Translocation of the Retina for Management of Subfoveal Choroidal Neovascularization II: A Preliminary Report in Humans

Juan, Eugene de; Loewenstein, Anat; Bressler, Neil M.; Alexander, Judith

doi:10.1016/s0002-9394(98)00018-x

Cited by 194 publications

(57 citation statements)

References 16 publications

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“…While no cure has been discovered for AMD, several clinical studies have shown that RPE cell replacement may be a promising therapeutic option [3][4][5][6][7][8][9][10][11][12][13] . With the advent of stem cell technology, it is now possible to generate large numbers of RPE cells in vitro from embryonic and induced pluripotent stem cells (hES and hiPS) that strongly resemble their somatic counterparts functionally and anatomically [14][15][16][17][18][19][20][21][22][23][24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Westenskow

Kurihara

Bravo

et al. 2015

JoVE

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The conversion of light into electrical impulses occurs in the outer retina and is accomplished largely by rod and cone photoreceptors and retinal pigment epithelium (RPE) cells. RPE provide critical support for photoreceptors and death or dysfunction of RPE cells is characteristic of agerelated macular degeneration (AMD), the leading cause of permanent vision loss in people age 55 and older. While no cure for AMD has been identified, implantation of healthy RPE in diseased eyes may prove to be an effective treatment, and large numbers of RPE cells can be readily generated from pluripotent stem cells. Several interesting questions regarding the safety and efficacy of RPE cell delivery can still be examined in animal models, and well-accepted protocols used to inject RPE have been developed. The technique described here has been used by multiple groups in various studies and involves first creating a hole in the eye with a sharp needle. Then a syringe with a blunt needle loaded with cells is inserted through the hole and passed through the vitreous until it gently touches the RPE. Using this injection method, which is relatively simple and requires minimal equipment, we achieve consistent and efficient integration of stem cell-derived RPE cells in between the host RPE that prevents significant amount of photoreceptor degeneration in animal models. While not part of the actual protocol, we also describe how to determine the extent of the trauma induced by the injection, and how to verify that the cells were injected into the subretinal space using in vivo imaging modalities. Finally, the use of this protocol is not limited to RPE cells; it may be used to inject any compound or cell into the subretinal space.

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Section: Introductionmentioning

confidence: 99%

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Westenskow

Kurihara

Bravo

et al. 2015

JoVE

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“…Macular translocation is a surgical procedure in which CNV is typically removed and the macula is displaced so that the fovea overlies normal RPE (Machemer and Steinhorst 1993;Ninomiya et al 1996;de Juan et al 1998;Lai et al 2002;Mruthyunjaya et al 2004). Limited macular translocation involves partially detaching the retina via injection of subretinal fluid and shifting the inferior retina downward with the help of a gas bubble to create a retinal fold.…”

Section: Macular Surgerymentioning

confidence: 99%

Clinical Characteristics and Current Treatment of Age-Related Macular Degeneration

Yonekawa

Kim

2014

Cold Spring Harbor Perspectives in Medicine

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Age-related macular degeneration (AMD) is a multifactorial degeneration of photoreceptors and retinal pigment epithelium. The societal impact is significant, with more than 2 million individuals in the United States alone affected by advanced stages of AMD. Recent progress in our understanding of this complex disease and parallel developments in therapeutics and imaging have translated into new management paradigms in recent years. However, there are many unanswered questions, and diagnostic and prognostic precision and treatment outcomes can still be improved. In this article, we discuss the clinical features of AMD, provide correlations with modern imaging and histopathology, and present an overview of treatment strategies.

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“…Um grande ensaio clínico, multicêntrico e randomizado em 11 centros nos EUA está atualmente sendo realizado (Submacular Surgery Trials Pilot Study, com suporte do National Eye Institute dos National Institutes of Health) e irá revelar o papel real da cirurgia submacular no tratamento da membrana neovascular coroidal pela degeneração macular relacionada à idade. Outras modalidades de tratamento cirúrgico estão sendo investigadas, tais como translocação de mácula, transplante do epitélio pigmentado da retina e epitélio iriano autólogo na mácula (26)(27) . Radioterapia tem sido proposta para impedir a proliferação de células endoteliais de capilares subrretinianos recém-formados e para induzir a obliteração de neovasos na membrana neovascular coroidal.…”

Section: N T R O D U ç ã Ounclassified

Degeneração macular relacionada à idade: modalidades terapêuticas

Farah¹,

Oshima²,

Costa³

et al. 2001

Arq. Bras. Oftalmol.

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Translocation of the Retina for Management of Subfoveal Choroidal Neovascularization II: A Preliminary Report in Humans

Cited by 194 publications

References 16 publications

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Clinical Characteristics and Current Treatment of Age-Related Macular Degeneration

Degeneração macular relacionada à idade: modalidades terapêuticas

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