Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression

Abstract

BackgroundBone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism.MethodsAcute lung injury model was induced by middle cerebral artery occlusion (MCAO). BMSCs (with concentration of 1 × 106/ml) were transplanted into host through tail vein 1 day after MCAO. Then, the survival, proliferation and migration of BMSCs in lung were observed at 4 days after transplantation, and histology observation and lung function were assessed for 7 days. Meanwhile, in situ hybridization (ISH), qRT-PCR and western blotting were employed to detect the expression of TNF-α in lung.ResultsNeurobehavioral deficits and acute lung injury could be seen in brain ischemia rats. Implanted BMSCs could survive in the lung, and relieve pulmonary edema, improve lung function, as well as down regulate TNF-α expression.ConclusionsThe grafted BMSCs can survive and migrate widespread in lung and ameliorate lung injury induced by focal cerebral ischemia in the MCAO rat models. The underlying molecular mechanism, at least partially, is related to the suppression of TNF-α.

Microarray expression profiles of genes in lung tissues of rats subjected to focal cerebral ischemia-induced lung injury following bone marrow-derived mesenchymal stem cell transplantation

“…In previous studies, it was found that the growth status of BMSCs in passage 3 was better than that in passage 1 or 2 (25,26). Therefore, BMSCs at passage 3 were used in this study.…”

TGF-β1 is Involved in Vitamin D-Induced Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Regulating the ERK/JNK Pathway

“…Primary BMSCs were acquired as described previously [20]. In brief, the femur and tibias of SD rats were carefully separated and washed with phosphate-buffered saline (PBS).…”

“…Bone marrow-derived mesenchymal stem cells (BMSCs) possess pluripotency during several passages, and are commonly acceptable for differentiation into many cell types, such as chondrocytes and osteoblasts [9-11]. Transplantation of BMSCs has been widely studied as promising treatments for ischemia-induced pulmonary injury [12], pelvic floor dysfunction [13] and olfactory disorder [14]. In terms of orthopaedic researches, BMSCs are proved to facilitate cartilage regeneration because of the rapid cell proliferation, multipotency and easy isolation [15].…”

Bone marrow-derived mesenchymal stem cells overexpressing miR-21 efficiently repair myocardial damage in rats

“…The BMSCs differentiate into cardiomyocytes and replace the apoptotic myocardium thereby improving the function of damaged hearts [ 9 ]. They are critical for immunomodulatory function, inflammatory response and epithelial function [ 10 ]. However, the poor long-term survival of transplanted MSCs presents a major therapeutic problem that needs to be overcome [ 11 ].…”