Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff®-11) into cartilage defects in rabbits

Grigolo, Brunella; Roseti, Livia; Fiorini, M.; Fini, Milena; Giavaresi, Gianluca; Aldini, N. Nicoli; Giardino, Roberto; Facchini, Andrea

doi:10.1016/s0142-9612(00)00429-4

Cited by 245 publications

(148 citation statements)

References 36 publications

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“…Among the innovative, so called 'cell-based' therapies, a promising approach involves engineering cartilage grafts using scaffolds seeded with cells, possibly pre-cultured in vitro before implantation. A number of studies performed in rabbit (Ball et al, 2004;Grigolo et al, 2001;Schaefer et al, 2002), sheep (Kandel et al, 2006), or goat (Niederauer et al, 2000) aimed at investigating the outcome of cartilage or osteochondral repair using engineered cartilage. Cells were seeded in the scaffolds either directly after isolation or after an expansion phase and further pre-cultured in vitro for 48 h (Niederauer et al, 2000), 7 d (Ball et al, 2004), 4 to 6 weeks (Schaefer et al, 2002), or 8 weeks (Kandel et al, 2006) before implantation in an orthotopic site.…”

Section: Introductionmentioning

confidence: 99%

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Miot

Brehm²,

Dickinson³

et al. 2012

eCM

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This study was designed to determine if the maturation stage of engineered cartilage implanted in a goat model of cartilage injury infl uences the repair outcome. Goat engineered cartilage was generated from autologous chondrocytes cultured in hyaluronic acid scaffolds using 2 d, 2 weeks or 6 weeks of pre-culture and implanted above hydroxyapatite/hyaluronic acid sponges into osteochondral defects. Control defects were left untreated or treated with cell-free scaffolds. The quality of repair tissues was assessed 8 weeks or 8 months post implantation by histological staining, modifi ed O'Driscoll scoring and biochemical analyses. Increasing pre-culture time resulted in progressive maturation of the grafts in vitro. After 8 weeks in vivo, the quality of the repair was not improved by any treatment. After 8 months, O'Driscoll histology scores indicated poor cartilage architecture for untreated (29.7 ± 1.6) and cell-free treated groups (24.3 ± 5.8). The histology score was improved when cellular grafts were implanted, with best scores observed for grafts pre-cultured for 2 weeks (16.3 ± 5.8). As compared to shorter pre-culture times, grafts cultured for 6 weeks (histology score: 22.3 ± 6.4) displayed highest type II/I collagen ratios but also inferior architecture of the surface and within the defect, as well as lower integration with native cartilage. Thus, pre-culture of engineered cartilage for 2 weeks achieved a suitable compromise between tissue maturity and structural/integrative properties of the repair tissue. The data demonstrate that the stage of development of engineered cartilage is an important parameter to be considered in designing cartilage repair strategies.

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Section: Introductionmentioning

confidence: 99%

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Miot

Brehm²,

Dickinson³

et al. 2012

eCM

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“…In particular, goat articular chondrocytes were expanded in the absence or presence of growth factors previously shown to enhance bovine and human chondrocyte growth and maintenance of the ability to redifferentiate. [12][13][14] Using the identified expansion conditions, the capacity of expanded goat chondrocytes to form 3D cartilaginous tissues of predefined size and shape was then investigated using two types of scaffolds: (1) HYAFF 1 -M nonwoven meshes, made of a hyaluronic acid derivative, known to support redifferentiation of human articular chondrocytes 15 and already used for cartilage repair in rabbits 16 and humans, 3 and (2) poly(ethylene glycol)-terephtalate/poly(butylene)-terephtalate (PEGT/PBT) foams, generally referred to as Polyactive TM and recently used for cartilage tissue engineering in a variety of models. [17][18][19] …”

