Transmission of plasmid-borne and chromosomal blaCTX-M-64 among Escherichia coli and Salmonella isolates from food-producing animals via ISEcp1-mediated transposition

Zhao, Qiu-Yun; Chen, Pin-Xian; Yang, Ling; Cai, Run-Mao; Zhu, Jia-Hang; Fang, Liang‐Xing; Webber, Mark A.; Jiang, Hongxia

doi:10.1093/jac/dkaa044

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…The contigs carrying bla CTX-M-14 in the four chicken isolates showed high similarity to each other and to the backbone of the IncHI2 plasmid, while beef isolate 19M harbored a chromosomal bla CTX-M-14 (Figure 1C). This highlights the potential role of ISEcp1 in mobilization of bla CTX-M-14 regardless of whether the gene was located on plasmids or the chromosome (Zhao et al, 2020). Both variants of fosA, fosA3 in beef isolate 21M and fosA4 in chicken isolate 82CH, were located downstream to IS26 belonging to the IS6 family (Figures 1D, E), which might be involved in their mobilization.…”

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 93%

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Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Ramadan

Soliman

Hiott

et al. 2021

Front. Cell. Infect. Microbiol.

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In this study, multidrug-resistant (MDR) Escherichia coli isolates from retail food and humans assigned into similar Multilocus Sequence Types (MLST) were analyzed using whole genome sequencing (WGS). In silico analysis of assembled sequences revealed the existence of multiple resistance genes among the examined E. coli isolates. Of the six CTX-M-producing isolates from retail food, blaCTX-M-14 was the prevalent variant identified (83.3%, 5/6). Two plasmid-mediated fosfomycin resistance genes, fosA3, and fosA4, were detected from retail food isolates (one each from chicken and beef), where fosA4 was identified in the chicken isolate 82CH that also carried the colistin resistance gene mcr-1. The blaCTX-M-14 and fosA genes in retail food isolates were located adjacent to insertion sequences ISEcp1 and IS26, respectively. Sequence analysis of the reconstructed mcr-1 plasmid (p82CH) showed 96–97% identity to mcr-1-carrying IncI2 plasmids previously identified in human and food E. coli isolates from Egypt. Hierarchical clustering of core genome MLST (HierCC) revealed clustering of chicken isolate 82CH, co-harboring mcr-1 and fosA4 genes, with a chicken E. coli isolate from China at the HC200 level (≤200 core genome allelic differences). As E. coli co-harboring mcr-1 and fosA4 genes has only been recently reported, this study shows rapid spread of this genotype that shares similar genetic structures with regional and international E. coli lineages originating from both humans and food animals. Adopting WGS-based surveillance system is warranted to facilitate monitoring the international spread of MDR pathogens.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 93%

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Ramadan

Soliman

Hiott

et al. 2021

Front. Cell. Infect. Microbiol.

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“…IS Ecp1 appeared to be able to use IRL in combination with a sequence beyond its IRR end to move an adjacent region, yielding the transfer of the entire transposon unit [ 59 , 60 ]. IS Ecp1 -mediated transposition of bla CTX-M-64 , bla CTX-M-2 , bla CTX-M-3 , and bla TEM-1b have been demonstrated [ 61 , 62 , 63 ]. Moreover, IS Ecp1 could capture DNA regions with different sizes and simultaneously transfer adjacent regions [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

Emergence of a Hybrid IncI1-Iα Plasmid-Encoded blaCTX-M-101 Conferring Resistance to Cephalosporins in Salmonella enterica Serovar Enteritidis

