Transmission of pangola stunt virus by Sogatella kolophon

Abstract

A fijivirus causing minor enations, stunting, leaf notching, seed head deformity and excess tillering of Digitaria spp. was transmitted from naturally infected Digitaria ciliaris to D. ciliaris, D. decumbens and Urochloa panicoides by the planthopper Sogatella kolophon; 40 -70% of insects transmitted after an incubation period of 15 -21 days, and continued to transmit for up to 30 more days until death. Symptoms developed in test plants 30 -50 days after inoculation. Sogatella longifurcifera failed to transmit the virus under similar conditions. Virus particles were present in roots, stems and leaves of infected plants, and particles were found in regular arrays and random aggregates in fat body cells of transmitting insects. Viroplasm and tubular structures were associated with these particles. Extracts from infective insects contained 10-segment dsRNA when analysed by polyacrylamide gel electrophoresis. Virus survives over winter in planthoppers and D. ciliaris seedlings in frost-free areas of coastal Queensland, but infected plants have debilitated root systems and compete poorly with healthy plants.

Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes

“…Sooatella kolophon is the planthopper vector in Australia [5], but S.furcifera is the vector in South America [9]. It may be that the genome is differentially selected by different vector species.…”

“…) in which the virus apparently replicates [5]. In experimental host range studies, it has been found to infect a range of Digitaria species and Urochloa panicoides [16].…”

Insights Into Insect Vector Transmission and Epidemiology of Plant-Infecting Fijiviruses

“…The planthopper Javesella pellucida Fabricius is the natural vector of OSDV, but the leafhopper Calligypona pellucida Fabr also transmits OSDV (Milne and Lovisolo, 1977). Sogatella kolophon Kirkaldy and Sogatella furcifera were shown to vector PaSV (Greber et al, 1988;Teakle et al, 1988). SRBSDV has been identified as being transmitted by S. furcifera, but L. striatellus can also acquire it but not transmit to plants (Jia et al, 2012;Pu et al, 2012;Zhou et al, 2013).…”

“…Once inside the planthoppers, the virus multiplies during a 1-4 weeks latency period when it is also disseminating into other tissues, including the salivary glands (Greber et al, 1988). When the latency period is complete, that is, the viral titre has reached a certain level, the insects can frequently transmit virus in their lifetime (Conti, 1984;Hughes et al, 2008;Argüello Caro et al, 2013;Hajano et al, 2015).…”

Tagosodes orizicolus: nuevo vector potencial del Mal de Río Cuarto virus

“…Este tiempo fue muy superior a los 8 días de latencia mínima reportados para D. kuscheli (Arneodo et al, 2002a). Este amplio período de latencia ha sido observado también en otro Fijivirus del grupo II, el Pangola stunt virus (PaSV), por Sogatella kolophon Kirkaldy (Greber et al, 1988). Se observó mayor mortalidad (76%) de T. orizicolus durante los primeros 17 días de latencia, lo que explica la diferencia en el número de individuos entre ambas especies vectoras, al momento de iniciar las transmisiones.…”