Transplantation of autologous cells and porous gelatin microcarriers to promote wound healing

Larsson, Alexander; Briheim, Kristina; Hanna, Victor Fouad; Gustafsson, Karin; Starkenberg, Annika; Vintertun, Hans N.; Kratz, Gunnar; Junker, Johan P.E.

doi:10.1016/j.burns.2020.08.003

Cited by 7 publications

(8 citation statements)

References 31 publications

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“…Additionally, although keratinocytes were detected after skin grafting in rats, the implantation of a layer of these cells to an acellular dermal matrix did not affect the dermal thickness in the wound healing process [34]. Moreover, the thickness of the neo-epidermis after 4 weeks in pigs treated with scaffolds (porous gelatin microcarriers) without or with cells (keratinocytes and fibroblasts) was similar, although in inferior time points a difference has been detected [35]. Also, a scaffold produced with 3-hydroxybutyrate/4hydroxybutyrate showed that both cell-free and fibroblast-seeded membranes facilitated wound healing, evaluated by histologic examination and measurements of the wound surface area [36].…”

Section: Bilayer Scaffold Improves Granulation Tissue Formationmentioning

confidence: 91%

Bilayer scaffold from PLGA/fibrin electrospun membrane and fibrin hydrogel layer supports wound healing in vivo

et al. 2023

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Hybrid scaffolds from natural and synthetic polymers have been widely used due to the complementary nature of their physical and biological properties. The aim of the present study, therefore, has been to analyze in vivo a bilayer scaffold of poly(lactide-co-glycolide) (PLGA)/fibrin electrospun membrane and fibrin hydrogel layer on a rat skin model. Fibroblasts were cultivated in the fibrin hydrogel layer and keratinocytes on the electrospun membrane to generate a skin substitute. The scaffolds without and with cells were tested in a full-thickness wound model in Wistar Kyoto rats. The histological results demonstrated that the scaffolds induced granulation tissue growth, collagen deposition and epithelial tissue remodeling. The wound-healing markers showed no difference in scaffolds when compared with the positive control. Activities of antioxidant enzymes were decreased concerning the positive and negative control. The findings suggest that the scaffolds contributed to the granulation tissue formation and the early collagen deposition, maintaining an anti-inflammatory microenvironment.

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Section: Bilayer Scaffold Improves Granulation Tissue Formationmentioning

confidence: 91%

Bilayer scaffold from PLGA/fibrin electrospun membrane and fibrin hydrogel layer supports wound healing in vivo

et al. 2023

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“…In Vitro Swelling, Degradation, and Release Kinetics. To investigate the swelling behavior of microcarriers in vitro, dry microcarriers (W 0 ) were soaked in 5 mL of deionized water with pH = 7.4, and the deionized water was absorbed at 5,10,15,20,25,30,35,40,60,120, and 180 min, respectively. After weighing (W s ), the swelling rate swelling (%) = (W s − W 0 )/W 0 × 100% was calculated.…”

Section: Preparation Of Microcarriersmentioning

confidence: 99%

“…First introduced by van Wezel in 1967, microcarriers have been commonly used to scale-up cell production . Although the use of microcarriers in skin flap repair has been limited, they have been used for the regeneration of numerous tissues such as skin, bone, cartilage, and nerves due to their favorable cell and drug loading properties. − Direct delivery of autologous or allogeneic cells to skin wounds by microcarriers can be therapeutic, however, issues including autoimmune rejection and ethical concerns limit their clinical applicability. , The flap repair process requires the continuous action of relevant cells and cytokines. Therefore, mobilizing endogenous cell migration and recruitment is an alternative approach.…”

Section: Introductionmentioning

confidence: 99%

“…31−34 Direct delivery of autologous or allogeneic cells to skin wounds by microcarriers can be therapeutic, however, issues including autoimmune rejection and ethical concerns limit their clinical applicability. 35,36 The flap repair process requires the continuous action of relevant cells and cytokines. Therefore, mobilizing endogenous cell migration and recruitment is an alternative approach.…”

Section: Introductionmentioning

confidence: 99%

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SDF-1 Functionalized Hydrogel Microcarriers for Skin Flap Repair

Liu

Wang

et al. 2022

ACS Biomater. Sci. Eng.

