by Ultracentrifugation in Caesium Chloride Gradient

Stroun, Maurice; Anker, Philippe; Charles, P; Ledoux, L

doi:10.1038/215975b0

Cited by 30 publications

(16 citation statements)

References 2 publications

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“…Two radioactive peaks were identified that corresponded with the bacterial and the plant DNAs. More than 30% of the DNA sedimented at the bacterial site thus confirming that even after 48 h in the plant the bacterial DNA had remained intact 18 . The labeling present in the plant DNA was considered to be due to the breakdown and re‐utilization of the bacterial DNA by the host cells.…”

Section: Introductionmentioning

confidence: 77%

Circulating DNA

2006

Annals of the New York Academy of Sciences

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Section: Introductionmentioning

confidence: 77%

Circulating DNA

2006

Annals of the New York Academy of Sciences

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“…This is based primarily on two facts; i) tumor cells shed DNA into the circulation of cancer patients [38], [39] and ii) circulating DNA has the ability to enter individual cells [12], [17]–[18] and it can modify the biology of the recipient cells/organisms regardless of whether it is integrated or not [19]–[27]. In line with these data and our results, we hypothesize that in cancer patients the tumor DNA shed into the circulation is inserted into “normal-appearing” initiated stem cells, which are transformed by the circulating DNA, leading to “second primary tumors” ( Suppl.…”

Section: Discussionmentioning

confidence: 99%

“…These findings led to the concept that DNA could act as a messenger [10]–[16]. This view has been supported by the ease with which administered bacterial and eukaryote DNA can circulate freely throughout animal and plant bodies and in its ability to enter individual cells naturally, where it can locate in the host cell nuclei [12], [17]–[18]. The uptake of circulating DNA by eukaryotes shows that the biology of the recipient cells/organisms could be modified regardless of whether it is integrated or not [19]–[27].…”

Section: Introductionmentioning

confidence: 99%

Cancer Progression Mediated by Horizontal Gene Transfer in an In Vivo Model

et al. 2012

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It is known that cancer progresses by vertical gene transfer, but this paradigm ignores that DNA circulates in higher organisms and that it is biologically active upon its uptake by recipient cells. Here we confirm previous observations on the ability of cell-free DNA to induce in vitro cell transformation and tumorigenesis by treating NIH3T3 recipient murine cells with serum of colon cancer patients and supernatant of SW480 human cancer cells. Cell transformation and tumorigenesis of recipient cells did not occur if serum and supernatants were depleted of DNA. It is also demonstrated that horizontal cancer progression mediated by circulating DNA occurs via its uptake by recipient cells in an in vivo model where immunocompetent rats subjected to colon carcinogenesis with 1,2-dimethylhydrazine had increased rate of colonic tumors when injected in the dorsum with human SW480 colon carcinoma cells as a source of circulating oncogenic DNA, which could be offset by treating these animals with DNAse I and proteases. Though the contribution of biologically active molecules other than DNA for this phenomenon to occur cannot be ruled out, our results support the fact that cancer cells emit into the circulation biologically active DNA to foster tumor progression. Further exploration of the horizontal tumor progression phenomenon mediated by circulating DNA is clearly needed to determine whether its manipulation could have a role in cancer therapy.

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“…Lucien Ledoux published reports of Arabidopsis thalia plant seeds incorporating naked bacterial DNA, without the need for any specific vector or machinery (Stroun et al ., ). The newly transferred DNA corrected mutational defects (Ledoux et al .…”

Section: Microbiological Examplesmentioning

confidence: 97%

Beyond the fringe: when science moves from innovative to nonsense

Silver

2013

FEMS Microbiol Lett

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Microbiology has experienced examples of highly productive researchers who have gone beyond just interpreting their experimental results with hypotheses and published nonsense that was readily recognized as such by readers. Although the most discussed cases of this pathology come from physics, studies of single-celled microorganisms, virology, and immunology have provided many examples. Five cases are described here along with some generalizations. These are the Lamarckian inheritance of acquired characteristics reported by distinguished and experienced researchers, vectorless DNA transfer and incorporation of bacterial DNA into chromosomes of plants years before vector construction of genetically modified plants was invented, water with memory of immunoglobulin IgE, a new electromagnetic radiation method for identifying bacterial and viral pathogens by the discoverer of human immunodeficiency virus, and the claim of isolation of a new bacterial isolate with arsenic replacing phosphorus in DNA. These examples represent very dissimilar areas, and the only common factor is hubris on the part of experienced researchers. Secondarily, failure of peer review sometimes happens, and journal editors do not step in, sometimes even when alerted before publication. These failures of the publishing process teach us that unnecessary mistakes occur and should warn us all to watch our own enthusiasms.

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Translocation of DNA of Bacterial Origin in Lycopersicum esculentum by Ultracentrifugation in Caesium Chloride Gradient

Cited by 30 publications

References 2 publications

Circulating DNA

Circulating DNA

Cancer Progression Mediated by Horizontal Gene Transfer in an In Vivo Model

Beyond the fringe: when science moves from innovative to nonsense

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