Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics

Abstract

ObjectiveThis work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice. (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies. Methods Four Results A higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet. ConclusionThe addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug.

Dietary Sugars Analysis: Quantification of Fructooligossacharides during Fermentation by HPLC-RI Method

“…For instance, FOS reduce the colonization of Salmonella enteritidis , but concomitantly increase the translocation of this invasive pathogen ( 9 , 10 ). On other hand, Silva et al ( 11 ) observed that FOS reduced Klebsiella sp. translocation.…”