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Critically sized skin flaps used to treat skin defects often suffer from necrosis due to insufficient blood supply. Hence there is an urgent need to improve the survival rate of skin flaps by promoting local angiogenesis. The delivery of growth factor loaded microcarriers have shown promise in enhancing defect repair, however, their rapid clearance from the defect site limits their regenerative potential. Thus, it is critical to develop microcarriers which can promote the sustained release of bioactive factors to effectively stimulate tissue repair. This study aimed to develop a stromal cell-derived factor 1 (SDF-1) loaded microcarrier coated with Matrigel (MC@SDF-1@Mat) to promote skin flap repair. SEM imaging showed that the surface of the microcarrier was coated by a porous Matrigel film. The drug release experiment showed that the Matrigel-coated microcarriers enhanced the sustained release of the model drug methylene blue when compared to uncoated group. MC@SDF-1@Mat significantly promoted the proliferation, migration, and angiogenesis of HUVECs via CCK-8, wound healing assay, and tube formation assay, respectively. Moreover, the murine random skin flap model was further established and treated. It was found that the flap necrosis area in the MC@SDF-1@Mat treated group was significantly reduced. H&E and Masson staining showed the histological structure and collagen organization exhibited a normal phenotype in the MC@SDF-1@Mat treated group. Additionally, CD31 immunohistochemical analysis showed that the MC@SDF-1@Mat treated group exhibited the greatest degree of neovascularization. In conclusion, our SDF-1 functionalized gelatin-based hydrogel microcarrier has potential clinical applications in promoting skin flap repair and drug delivery.

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“…46 Similarities between pig and human skin, wound models, wound sizes as well as methods of analysis employed are important variables that influence healing and myofibroblast physiology. 24,[47][48][49][50][51][52][53][54][55][56] Pig and human skin appear similar histologically, with human epidermis thickness ranging from $50 to 120 μm, compared to the pig at 30-140 μm. 57 Further parallels include dermal collagen structure, well-developed dermal papillary bodies as well as subdermal adipose tissue.…”

Section: Porcine Skin-similarities and Differences With Humansmentioning

confidence: 99%

The pig as a model system for investigating the recruitment and contribution of myofibroblasts in skin healing

Hamilton

Walker

Tinney

et al. 2021

Wound Repair Regeneration

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In the skin-healing field, porcine models are regarded as a useful analogue for human skin due to their numerous anatomical and physiological similarities. Despite the widespread use of porcine models in skin healing studies, the initial origin, recruitment and transition of fibroblasts to matrix-secreting contractile myofibroblasts are not well defined for this model. In this review, we discuss the merit of the pig as an animal for studying myofibroblast origin, as well as the challenges associated with assessing their contributions to skin healing. Although a variety of wound types (incisional, partial thickness, full thickness, burns) have been investigated in pigs in attempts to mimic diverse injuries in humans, direct comparison of human healing profiles with regards to myofibroblasts shows evident differences. Following injury in porcine models, which often employ juvenile animals, myofibroblasts are described in the developing granulation tissue at 4 days, peaking at Days 7-14, and persisting at 60 days post-wounding, although variations are evident depending on the specific pig breed. In human wounds, the presence of myofibroblasts is variable and does not correlate with the age of the wound or clinical contraction. Our comparison of porcine myofibroblast-mediated healing processes with those in humans suggests that further validation of the pig model is essential. Moreover, we identify several limitations evident in experimental design that need to be better controlled, and standardisation of methodologies would be beneficial for the comparison and interpretation of results. In particular, we discuss anatomical location of the wounds, their size and depth, as well as the healing microenvironment (wet vs. moist vs. dry

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Transplantation of autologous cells and porous gelatin microcarriers to promote wound healing

Cited by 7 publications

References 31 publications

Bilayer scaffold from PLGA/fibrin electrospun membrane and fibrin hydrogel layer supports wound healing in vivo

Bilayer scaffold from PLGA/fibrin electrospun membrane and fibrin hydrogel layer supports wound healing in vivo

SDF-1 Functionalized Hydrogel Microcarriers for Skin Flap Repair

The pig as a model system for investigating the recruitment and contribution of myofibroblasts in skin healing

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