Transmission and total internal reflection integrated digital holographic microscopy

Abstract

We develop a transmission and total internal reflection (TIR) integrated digital holographic microscopy (DHM) by introducing a home-made Dove prism with a polished short side. With the help of angular and polarization multiplexing techniques, the 2D refractive index distribution of a specimen adhered on the prism surface is determined using TIR-DHM. Meanwhile, the thickness profile is unambiguously calculated from the phase information using transmission DHM. This integrated microscopy is nondestructive and dynamic and can be used to simultaneously measure the index distribution and thickness profile of transparent or semi-transparent liquid or solid samples.

Sb2Te3 topological insulator: surface plasmon resonance and application in refractive index monitoring

“…[57] To achieve the real-time monitoring of the refractive index variation, we set the incident angle θe as 71. to the reflection phase shift differences. [67] From Figure 5b, we can see that the reflection phase difference exhibits a nearly linear fall when the mixture droplet volatilizes from the 0 to 501 s, and then gradually rises. In the monitoring process, the phase sensitivity is lowest at the time of 501 s, which can be compensated by the highest intensity sensitivity.…”

“…[58] Fortunately, DH can simultaneously provide the intensity and phase information of the specimens with the advantages of dynamic and non-intrusive measurement. [59,67] The SPR-based DH enables to measure the near-field refractive index distribution of the specimen from the hologram recorded in the far field. [59] In DH, the intensity and phase information of the tested specimen are obtained with the angular spectrum reconstruction method (Supporting Information).…”

“…This angle is much smaller than the internal SPR angle of ~70 o in the gold-based Kretschmann configurations, contributing to the reduction of distortion for holographic images. [59,67] The SPR angle sensitivity can be defined…”

Quantitative and Dynamic Phase Imaging of Biological Cells by the Use of the Digital Holographic Microscopy Based on a Beam Displacer Unit

“…Assuming that we separately record two digital holograms in the cases without and with cells in some states, and corresponding reconstructed object waves are U 1 (ξ, η) and U 2 (ξ, η), respectively. Using double-exposure holographic interferometry [22], [23], the phase change ϕ between U 1 (ξ, η) and U 2 (ξ, η) can be calculated as…”

An advanced phase retrieval algorithm in N-step phase-shifting interferometry with unknown phase shifts

“…Phase-shifting interferometry (PSI)1, a non-intervention, high accuracy, full field, rapid speed and quantitative phase retrieval technique, has been widely used in wavefront reconstruction, optical element testing, refractive index measurement2 and quantitative phase imaging (QPI) microscopy3456, which is a tool of phase-sample evaluation in micro-scale and frequently applied in biomedicine in, e.g., red blood cell3 and cancer diagnosis4. To date, many phase-shifting algorithms (PSAs) have been developed78910111213141516171819, such as in temporal PSAs, the fixed-coefficients PSAs71011 and tunable PSAs71213 reveal rapid speed and high accuracy, but their performance is usually affected by the phase shifts deviation (PSD) induced by the detuning of phase shifter.…”