Afforestation of agriculturally used land has many benefits which are described in different publications. As stated in these publications, afforestation of agricultural land can influence soil water content because of rainfall interception and concentration, transpiration, and properties of forest floor layer and soil; also, hydraulic lift may occur. In this study, we attempted to find changes in volumetric soil water content values (at depths of 20 cm, 40 cm, and 60 cm) on agriculturally used land afforested with a mixture of forest tree species (Quercus robur L., Quercus rubra L., and Acer platanoides L.) or Pinus sylvestris L. in the year 2011 (and on agriculturally used land = control). This study was performed in the period from the end of March 2021 to the beginning of April 2022. In this study, we proved our hypothesis on the reduction of volumetric soil water content after afforestation; it is probably because of higher rainfall interception and evapotranspiration as described in different studies. Concerning the afforestation, the average volumetric water content was higher on the plot afforested with broadleaves compared with Scots pine. It was especially in the period after defoliation when the proportion of throughfall (and stemflow) increased on the plot with broadleaves. Our results indicate a higher water-storage capacity of forests compared with agriculturally used land. During the leafless period, this capacity was found to be higher after afforestation with Scots pine.