Transplantation of bone marrow‐derived neurospheres into guinea pig cochlea

Abstract

The present findings indicate that BMSC-derived spheres can be a source for replacement of spiral ganglion neurons, although further manipulations are required for functional recovery.

Potential of Pluripotent Stem Cells for the Replacement of Inner Ears

“…The enhanced survival of BMSCs was confirmed in deafened cochleae [26]. Autologous BMSC-derived neurospheres transplanted into the cochlear modiolus of the deafened guinea pigs settled predominantly in the internal acoustic meatus [28]. Combined with those findings, BMSCs can be a source for replacement of SGNs.…”

“…We review previous reports and discuss obstacles to overcome for successful functional recovery. Several kinds of pluripotent stem cells have been delivered into the cochlea for the regeneration of SGNs, including NSCs [10,13,46], ESCs [4,5,11,12,29,34,36,38], bone marrow stem cells (BMSCs) [26,28,40], and iPSCs [27]. Tamura et al, evaluated the ability of NSCs to achieve neural differentiation in the modiolus of the cochlea and demonstrated that some grafted NSCs expressed β-III tubulin, a neuronal marker, although the majority of them differentiated into glial cells [46].…”

“…BMSCs, which can be readily obtained from an individual's own bone marrow, are also good candidates as transplants, because recent studies have shown that BMSCs can produce not only osteoblasts, chondrocytes, adipocytes, or myoblasts, but also neurons [15,16]. The survival of autologous BMSCs grafted in the cochlea was proven [26,28,40]. The enhanced survival of BMSCs was confirmed in deafened cochleae [26].…”

Análise histológica e funcional de xenotransplante de células-tronco da orelha interna de camundongos em cócleas de cobaias com perda auditiva induzida

“…Em contrapartida, a cóclea é considerada um sítio imunologicamente privilegiado, pois apresenta uma imunoproteção natural, a barreira hematolabiríntica (Hildebrand et al, 2005). Por este motivo, não houve qualquer reação inflamatória tecidual compatível com reação enxertohospedeiro nos trabalhos em que foram usados xenotransplantes (Cho et al, 2011;Pandit et al, 2011;Ogita et al, 2010;Hu et al, 2009;Lang et al, 2008;Matsuoka et al, 2007;Coleman et al, 2006;Hildebrand et al, 2005). Carlsbad, CA, USA).…”

“…Atualmente o transplante de CT para o tratamento da surdez é considerado um tratamento promissor. Nos últimos anos, as CT mais utilizadas experimentalmente para este fim são as de medula óssea (Ogita et al, 2010), neurais (Parker et al, 2007) e embrionárias (Corrales et al, 2006).…”