Transparent, conductive, and SERS-active Au nanofiber films assembled on an amphiphilic peptide template

Zarzhitsky, Shlomo; Morag, Ahiud; Zeiri, Leila; Levi‐Kalisman, Yael; Rapaport, Hanna; Jelinek, Raz

doi:10.1039/c3nr03348a

Cited by 29 publications

(24 citation statements)

References 41 publications

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“…Beyond 1-D structures, noble metal catalysts with 2-D structures can also be obtained by employing a peptide template whose nanofibers can undergo further associations to form nanofilms. 55,56 One such example is provided by Jelinek and his co-workers, who synthesized transparent and conductive Au nanofiber films on a peptide monolayer. 56 After peptide P FK -5 ((Phe-Lys) 5 -Pro) self-assembled into a nanofilm, a TEM grid was used to load the peptide film and then floated over an aqueous solution of Au(SCN) 4 1– .…”

Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

“…55,56 One such example is provided by Jelinek and his co-workers, who synthesized transparent and conductive Au nanofiber films on a peptide monolayer. 56 After peptide P FK -5 ((Phe-Lys) 5 -Pro) self-assembled into a nanofilm, a TEM grid was used to load the peptide film and then floated over an aqueous solution of Au(SCN) 4 1– . The negatively charged Au complexes bind to the peptide film and then reduce into metallic Au.…”

Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

“…After that, the nanofiber networks of Au are fabricated. 56 According to such study, peptide-templated synthesis is an effective approach to fabricate noble metal catalysts with 2-D structures. However, due to the requirements of peptide self-assembly, this approach usually needs a longer operation time, which severely limits its development.…”

Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

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Peptide-templated noble metal catalysts: syntheses and applications

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Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

Section: Synthesis Of Peptide-templated Noble Metal Catalystsmentioning

confidence: 99%

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Peptide-templated noble metal catalysts: syntheses and applications

et al. 2017

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“…We have recently demonstrated that Au(SCN) 4 − selfassembles into conductive Au 0 nanostructures through singlestep crystallization/reduction process which do not require addition of reducing agents. [23][24][25] Here we show that a surfactant, octyl-melamine (OM), adopts elongated and aligned micro-wire morphology when isothermally compressed at the air/water interface. Furthermore, the OM assemblies constitute a template for gold deposition through electrostatic attraction between the primary amine residues of the surfactant and soluble Au(SCN) 4 − , accompanied by spontaneous crystallization and reduction of gold by the thiocyanate moieties, without addition of reducing agents.…”

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confidence: 98%

Spontaneous Assembly of Extremely Long, Horizontally‐Aligned, Conductive Gold Micro‐Wires in a Langmuir Monolayer Template

Jiang

Jelinek

2014

Adv Materials Inter

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The propensity of Au ions to self-assemble and undergo rapid reduction into colloids and NPs, however, poses distinct barriers for utilizing conventional gold chemistry for creating structurally-defi ned, organized wire structures and thin fi lms. Patterned Au structures have been produced through chemical derivation of gold colloids surfaces [ 14 ] and/or the substrate employed for surface deposition, [15][16][17] or through the aid of physical templates. [18][19][20][21][22] These approaches exhibit shortcomings, both conceptually as well as technically. Specifi cally, Au NP synthesis and surface derivatization are often multi-step procedures and introduce certain variability in term of product uniformity. In addition, in many cases chemical treatment of NPbased fi lms is further required after surface deposition of the NPs, presenting additional experimental parameters to modify and optimize. Furthermore, on a fundamental basis, the rapid reduction of ionic gold into metal NPs severely restricts the possibility to control the structural features and spatial, long-range organization of the metal assemblies.We present a simple strategy for fabricating extremely long (up to several centimeters), horizontally-aligned, conductive gold micro-wires, produced through deposition of a surfactant monolayer upon gold thiocyanate [Au(SCN) 4 − ] aqueous solution. We have recently demonstrated that Au(SCN) 4 − selfassembles into conductive Au 0 nanostructures through singlestep crystallization/reduction process which do not require addition of reducing agents. [23][24][25] Here we show that a surfactant, octyl-melamine (OM), adopts elongated and aligned micro-wire morphology when isothermally compressed at the air/water interface. Furthermore, the OM assemblies constitute a template for gold deposition through electrostatic attraction between the primary amine residues of the surfactant and soluble Au(SCN) 4 − , accompanied by spontaneous crystallization and reduction of gold by the thiocyanate moieties, without addition of reducing agents. In particular, previous studies have demonstrated that immobilization of the gold complex onto amine-displaying surfaces leads to crystalline organization that is largely promoted through aurophilic interactions among Au I species. Reduction leading to metallic gold formation was found to occur concurrently with the crystallization process, overall resulting in surface-anchored Au 0 nanostructure "Bottom-up" technologies are based upon the premise that organized systems -from the nano-scale up to the macro-scale -can be assembled spontaneously from basic building blocks in solution. We demonstrate a simple strategy for the generation of extremely long (up to several centimeters), horizontally-aligned gold micro-wires, produced through a surfactant monolayer template deposited from gold thiocyanate [Au(SCN) 4 − ] aqueous solution. Specifi cally, we show that the surfactant, octyl-maleimide (OM), spontaneously forms oriented micro-wires at the air/water interface, which constitute a templat...

