Transmission Electron Microscope Morphogram of Progressive Motile Sperm Cell Fraction Separated from Teratozoospermic Human Semen

Abstract

Teratozoospermic semen specimens were separated, with the aid of a specially designed separation chamber, into an upper progressive motile sperm fraction and a lower fraction containing a high percentage of sperm cells of poor or no motility, and immature germinal cells. Analysis of the TEM morphograms of progressive motile cell fractions and original teratozoospermic semen revealed that 15 various malformations were improved significantly in the motile cell population due to the cell separation technique.

Laboratory Efforts to Improve Motility of Human Sperm

“…By the employment of higher centrifugation speeds Eltes and colleagues 20 have designed a separation chamber for the rise technique, thus isolating about 7% of the total sperm with an average motility rate of 95%.…”