Section: Introductionmentioning

confidence: 99%

Cartilage tissue engineering by expanded goat articular chondrocytes

Miot

Freitas

Wirz

et al. 2006

Journal Orthopaedic Research

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In this study we investigated whether expanded goat chondrocytes have the capacity to generate cartilaginous tissues with biochemical and biomechanical properties improving with time in culture. Goat chondrocytes were expanded in monolayer with or without combinations of FGF-2, TGF-b1, and PDGFbb, and the postexpansion chondrogenic capacity assessed in pellet cultures. Expanded chondrocytes were also cultured for up to 6 weeks in HYAFF 1 -M nonwoven meshes or Polyactive TM foams, and the resulting cartilaginous tissues were assessed histologically, biochemically, and biomechanically. Supplementation of the expansion medium with FGF-2 increased the proliferation rate of goat chondrocytes and enhanced their postexpansion chondrogenic capacity. FGF-2-expanded chondrocytes seeded in HYAFF 1 -M or Polyactive TM scaffolds formed cartilaginous tissues with wet weight, glycosaminoglycan, and collagen content, increasing from 2 days to 6 weeks culture (up to respectively 2-, 8-, and 41-fold). Equilibrium and dynamic stiffness measured in HYAFF 1 -M-based constructs also increased with time, up to, respectively, 1.3-and 16-fold. This study demonstrates the feasibility to engineer goat cartilaginous tissues at different stages of development by varying culture time, and thus opens the possibility to test the effect of maturation stage of engineered cartilage on the outcome of cartilage repair in orthotopic goat models. ß

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“…Es un soporte que in vitro ha demostrado su capacidad de mantener el fenotipo de los condrocitos y estimular la producción de matriz extracelular (Grigolo, 2002). Datos provenientes de ensayos in vivo, indican que promueven la regeneración del cartílago regenerativos (Aigner et al, 1998;Grigolo et al, 2001). Hyalo-graft-C, es otro soporte de ácido hialurónico autorizado para aplicación en humanos.…”

Section: Productounclassified

Terapias Celulares y Productos de Ingeniería de Tejidos para el Tratamiento de Lesiones Condrales de Rodilla

Cabrera

Duque²,

Fontanilla

2017

Rev. colomb. biotecnol.

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RESUMENEl cartílago articular es un tejido vulnerable a las lesiones de diferente etiología; siendo uno de los más afectados, el cartílago de la rodilla. Aunque la mayoría de los tratamientos convencionales reducen los síntomas, generalmente conducen a la formación de fibrocartílago; el cual, posee características diferentes a las del cartílago hialino de las articulaciones. Son pocas las aproximaciones terapéuticas que promueven el reemplazo del tejido dañado por cartílago hialino funcional; las más exitosas son las denominadas terapias avanzadas, que aplican células y productos de ingeniería de tejidos con el fin de estimular la regeneración del cartílago. La mayoría de ellas se basan en colocar soportes hechos con biomateriales de diferente origen, que sembrados o no con células exógenas o endógenas, reemplazan al cartílago dañado y promueven su regeneración. Este trabajo revisa algunas de las aproximaciones terapéuticas enfocadas en la regeneración del cartílago articular de rodilla; así como, los biomateriales más empleados en la elaboración de soportes para terapia celular e ingeniería de tejido cartilaginoso.Palabras clave: biomaterial, cartílago, lesiones de rodilla, ingeniería de tejidos. ABSTRACTThe articular cartilage is prone to suffer lesions of different etiology, being the articular cartilage lesions of the knee the most common. Although most conventional treatments reduce symptoms they lead to the production of fibrocartilage, which has different characteristics than the hyaline cartilage of the joint. There are few therapeutic approaches that promote the replacement of damaged tissue by functional hyaline cartilage. Among them are the so-called advanced therapies, which use cells and tissue engineering products to promote cartilage regeneration. Most of them are based on scaffolds made of different biomaterials, which seeded or not with endogenous or exogenous cells, can be used as cartilage artificial replacement to improve joint function. This paper reviews some therapeutic approaches focused on the regeneration of articular cartilage of the knee and the biomaterials used to develop scaffolds for cell therapy and tissue engineering of cartilage. ARTÍCULO DE REVISIÓNRev.

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Transplantation of chondrocytes seeded on a hyaluronan derivative (Hyaff®-11) into cartilage defects in rabbits

Cited by 245 publications

References 36 publications

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Influence of in vitro maturation of engineered cartilage on the outcome of osteochondral repair in a goat model

Cartilage tissue engineering by expanded goat articular chondrocytes

Terapias Celulares y Productos de Ingeniería de Tejidos para el Tratamiento de Lesiones Condrales de Rodilla

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