Qin

Zhang

2023

Microorganisms

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The increasing resistance to cephalosporins in Salmonella poses a serious threat to public health. In our previous study, the blaCTX-M-101 gene, a new blaCTX-M variant, was first reported in Salmonella enterica serovar Enteritidis (S. Enteritidis). Here, we further analyzed the genome characterization, transferability, and resistance mechanism of one S. Enteritidis isolate (SJTUF14523) carrying blaCTX-M-101 from an outpatient in 2016 in Xinjiang, China. This strain was a multidrug resistance (MDR) isolate and exhibited resistance to ceftazidime (MIC = 64 μg/mL), cefotaxime (MIC = 256 μg/mL), and cefepime (MIC = 16 μg/mL). Phylogenetic analysis revealed that SJTUF14523 had a close relationship to another S. Enteritidis isolate from the United States. In the presence of plasmid p14523A, there were 8- and 2133-fold increases in the MICs of cephalosporins in Escherichia coli C600 in the conjugation. Gene cloning results indicated that blaCTX-M-101 was the decisive mechanism leading to ceftazidime and cefotaxime resistance that could make the MICs break through the resistance breakpoint. Plasmid sequencing revealed that the blaCTX-M-101 gene was located on an IncI1-Iα transferable plasmid (p14523A) that was 85,862 bp in length. Sequence comparison showed that p14523A was a novel hybrid plasmid that might have resulted from the interaction between a homologous region. Furthermore, we found a composite transposon unit composed of ISEcp1, blaCTX-M-101, and orf477 in p14523A. ISEcp1-mediated transposition was likely to play a key role in the horizontal transfer of blaCTX-M-101 among plasmids in S. Enteritidis. Collectively, these findings underline further challenges in the prevention and control of antibiotic resistance posed by new CTX-M-101-like variants in Salmonella.

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“…The pap2 gene was always present downstream of mcr-1 except for QH20-4D2-1 contig_81 (JAJDKI010000081), which contained bla CTX-M-64 , an independent resistance region of the ESBL resistance gene. This region was located in ISEcp1-bla CTX-M-64 -Δorf477, a typical transposon unit, of which the ISEcp1 insertion sequence can mediate the transposition of many types of the bla CTX-M gene (Zhao et al, 2020). Except in the case of QH20-4B1-2 contig_139 (JAJDKH010000139), the resistance gene mcr-1 was integrated at a location downstream to the nikB gene, which is identical to that of the majority of mcr-1-positive IncI2-type plasmids reported previously.…”

Section: Id Antibiotic Resistance Pattern Number Percentage (%) Amp-a...mentioning

confidence: 99%

Antimicrobial resistance surveillance of Escherichia coli from chickens in the Qinghai Plateau of China

Tang

Wang²,

Zheng³

et al. 2022

Front. Microbiol.

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Antimicrobial resistance (AMR) may lead to worldwide epidemics through human activities and natural transmission, posing a global public safety threat. Colistin resistance mediated by the mcr-1 gene is the most prevalent among animal-derived Escherichia coli, and mcr-1-carrying E. coli have been frequently detected in central-eastern China. However, animal-derived E. coli with AMR and the prevalence of mcr-1 in the Qinghai Plateau have been rarely investigated. Herein, 375 stool samples were collected from 13 poultry farms in Qinghai Province and 346 E. coli strains were isolated, of which eight carried mcr-1. The AMR rates of the E. coli strains to ampicillin, amoxicillin/clavulanic acid, and tetracycline were all above 90%, and the resistance rates to ciprofloxacin, cefotaxime, ceftiofur, and florfenicol were above 70%. Multidrug-resistant strains accounted for 95.66% of the total isolates. Twelve E. coli strains showed colistin resistance, from which a total of 46 AMR genes and 36 virulence factors were identified through whole-genome sequencing. The mcr-1 gene resided on the IncHI2, IncI2-type and IncY-type plasmids, and mcr-1 was located in the nikA-nikB-mcr-1-pap2 gene cassette (three strains) or the pap2-mcr-1-ISApl1 structure (one strain). Completed IncI2-type plasmid pMCR4D31–3 sequence (62,259 bp) revealed that it may cause the horizontal transmission of mcr-1 and may increase the risk of its spread through the food chain. Taken together, the AMR of chicken-derived E. coli in the plateau is of concern, suggesting that it is very necessary for us to strengthen the surveillance in various regions under the background of one health.

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Transmission of plasmid-borne and chromosomal blaCTX-M-64 among Escherichia coli and Salmonella isolates from food-producing animals via ISEcp1-mediated transposition

Cited by 9 publications

References 18 publications

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Emergence of Multidrug-Resistant Escherichia coli Producing CTX-M, MCR-1, and FosA in Retail Food From Egypt

Emergence of a Hybrid IncI1-Iα Plasmid-Encoded blaCTX-M-101 Conferring Resistance to Cephalosporins in Salmonella enterica Serovar Enteritidis

Antimicrobial resistance surveillance of Escherichia coli from chickens in the Qinghai Plateau of China

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