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“…88 This peptide was also shown to template calcium phosphate mineralisation, making it potentially applicable in bone tissue regeneration. Vinod et al 109 recently reported the peptide P FK -5, which incorporated the cationic lysine as the charged hydrophilic residue. This peptide assembled at air-water interfaces and was able to template gold nanofibre film formation.…”

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Bioproduction and self-assembly of designer peptides

Fletcher¹

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Self-assembly is a powerful method for producing controlled morphologies at the nanometre scale. Peptides are biomolecules capable of self-assembly and have the potential for use in a variety of applications such as emulsion and foam stabilisation, wound healing and drug delivery. The investigation of peptide sequence-structure relationships is a rapidly advancing field of study, which in many cases now allows the targeted design of self-assembling peptides.While self-assembling peptides show potential in several fields, their large-scale adoption is limited by the high production expenses associated with conventional solid-phase synthesis. A potentially cheaper and more renewable approach to peptide production is bacterial expression. However, the bioproduction of peptides is a non-trivial process, and generally involves the expression of peptides as part of a fusion protein in which the target peptide is only a small portion of the expressed product. An alternate approach involves peptide concatemers, in which the target peptide makes up the majority of the expressed construct.The work reported in this thesis focused on the design, characterisation and bioproduction of self-assembling α-helical peptides. It was particularly focussed on the development of amphiphillic α-helical peptides with applications as surfactants and hydrogelators. The aim of this work was to further the field of de novo peptide design, while also investigating an approach for peptide bioproduction. This work aimed to combine the requirements for peptide bioproduction with end-use functionality.Chapter 2 details successful bioproduction of an anionic helical surfactant peptide EDP-11, as part of a charge-paired heteroconcatemer with the cationic expression partner RDP-4. The method utilised designed assembly of the constituent α-helical peptides to generate a stably folded coiled-coil concatemer. The polypeptide sequence was further optimized for molecular charge, hydropathy and predicted protease resistance. This process allowed expression of a soluble concatemer that accumulated to high levels (22% of total protein) in E. coli. The expressed concatemer possessed extreme stability to heat and proteases, allowing isolation by simple heat and pH precipitation, yielding concatemer at 130 mg per gram of dry cell weight and >99% purity. Key parameters used in designing the heteroconcatemer were then compared to those of all open reading frames of several reference ii proteomes in an attempt to gain insight into the mechanistic basis for the high stability of the designed miniprotein.Following bioproduction using this concatemer approach, further processing was required to produce monomeric peptides for use in surfactant applications. The design of acid-cleavable aspartate-proline sites within the concatemer sequence allowed for simple heat-and acid-mediated cleavage to give constituent peptides.Chapter 3 details characterization of cleavage of the expressed concatemer by coupled liquid chromatography-mass spectrometry and includes mod...

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Transparent, conductive, and SERS-active Au nanofiber films assembled on an amphiphilic peptide template

Cited by 29 publications

References 41 publications

Peptide-templated noble metal catalysts: syntheses and applications

Peptide-templated noble metal catalysts: syntheses and applications

Spontaneous Assembly of Extremely Long, Horizontally‐Aligned, Conductive Gold Micro‐Wires in a Langmuir Monolayer Template

Bioproduction and self-assembly of designer peptides

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