Transmission of Potato Purple Top Phytoplasma to Potato Tubers and Daughter Plants

Abstract

, and 2008 Russet, Shepody, and Umatilla Russet potatoes were planted at a research farm near Moxee, WA. Plants were allowed to become infested with native populations of the beet leafhopper which are known vectors of the Columbia Basin potato purple top phytoplasma, a member of phytoplasma group 16SrVI, in this region. Symptomatic plants were tested by PCR for the presence of the phytoplasma and positive plants were identified with flags. Tubers were harvested from positive plants and tested for the presence of phytoplasma. A subset of phytoplasmapositive tubers was planted in a greenhouse and the resulting daughter plants were tested for phytoplasma. The frequency of tuber transmission ranged from 4% to 96% among the eight cultivars over the 3 year period. Transmission of the phytoplasma from infected tubers to daughter plants ranged from 0% to 50% depending upon cultivar and year. In two of the 3 years, Russet Burbank showed the lowest rate of tuber transmission among the eight cultivars. Across the eight cultivars an average of 22%, 15%, and 16% of infected tubers gave rise to infected daughter plants in 2006, 2007, and 2008, respectively. Resumen Se plantaron en un campo experimental cerca de Moxee, WA, en 2006, 2007, y 2008, papas de las variedades Alturas, RussetBurbank, FL1867, FL 1879, RussetNorkotah, RangerRusset, Shepody y UmatillaRusset. Se permitió que las plantas se infestaran con poblaciones nativas de chicharritas del betabel, que se sabe son vectores del fitoplasma de la punta morada de la papa, miembro del grupo de fitoplasmas 16SrVI, en la región de la rivera del Columbia. A las plantas sintomáticas se les hizo la prueba de PCR para la presencia del fitoplasma, y a las positivas se les identificó con banderas. Se cosecharon los tubérculos de las plantas positivas y se probaron para la presencia del fitoplasma. Se plantó un subgrupo de tubérculos positivos en invernadero y las plantas resultantes se probaron para fitoplasmas. La frecuencia de transmisión por tubérculo tuvo una amplitud entre el 4 y el 96% entre las ocho variedades a lo largo del periodo de los tres años. La transmisión del fitoplasma de tubérculos infectados a plantas hijas varió de 0 a 50% dependiendo de la variedad y del año. En dos de los tres años, RussetBurbank mostró el nivel más bajo de transmisión por tubérculo entre las ocho variedades. Considerando todas las variedades, se obtuvo un promedio de 22%, 15% y 16% de tubérculos infectados que dieron origen a plantas hijas enfermas en 2006 a 2008, respectivamente.

Potato (Solanum tuberosum) as a New Host for Pentastiridius leporinus (Hemiptera: Cixiidae) and Candidatus Arsenophonus Phytopathogenicus

“…The prevalence of both pathogens in different regions of Germany is still unknown and must be assessed to facilitate appropriate containment measures. Seed potatoes could potentially spread both diseases [ 33 ]. Potential control strategies could include population suppression in P. leporinus and the modification of crop rotations to avoid consecutive crops of potato and sugar beet.…”

Seed degeneration in potato: the need for an integrated seed health strategy to mitigate the problem in developing countries

“…Seedborne insect pests, such as potato tuber moth, can also be considered important causes of degeneration in tropical countries, as they may readily become seedborne and have multiple generations in the field and in storage (Sileshi & Teriessa, ; Golizadeh et al ., ). Similarly, newly discovered viruses (Li et al ., ), as well as emerging epidemics of Potato yellow vein virus in many South American countries (Salazar et al ., ), potato purple top phytoplasma in the Pacific Northwest of the United States (Crosslin et al ., ) and zebra chip disease (‘ Candidatus Liberibacter solanacearum’) in many countries (Henne et al ., ) may develop into significant seed degeneration threats.…”

Molecular detection and characterization of a stolbur phytoplasma associated with Narcissus tazetta phyllody in Iran

“…P. solani” has been previously reported in different parts of Iran infecting several plants such as almond (Zirak, Bahar, & Ahoonmanesh, ), plum (Zirak, Bahar, & Ahoonmanesh, ), peach (Zirak, Bahar, & Ahoonmanesh, ), potato (Hosseini, Bahar, Madani, & Zirak, ), cannabis (Sichani, Bahar, & Zirak, ) and grapevine (Mirchenari, Massah, & Zirak, ). Phytoplasmas usually can colonize different parts of a plant and move from one part to another (Crosslin, Hamlin, Buchman, & Munyaneza, ; Khadhair, Duplessis McAlister, Ampong‐Nyarko, & Bains, ; Munyaneza & Crosslin, ; Weintraub & Jones, ). Thus, the phytoplasma can move from narcissus plant to its bulb and bulbous and infect future plants.…”