Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice: Immunohistochemical and electron microscopic studies on the myelination

Extensive Oligodendrocyte Remyelination following Injection of Cultured Central Nervous System Cells into Demyelinating Lesions in Adult Central Nervous System

“…Our finding of extensive oligodendrocyte remyelination of axons throughout lesions, which remain demyelinated in control animals, demonstrates the potential of transplanted oligodendro cytes and oligodendrocyte precursor cells to effect remyelination in adult animals. These observations extend previous experiments which have demonstrated that transplanted oligodendrocytes can myelinate axons in neonatal [Gumpel et al, 1983[Gumpel et al, , 1987Gansmüller et al, 1986;Duncan et al, 1987] and 1-month-old mutant animais [Kohsaka et al, 1985;Friedman et al, 1986], Further more, our results indicate that the irradiated, ethidium bromide lesion provides a neutral, in vivo environment in which to examine the interactions of cultured glial cell populations with axons in adult animals.…”

“…The establishment of normal oligodendrocytes within the CNS of 1-month-old shiverer mice has also been ef fected using suspension grafts of isolated neonatal oligodendrocytes [Kohsaka et al, 1985] and embryonic CNS cells [Friedman et al, 1986]. Transplantation of suspensions of CNS tissue has also been shown to result in the formation of myelin internodes in the my elin-deficient mutant rat [Duncan et al, 1987].…”

Transplantation of labeled fetal spinal cord fragments into juvenile myelin‐deficient rat spinal cord

“…Clusters of myelinated fibers were found subsequently in the recipient spinal cord, and, by fluorescence microscopy, clusters of FB-labeled cells were found a t corresponding sites. The results indicate that the surgical approach used is suitable for transplantation of tissue fragments into a defined region of juvenile rat spinal cord, that FB can be used to locate the transplanted cells subsequently, and that FB does not interfere with maturation of the donor glia or with myelin formation.Studies of myelinogenesis after transplantation have been done using two myelin-deficient mutant rodents, the shiverer mouse (Gumpel et al, 1983;Lachapelle et al, 1984;Gansmuller et al, 1986;Kohsaka et al, 1986;Baulac et al, 1987) and the myelin-deficient (md) rat deficient (Duncan e t al., 1988;Rosenbluth et al, 1989). Myelin in these animals is morphologically abnormal and is characterized by the lack of myelin basic protein in shiverer and proteolipid protein in md rat.…”

Functional Capacities of Transplanted Cell-Sorted Adult Oligodendrocytes

“…However this m utant is less than ideal as it is necessary to create focal areas of myelin loss prior to the injection of cultured cells [9]. More recently, the shiverer (shi) mouse, which lacks myelin basic protein, has been extensively used by Gumpel [7,10] and others [11,12] to document the myelinating and migrating ca-pacity of transplanted glial cells. A deficiency of proteolipid protein in the myelin deficient (md) rat and an almost complete lack of CNS myelin, has enabled this mutant to be an excellent recipient of grafted cells [8].…”