Transplantation of labeled fetal spinal cord fragments into juvenile myelin‐deficient rat spinal cord

Hasegawa, Mitsuhiro; Rosenbluth, Jack

doi:10.1002/ar.1092290116

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…Numerous studies have been described in the literature reporting oligodendrocyte grafts into mice and rat brain and spinal cord (Hasegawa and Rosenbluth, 1991;Rosenfeld, 1993;see Franklin and Barnet, 1997 [this issue]). Those studies, however, did not fully characterize the changes produced in md hosts by the presence of the graft.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of CG4 oligodendrocyte progenitor cells in the myelin-deficient rat brain results in myelination of axons and enhanced oligodendroglial markers

et al. 1997

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Transplantation of oligodendrocyte (Ol) progenitor cells into the central nervous system is a promisingapproach for the treatment of myelin disorders. This approach requires providing adequate numbers of healthy cells with myelinating potential. We recently showed the successful transplantation of Ol progenitors into the myelin-deficient (md) rat brain. In the present work, CG4 cells, a cell line with properties of Ol progenitors, were labeled with fast blue and grafted into P3-P5 pups born to carrier mothers. Examination of host brains 2 weeks posttransplant indicated that CG4 cells display a much more extensive migration capacity than their wild-type counterparts. These cells synthesized myelin components. In addition, ultrastructural analysis showed myelin formation along axons of md hosts in various brain regions, including corpus callosum, cerebellum, and brainstem. Furthermore, in situ hybridization studies performed on sagittal sections revealed extensive expression of transferrin-mRNA within the md host parenchyma. The high survival and functional features displayed by CG4 cells after transplantation, together with their striking wide distribution within the host parenchyma, as assessed by the presence of myelinated fibers in mutant hosts, emphasizes the importance of using highly motile and proliferative Ol progenitor cells. Strategies to improve the condition and life span of md rat pups are currently under investigation.

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Section: Discussionmentioning

confidence: 99%

Transplantation of CG4 oligodendrocyte progenitor cells in the myelin-deficient rat brain results in myelination of axons and enhanced oligodendroglial markers

et al. 1997

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“…The discrepancy about cell mi gration between Rosenbluth's findings and ours may be due to the fact that the spinal cord matures earlier than the brain, since myelination starts from caudal to rostral areas. This more mature environment may be responsible for the lack of migration of the implanted cells described by Rosenbluth et al [29] and Hasegawa and Rosenbluth [30]. Alternatively, the differences may be due to the nature of graft used.…”

Section: Fb* Cell Migration and Expression O Fmentioning

confidence: 84%

Transplantation of Cultured Premyelinating Oligodendrocytes into Normal and Myelin-Deficient Rat Brain

Zhang¹,

Gordon²,

Aymie³

et al. 1992

Dev Neurosci

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Cultures of oligodendroglial cells at various stages of maturation, from progenitors to maturing oligodendrocytes, were prepared from neonatal rat brain primary cultures and then were prelabeled in the culture dish with the fluorescent dye, fast blue (FB). Single cell suspensions were grafted into normal or myelin-deficient rat brains. The normal as well as the myelin-deficient in vivo environment allowed cell survival, migration, and differentiation. The FB⁺ cells expressed the oligodendroglial markers, glycerol phosphate dehydrogenase, galactocerebroside, and myelin basic protein. In the normal rat transplanted cells were identifiable at all times studied up to 24 weeks. Extensive migration of FB⁺ cells was observed in whole-brain sagittal sections. Our results show that the plasticity of oligodendroglia differentiation, extensively studied in vitro, can now be investigated in the normal and myelin-deficient in vivo environment.

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Distribution and morphology of transgenic mouse oligodendroglial‐lineage cells following transplantation into normal and myelin‐deficient rat CNS

Schiff

Rosenbluth

Dou

et al. 2002

J of Comparative Neurology

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Glial cells from neonatal M␤P5 transgenic mice, which express bacterial ␤-galactosidase (lacZ) under control of the myelin basic protein (MBP) promoter (Gow et al, 1992), were transplanted into the spinal cord or cerebral hemisphere of immunosuppressed normal and myelin-deficient (md) rats in order to assess the ability of the donor cells to survive, migrate, and differentiate within normal compared with myelin-deficient central nervous system (CNS). LacZϩ cells were detected as early as 6 -7 days after transplantation into the low thoracic cord and by 10 days had spread rostrally to the brainstem and caudally to the sacral spinal cord. Initially, compact lacZϩ cells, lacking processes, were found associated with small blood vessels and with the glia limitans. Cells of this type persisted throughout the experiment. Later, lacZϩ cells with processes were seen along fiber tracts in the dorsal columns and, after intracerebral injection, subjacent to ventricular ependyma, as well as scattered in cerebral white and gray parenchyma. The extent of spread was comparable in md and normal rats, but in the md group, the success rate was higher, and more cells differentiated into process-bearing oligodendrocytes. Acceptance of xenografts in immunosuppressed recipients equaled that of allografts. The overall spread of grafted cells exceeded that of injected charcoal, indicating active migration. In contrast to earlier studies that identified oligodendrocytes based on morphology alone, this study has allowed us to identify and track oligodendrocytes based on myelin gene expression. We show some oligodendrocytes whose morphology is consistent with classical morphological descriptions, some that resemble astrocytes, and a class of compact perivascular oligodendrocyte-lineage cells that we suggest are migratory.

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Transplantation of labeled fetal spinal cord fragments into juvenile myelin‐deficient rat spinal cord

Cited by 9 publications

References 17 publications

Transplantation of CG4 oligodendrocyte progenitor cells in the myelin-deficient rat brain results in myelination of axons and enhanced oligodendroglial markers

Transplantation of CG4 oligodendrocyte progenitor cells in the myelin-deficient rat brain results in myelination of axons and enhanced oligodendroglial markers

Transplantation of Cultured Premyelinating Oligodendrocytes into Normal and Myelin-Deficient Rat Brain

Distribution and morphology of transgenic mouse oligodendroglial‐lineage cells following transplantation into normal and myelin‐deficient rat CNS

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